Recombinant human HDAC8 protein (Active) is a Human Full Length protein, in the 1 to 377 aa range, expressed in Baculovirus infected Sf9, with >80% purity and suitable for SDS-PAGE, FuncS.
Images
Key facts
- Purity
- >80% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
M E E P E E P A D S G Q S L V P V Y I Y S P E Y V S M C D S L A K I P K R A S M V H S L I E A Y A L H K Q M R I V K P K V A S M E E M A T F H T D A Y L Q H L Q K V S Q E G D D D H P D S I E Y G L G Y D C P A T E G I F D Y A A A I G G A T I T A A Q C L I D G M C K V A I N W S G G W H H A K K D E A S G F C Y L N D A V L G I L R L R R K F E R I L Y V D L D L H H G D G V E D A F S F T S K V M T V S L H K F S P G F F P G T G D V S D V G L G K G R Y Y S V N V P I Q D G I Q D E K Y Y Q I C E S V L K E V Y Q A F N P K A V V L Q L G A D T I A G D P M C S F N M T P V G I G K C L K Y I L Q W Q L A T L I L G G G G Y N L A N T A R C W T Y L T G V I L G K T L S S E I P D H E F F T A Y G P D Y V L E I T P S C R P D R N E P H R I Q Q I L N Y I K G N L K H V V
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Histone deacetylase that catalyzes the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) (PubMed:10748112, PubMed:10922473, PubMed:10926844, PubMed:14701748, PubMed:28497810). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events (PubMed:10748112, PubMed:10922473, PubMed:10926844, PubMed:14701748). Histone deacetylases act via the formation of large multiprotein complexes (PubMed:10748112, PubMed:10922473, PubMed:10926844, PubMed:14701748). Also involved in the deacetylation of cohesin complex protein SMC3 regulating release of cohesin complexes from chromatin (PubMed:22885700). May play a role in smooth muscle cell contractility (PubMed:15772115). In addition to protein deacetylase activity, also has protein-lysine deacylase activity: acts as a protein decrotonylase by mediating decrotonylation ((2E)-butenoyl) of histones (PubMed:28497810).
Alternative names
HDACL1, CDA07, HDAC8, Histone deacetylase 8, HD8, Protein deacetylase HDAC8, Protein decrotonylase HDAC8
Recommended products
Recombinant human HDAC8 protein (Active) is a Human Full Length protein, in the 1 to 377 aa range, expressed in Baculovirus infected Sf9, with >80% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >80% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- The specific activity of HDAC8 was determined to be 1249 RLU/min/ng in a histone deacetylase assay.
- Accession
- Q9BY41-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7
Preservative: 1.02% Imidazole
Constituents: 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride, 1.05% MOPS, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF
Sequence info
Amino acid sequence
- M E E P E E P A D S G Q S L V P V Y I Y S P E Y V S M C D S L A K I P K R A S M V H S L I E A Y A L H K Q M R I V K P K V A S M E E M A T F H T D A Y L Q H L Q K V S Q E G D D D H P D S I E Y G L G Y D C P A T E G I F D Y A A A I G G A T I T A A Q C L I D G M C K V A I N W S G G W H H A K K D E A S G F C Y L N D A V L G I L R L R R K F E R I L Y V D L D L H H G D G V E D A F S F T S K V M T V S L H K F S P G F F P G T G D V S D V G L G K G R Y Y S V N V P I Q D G I Q D E K Y Y Q I C E S V L K E V Y Q A F N P K A V V L Q L G A D T I A G D P M C S F N M T P V G I G K C L K Y I L Q W Q L A T L I L G G G G Y N L A N T A R C W T Y L T G V I L G K T L S S E I P D H E F F T A Y G P D Y V L E I T P S C R P D R N E P H R I Q Q I L N Y I K G N L K H V V
- Accession
- Q9BY41
- Protein length
- Full Length
- Amino acids
- 1 to 377
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Liquid
General info
- Function
Histone deacetylase that catalyzes the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) (PubMed:10748112, PubMed:10922473, PubMed:10926844, PubMed:14701748, PubMed:28497810). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events (PubMed:10748112, PubMed:10922473, PubMed:10926844, PubMed:14701748). Histone deacetylases act via the formation of large multiprotein complexes (PubMed:10748112, PubMed:10922473, PubMed:10926844, PubMed:14701748). Also involved in the deacetylation of cohesin complex protein SMC3 regulating release of cohesin complexes from chromatin (PubMed:22885700). May play a role in smooth muscle cell contractility (PubMed:15772115). In addition to protein deacetylase activity, also has protein-lysine deacylase activity: acts as a protein decrotonylase by mediating decrotonylation ((2E)-butenoyl) of histones (PubMed:28497810).
- Sequence similarities
Belongs to the histone deacetylase family. HD type 1 subfamily.
- Post-translational modifications
Phosphorylated by PKA on serine 39. Phosphorylation reduces deacetylase activity observed preferentially on histones H3 and H4.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein HDAC8 also known as Histone Deacetylase 8 functions mechanically by removing acetyl groups from histone tails influencing chromatin structure and gene expression. HDAC8 has a molecular mass of roughly 41 kDa. It expresses in various tissues but shows higher expression in smooth muscle cells and certain cancer cell lines. Researchers also note its expression in the brain and liver corresponding to sections that require its deacetylase activity for various regulatory functions.
- Biological function summary
HDAC8 plays a role in regulating transcription by being involved in altering chromatin structure. It does not form part of a large multi-protein complex unlike some other histone deacetylases. Its activity affects cell cycle progression and differentiation which is central to cellular physiology. HDAC8 also participates in non-histone protein deacetylation impacting various substrates allowing it to govern diverse cellular processes.
- Pathways
Scientists associate HDAC8 with cell cycle regulation and muscle contraction. HDAC8 plays a role in the molecular pathways that control how myosin light chains undergo deacetylation interacting with proteins such as smooth muscle actin. During cell cycle regulation HDAC8 affects p53 a protein critical in controlling cell cycle progression and apoptosis integrating into pathways that decide cellular outcomes during division and response to stress.
- Associated diseases and disorders
Researchers identify HDAC8 as having connections to certain cancers and Cornelia de Lange Syndrome (CdLS). Its deregulation links to tumorigenesis particularly in colorectal and neuroblastoma cancers where it may interact with oncogenes and tumor suppressors. In CdLS mutations that affect HDAC8 lead to developmental issues with the disorder being connected to the cohesin complex proteins which highlight HDAC8's importance in chromosomal cohesion and gene expression regulation.
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2 product images
Functional Studies - Recombinant human HDAC8 protein (Active) (ab268619)
The specific activity of HDAC8 was determined to be 1249 RLU/min/ng in a histone deacetylase assay.
SDS-PAGE - Recombinant human HDAC8 protein (Active) (ab268619)
SDS-PAGE analysis of ab268619.
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