Recombinant Human Heme Oxygenase 1 protein is a Human Full Length protein, in the 1 to 288 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, WB.
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Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, WB
- Biologically active
- No
Amino acid sequence
M E R P Q P D S M P Q D L S E A L K E A T K E V H T Q A E N A E F M R N F Q K G Q V T R D G F K L V M A S L Y H I Y V A L E E E I E R N K E S P V F A P V Y F P E E L H R K A A L E Q D L A F W Y G P R W Q E V I P Y T P A M Q R Y V K R L H E V G R T E P E L L V A H A Y T R Y L G D L S G G Q V L K K I A Q K A L D L P S S G E G L A F F T F P N I A S A T K F K Q L Y R S R M N S L E M T P A V R Q R V I E E A K T A F L L N I Q L F E E L Q E L L T H D T K D Q S P S R A P G L R Q R A S N K V Q D S A P V E T P R G K P P L N T R S Q A P L L R W V L T L S F L V A T V A V G L Y A M
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Heme oxygenase 1. Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron (PubMed:11121422, PubMed:19556236, PubMed:7703255). Affords protection against programmed cell death and this cytoprotective effect relies on its ability to catabolize free heme and prevent it from sensitizing cells to undergo apoptosis (PubMed:20055707). Heme oxygenase 1. (Microbial infection) During SARS-COV-2 infection, promotes SARS-CoV-2 ORF3A-mediated autophagy but is unlikely to be required for ORF3A-mediated induction of reticulophagy. Heme oxygenase 1 soluble form. Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron.
Alternative names
HO, HO1, HMOX1, Heme oxygenase 1, HO-1
Recommended products
Recombinant Human Heme Oxygenase 1 protein is a Human Full Length protein, in the 1 to 288 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, WB.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, WB
- Biologically active
- No
- Accession
- P09601-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00174% PMSF
Sequence info
Amino acid sequence
- M E R P Q P D S M P Q D L S E A L K E A T K E V H T Q A E N A E F M R N F Q K G Q V T R D G F K L V M A S L Y H I Y V A L E E E I E R N K E S P V F A P V Y F P E E L H R K A A L E Q D L A F W Y G P R W Q E V I P Y T P A M Q R Y V K R L H E V G R T E P E L L V A H A Y T R Y L G D L S G G Q V L K K I A Q K A L D L P S S G E G L A F F T F P N I A S A T K F K Q L Y R S R M N S L E M T P A V R Q R V I E E A K T A F L L N I Q L F E E L Q E L L T H D T K D Q S P S R A P G L R Q R A S N K V Q D S A P V E T P R G K P P L N T R S Q A P L L R W V L T L S F L V A T V A V G L Y A M
- Accession
- P09601
- Protein length
- Full Length
- Predicted molecular weight
- 56 kDa
- Amino acids
- 1 to 288
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Purity was determined to be >95% by densitometry.
General info
- Function
Heme oxygenase 1
- Sequence similarities
Belongs to the heme oxygenase family.
- Post-translational modifications
A soluble form arises by proteolytic removal of the membrane anchor.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Heme Oxygenase 1 also known as HO-1 or HMOX1 is an enzyme that plays an important mechanistic role in heme catabolism. It catalyzes the degradation of heme into biliverdin carbon monoxide and free iron. This process involves the cleavage of the heme ring. HO-1 has a molecular weight of approximately 32 kDa. It is widely expressed in numerous tissues but is especially abundant in the liver and spleen. Its expression is induced by heme and other stress stimuli such as heavy metals cytokines and reactive oxygen species.
- Biological function summary
Heme Oxygenase 1 serves important protective functions in the body. It is not part of a larger complex but its products such as carbon monoxide and biliverdin have their own biological activities. Carbon monoxide produced by HO-1 has antiflammatory properties and can modulate apoptotic pathways. Biliverdin is reduced to bilirubin which acts as an antioxidant. The enzyme therefore directly influences cellular stress responses and maintains cellular homeostasis through these processes.
- Pathways
Heme Oxygenase 1 is integrally involved in oxidative stress response and heme metabolism. It participates in the cellular response to oxidative damage by reducing oxidative stress and promoting cytoprotection. Through its heme degradation activity it is connected with the synthesis of biologically active molecules like bilirubin and carbon monoxide. Heme Oxygenase 1 activity is related to other proteins in oxidative stress pathways such as Nuclear Factor Erythroid 2-Related Factor 2 (Nrf2) which regulates its expression and globins which are sources of heme for HO-1 activity.
- Associated diseases and disorders
Heme Oxygenase 1 has been linked to conditions like cardiovascular diseases and neurodegenerative disorders. Its expression can attenuate the severity of atherosclerosis where oxidative stress is an important factor. In neurodegenerative diseases HO-1’s antioxidant properties may provide neuroprotection by mitigating oxidative damage. The protein's interactions with inflammatory cytokines such as Interleukin-6 and tumor necrosis factor-alpha influence its activity in these disease contexts.
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2 product images
SDS-PAGE - Recombinant Human Heme Oxygenase 1 protein (ab85243)
SDS-PAGE showing ab85243 at approximately 56kDa.
Sandwich ELISA - Recombinant Human Heme Oxygenase 1 protein (ab85243)
Standard Curve for Heme Oxygenase 1 (Analyte: Heme Oxygenase 1 protein (Tagged) (ab85243));
dilution range 1pg/ml to 1μg/ml using Capture Antibody Mouse monoclonal [HO-1-1] to Heme
Oxygenase 1 (Anti-Heme Oxygenase 1 antibody [HO-1-1] ab13248) at 5μg/ml and Detector Antibody Rabbit polyclonal to Heme Oxygenase 1
(Anti-Heme Oxygenase 1 antibody ab13243) at 0.5μg/ml.
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