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AB131907

Recombinant Human HEC1/HEC protein

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Recombinant Human HEC1/HEC protein is a Human Full Length protein, in the 1 to 642 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

HEC, HEC1, KNTC2, NDC80, Kinetochore protein NDC80 homolog, Highly expressed in cancer protein, Kinetochore protein Hec1, Kinetochore-associated protein 2, Retinoblastoma-associated protein HEC, HsHec1

1 Images
SDS-PAGE - Recombinant Human HEC1/HEC protein (AB131907)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human HEC1/HEC protein (AB131907)

12.5% SDS-PAGE analysis of ab131907 stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

Tag free

Applications

ELISA, SDS-PAGE, WB

applications

Biologically active

No

Accession

O14777

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as HEC1.
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Sequence info

[{"sequence":"MKRSSVSSGGAGRLSMQELRSQDVNKQGLYTPQTKEKPTFGKLSINKPTSERKVSLFGKRTSGHGSRNSQLGIFSSSEKIKDPRPLNDKAFIQQCIRQLCEFLTENGYAHNVSMKSLQAPSVKDFLKIFTFLYGFLCPSYELPDTKFEEEVPRIFKDLGYPFALSKSSMYTVGAPHTWPHIVAALVWLIDCIKIHTAMKESSPLFDDGQPWGEETEDGIMHNKLFLDYTIKCYESFMSGADSFDEMNAELQSKLKDLFNVDAFKLESLEAKNRALNEQIARLEQEREKEPNRLESLRKLKASLQGDVQKYQAYMSNLESHSAILDQKLNGLNEEIARVELECETIKQENTRLQNIIDNQKYSVADIERINHERNELQQTINKLTKDLEAEQQKLWNEELKYARGKEAIETQLAEYHKLARKLKLIPKGAENSKGYDFEIKFNPEAGANCLVKYRAQVYVPLKELLNETEEEINKALNKKMGLEDTLEQLNAMITESKRSVRTLKEEVQKLDDLYQQKIKEAEEEDEKCASELESLEKHKHLLESTVNQGLSEAMNELDAVQREYQLVVQTTTEERRKVGNNLQRLLEMVATHVGSVEKHLEEQIAKVDREYEECMSEDLSENIKEIRDKYEKKATLIKSSEE","proteinLength":"Full Length","predictedMolecularWeight":"100.3 kDa","actualMolecularWeight":null,"aminoAcidEnd":642,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"O14777","tags":[]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HEC1 also known as NDC80 or HEC is an important component of the kinetochore a protein complex importantly involved in chromosome segregation during cell division. It is often referred to as HEC-1 with a molecular mass of approximately 80 kDa. HEC1 is expressed ubiquitously in proliferating cells and it plays an important role in the accurate alignment and segregation of chromosomes during mitosis. The protein is found at centromeres where it is essential for proper attachment of microtubules.
Biological function summary

In the context of cell division HEC1 functions as part of the NDC80 complex a critical ensemble of four proteins that include NUF2 SPC24 and SPC25. This complex is vital for microtubule binding and kinetochore function. HEC1 as a subunit of NDC80 ensures kinetic stability and maintains the structural integrity of the kinetochore during the cell cycle. Its function is necessary for accurate chromosome alignment on the metaphase plate which prevents missegregation and aneuploidy.

Pathways

HEC1 is actively involved in the spindle assembly checkpoint (SAC) a safety mechanism that prevents anaphase onset until all chromosomes are properly aligned. This pathway is important in regulating cell cycle progression and preventing genomic instability. HEC1 also interacts with proteins like BUB1 and MAD2 which are integral to the SAC pathway. HEC1’s associations highlight its role in maintaining mitotic accuracy and integrity in dividing cells.

HEC1 has a significant connection to cancer. Aberrant expression or malfunction of HEC1 contributes to chromosomal instability a hallmark of cancer progression. Its interactions with oncogenes and tumor suppressor proteins like TP53 can exacerbate this instability leading to unregulated cell division and tumor growth. Additionally HEC1's role in the cell cycle and proliferation links it to disorders like aneuploidies where chromosome number errors occur further implicating its importance in maintaining chromosomal balance.
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Specifications

Form

Liquid

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General info

Function

Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity (PubMed : 12351790, PubMed : 14654001, PubMed : 14699129, PubMed : 15062103, PubMed : 15235793, PubMed : 15239953, PubMed : 15548592, PubMed : 16732327, PubMed : 30409912, PubMed : 9315664). Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore (PubMed : 15548592, PubMed : 30409912). The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules (PubMed : 23085020). Plays a role in chromosome congression and is essential for the end-on attachment of the kinetochores to spindle microtubules (PubMed : 23891108, PubMed : 25743205).

Sequence similarities

Belongs to the NDC80/HEC1 family.

Post-translational modifications

Phosphorylation begins in S phase of the cell cycle and peaks in mitosis. Phosphorylated by NEK2. Also phosphorylated by AURKA and AURKB.. Acetylated at Lys-53 and Lys-59 by KAT5 during mitosis, promoting robust kinetochore-microtubule attachment (PubMed:30409912). Deacetylated by SIRT1 (PubMed:30409912).

Subcellular localisation

Nucleus

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Product protocols

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Target data

Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity (PubMed : 12351790, PubMed : 14654001, PubMed : 14699129, PubMed : 15062103, PubMed : 15235793, PubMed : 15239953, PubMed : 15548592, PubMed : 16732327, PubMed : 30409912, PubMed : 9315664). Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore (PubMed : 15548592, PubMed : 30409912). The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules (PubMed : 23085020). Plays a role in chromosome congression and is essential for the end-on attachment of the kinetochores to spindle microtubules (PubMed : 23891108, PubMed : 25743205).
See full target information NDC80
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Complementary Products

Primary Antibodies

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Anti-HEC1/HEC antibody [EPR5342]

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Western blot - Anti-HEC1/HEC antibody [EPR5342] (AB109496)

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Primary Antibodies

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Western blot - Anti-CENPE antibody [EPR4542(2)] (AB133583)

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Primary Antibodies

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Anti-HURP antibody

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Primary Antibodies

AB193666

Anti-CENPC antibody [EPR15939]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CENPC antibody [EPR15939] (AB193666)

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