Recombinant Human HEMK2/N6AMT1 protein
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Recombinant Human HEMK2/N6AMT1 protein is a Human Full Length protein, in the 1 to 214 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE, Mass Spec.
View Alternative Names
C21orf127, HEMK2, KMT9, PRED28, N6AMT1, Methyltransferase N6AMT1, HemK methyltransferase family member 2, Lysine N-methyltransferase 9, Methylarsonite methyltransferase N6AMT1, Protein N(5)-glutamine methyltransferase, M.HsaHemK2P
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant Human HEMK2/N6AMT1 protein (AB187466)
15% SDS-PAGE analysis of ab187466 (3μg).
Reactivity data
Product details
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
HEMK2/N6AMT1 involves the methylation of specific proteins contributing to protein modifications and cell function regulation. This protein often forms part of a larger complex that facilitates its role in transferring methyl groups onto target proteins which in turn affects their function and interactions. HEMK2/N6AMT1's activity influences processes like translation fidelity by targeting elongation factors for methylation helping maintain cellular homeostasis.
Pathways
HEMK2/N6AMT1 plays a role in epigenetic regulation and protein modifications. It participates in the methylation pathway integrating with processes that impact gene expression and protein interactions. In these pathways HEMK2/N6AMT1 interacts with related proteins such as TRMT112 which acts as an activator and stabilizer. These pathways are important for the control of gene expression showcasing the broad influence HEMK2/N6AMT1 has on cellular functioning by regulating the methylation status of proteins.
Specifications
Form
Liquid
Additional notes
ab187466 is purified using anion-exchange chromatography (DEAE sepharose resin) and gel-filtration chromatography (Sephacryl S-200) with 20 mM Tris pH 7.5, 2 mM EDTA.
General info
Function
Methyltransferase that can methylate proteins and, to a lower extent, arsenic (PubMed : 18539146, PubMed : 21193388, PubMed : 30017583, PubMed : 31061526, PubMed : 31636962). Catalytic subunit of a heterodimer with TRMT112, which monomethylates 'Lys-12' of histone H4 (H4K12me1), a modification present at the promoters of numerous genes encoding cell cycle regulators (PubMed : 31061526). Catalytic subunit of a heterodimer with TRMT112, which catalyzes N5-methylation of Glu residue of proteins with a Gly-Gln-Xaa-Xaa-Xaa-Arg motif (PubMed : 18539146, PubMed : 31632689, PubMed : 31636962). Methylates ETF1 on 'Gln-185'; ETF1 needs to be complexed to ERF3 in its GTP-bound form to be efficiently methylated (PubMed : 18539146, PubMed : 20606008, PubMed : 31061526, PubMed : 31636962). May also play a role in the modulation of arsenic-induced toxicity by mediating the conversion of monomethylarsonous acid (3+) into the less toxic dimethylarsonic acid (PubMed : 21193388, PubMed : 25997655). It however only plays a limited role in arsenic metabolism compared with AS3MT (PubMed : 25997655).
Sequence similarities
Belongs to the eukaryotic/archaeal PrmC-related family.
Post-translational modifications
Isoform 1. Ubiquitinated, leading to its degradation by the proteasome.. Isoform 2. Ubiquitinated, leading to its degradation by the proteasome.
Subcellular localisation
Nucleus
Target data
Complementary Products
Product promise
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