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AB161805

Recombinant Human HECTD1 protein (GST tag N-Terminus)

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Recombinant Human HECTD1 protein (GST tag N-Terminus) is a Human Fragment protein, in the 3 to 110 aa range, expressed in Wheat germ, suitable for ELISA, WB.

View Alternative Names

KIAA1131, HECTD1, E3 ubiquitin-protein ligase HECTD1, E3 ligase for inhibin receptor, HECT domain-containing protein 1, EULIR

1 Images
SDS-PAGE - Recombinant Human HECTD1 protein (GST tag N-Terminus) (AB161805)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human HECTD1 protein (GST tag N-Terminus) (AB161805)

ab161805 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

ELISA, WB

applications

Biologically active

No

Accession

Q9ULT8

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Complementary Products

Primary Antibodies

AB101992

Anti-HECTD1 antibody

Immunoprecipitation - Anti-HECTD1 antibody (AB101992)

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Proteins & Peptides

AB159886

Recombinant Human ZP2 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human ZP2 protein (GST tag N-Terminus) (AB159886)

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Proteins & Peptides

AB162402

Recombinant Human Zinc finger protein 691 (GST tag N-Terminus)

SDS-PAGE - Recombinant Human Zinc finger protein 691 (GST tag N-Terminus) (AB162402)

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Proteins & Peptides

AB161146

Recombinant Human BS69 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human BS69 protein (GST tag N-Terminus) (AB161146)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"DVDPDTLLEWLQMGQGDERDMQLIALEQLCMLLLMSDNVDRCFETCPPRTFLPALCKIFLDESAPDNVLEVTARAITYYLDVSAECTRRIVGVDGAIKALCNRLVVVE","proteinLength":"Fragment","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":110,"aminoAcidStart":3,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q9ULT8","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HECTD1 which stands for HECT Domain E3 Ubiquitin Protein Ligase 1 also known by other names such as KIAA1594 is an important enzyme in the protein degradation process. HECTD1 acts mechanically as an E3 ubiquitin ligase which means it helps attach ubiquitin molecules to specific protein substrates marking them for degradation by the proteasome. The protein typically weighs around 261 kDa and expresses broadly in human tissues including nervous system cardiovascular tissues and epithelial cells indicating its diverse functional roles.
Biological function summary

The enzyme HECTD1 contributes to numerous cellular processes notably protein homeostasis and cellular signaling. It acts as a part of a larger ubiquitination complex interacting with various adaptor proteins to achieve substrate specificity. By regulating the fate of proteins within the cell HECTD1 impacts processes like cell cycle progression apoptosis and stress responses. Studies show that it also plays a role in embryonic development pointing to its function in tissue morphogenesis and organogenesis.

Pathways

HECTD1 has significant roles within the ubiquitin-proteasome pathway and the cell cycle regulatory pathway. It interacts with proteins such as SMAD2 in the TGF-beta signaling pathway which controls cell proliferation and differentiation. This enzyme also coordinates with TRAF6 in immune signaling pathways influencing immune responses. These interactions highlight HECTD1's pivotal role in maintaining cellular function by modulating protein stability and activity.

Mutations or deregulation of HECTD1 associate with neurodevelopmental disorders and cancer. Abnormal HECTD1 activity can disrupt normal brain development linking it to certain neurodevelopmental disorders. In cancer altered expression or function of HECTD1 affects cell proliferation pathways leading to uncontrolled growth. Researchers find HECTD1 interacting with proteins like c-MYC in oncogenic pathways further establishing its relevance in the pathophysiology of human diseases.
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Specifications

Form

Liquid

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General info

Function

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates (PubMed : 33711283). Mediates 'Lys-63'-linked polyubiquitination of HSP90AA1 which leads to its intracellular localization and reduced secretion (By similarity). Negatively regulating HSP90AA1 secretion in cranial mesenchyme cells may impair their emigration and may be essential for the correct development of the cranial neural folds and neural tube closure (By similarity). Catalyzes ubiquitination and degradation of ZNF622, an assembly factor for the ribosomal 60S subunit, in hematopoietic cells, thereby promoting hematopoietic stem cell renewal (PubMed : 33711283).

Sequence similarities

Belongs to the UPL family. K-HECT subfamily.

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Product protocols

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Target data

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates (PubMed : 33711283). Mediates 'Lys-63'-linked polyubiquitination of HSP90AA1 which leads to its intracellular localization and reduced secretion (By similarity). Negatively regulating HSP90AA1 secretion in cranial mesenchyme cells may impair their emigration and may be essential for the correct development of the cranial neural folds and neural tube closure (By similarity). Catalyzes ubiquitination and degradation of ZNF622, an assembly factor for the ribosomal 60S subunit, in hematopoietic cells, thereby promoting hematopoietic stem cell renewal (PubMed : 33711283).
See full target information HECTD1
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