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AB42633

Recombinant Human HDAC8 protein

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Recombinant Human HDAC8 protein is a Human Full Length protein, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for Inhib.

View Alternative Names

HDACL1, CDA07, HDAC8, Histone deacetylase 8, HD8, Protein deacetylase HDAC8, Protein decrotonylase HDAC8

Key facts

Purity

>90% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

Tag free

Applications

Inhib

applications

Biologically active

No

Accession

Q9BY41

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.8004% Sodium chloride, 0.79% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Inhib": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Expressed in a Baculovirus infected Sf9 cell expression system. MW= 46.4 kDa. 10 U/ug. One U =1 pmol/min, Assay condition: 25 mM Tris/Cl, pH8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 100 uM Biomol substrate (Catalog number KI-178), and 15 ng/ul HDAC8. Incubation condition: 60 min at 30ºC.
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Sequence info

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q9BY41","tags":[]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein HDAC8 also known as Histone Deacetylase 8 functions mechanically by removing acetyl groups from histone tails influencing chromatin structure and gene expression. HDAC8 has a molecular mass of roughly 41 kDa. It expresses in various tissues but shows higher expression in smooth muscle cells and certain cancer cell lines. Researchers also note its expression in the brain and liver corresponding to sections that require its deacetylase activity for various regulatory functions.
Biological function summary

HDAC8 plays a role in regulating transcription by being involved in altering chromatin structure. It does not form part of a large multi-protein complex unlike some other histone deacetylases. Its activity affects cell cycle progression and differentiation which is central to cellular physiology. HDAC8 also participates in non-histone protein deacetylation impacting various substrates allowing it to govern diverse cellular processes.

Pathways

Scientists associate HDAC8 with cell cycle regulation and muscle contraction. HDAC8 plays a role in the molecular pathways that control how myosin light chains undergo deacetylation interacting with proteins such as smooth muscle actin. During cell cycle regulation HDAC8 affects p53 a protein critical in controlling cell cycle progression and apoptosis integrating into pathways that decide cellular outcomes during division and response to stress.

Researchers identify HDAC8 as having connections to certain cancers and Cornelia de Lange Syndrome (CdLS). Its deregulation links to tumorigenesis particularly in colorectal and neuroblastoma cancers where it may interact with oncogenes and tumor suppressors. In CdLS mutations that affect HDAC8 lead to developmental issues with the disorder being connected to the cohesin complex proteins which highlight HDAC8's importance in chromosomal cohesion and gene expression regulation.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Histone deacetylase that catalyzes the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) (PubMed : 10748112, PubMed : 10922473, PubMed : 10926844, PubMed : 14701748, PubMed : 28497810). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events (PubMed : 10748112, PubMed : 10922473, PubMed : 10926844, PubMed : 14701748). Histone deacetylases act via the formation of large multiprotein complexes (PubMed : 10748112, PubMed : 10922473, PubMed : 10926844, PubMed : 14701748). Also involved in the deacetylation of cohesin complex protein SMC3 regulating release of cohesin complexes from chromatin (PubMed : 22885700). May play a role in smooth muscle cell contractility (PubMed : 15772115). In addition to protein deacetylase activity, also has protein-lysine deacylase activity : acts as a protein decrotonylase by mediating decrotonylation ((2E)-butenoyl) of histones (PubMed : 28497810).

Sequence similarities

Belongs to the histone deacetylase family. HD type 1 subfamily.

Post-translational modifications

Phosphorylated by PKA on serine 39. Phosphorylation reduces deacetylase activity observed preferentially on histones H3 and H4.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Histone deacetylase that catalyzes the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) (PubMed : 10748112, PubMed : 10922473, PubMed : 10926844, PubMed : 14701748, PubMed : 28497810). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events (PubMed : 10748112, PubMed : 10922473, PubMed : 10926844, PubMed : 14701748). Histone deacetylases act via the formation of large multiprotein complexes (PubMed : 10748112, PubMed : 10922473, PubMed : 10926844, PubMed : 14701748). Also involved in the deacetylation of cohesin complex protein SMC3 regulating release of cohesin complexes from chromatin (PubMed : 22885700). May play a role in smooth muscle cell contractility (PubMed : 15772115). In addition to protein deacetylase activity, also has protein-lysine deacylase activity : acts as a protein decrotonylase by mediating decrotonylation ((2E)-butenoyl) of histones (PubMed : 28497810).
See full target information HDAC8
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