Recombinant Human Hemoglobin subunit alpha protein (denatured) is a Human Full Length protein, in the 1 to 142 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- DDDDK tag N-Terminus His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M R G S H H H H H H G M A S M T G G Q Q M G R D L Y D D D D K D R W G S H M V L S P A D K T N V K A A W G K V G A H A G E Y G A E A L E R M F L S F P T T K T Y F P H F D L S H G S A Q V K G H G K K V A D A L T N A V A H V D D M P N A L S A L S D L H A H K L R V D P V N F K L L S H C L L V T L A A H L P A E F T P A V H A S L D K F L A S V S T V L T S K Y R
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Involved in oxygen transport from the lung to the various peripheral tissues. Hemopressin. Hemopressin acts as an antagonist peptide of the cannabinoid receptor CNR1 (PubMed:18077343). Hemopressin-binding efficiently blocks cannabinoid receptor CNR1 and subsequent signaling (PubMed:18077343).
Alternative names
HBA2, HBA1, Hemoglobin subunit alpha, Alpha-globin, Hemoglobin alpha chain
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Recombinant Human Hemoglobin subunit alpha protein (denatured) is a Human Full Length protein, in the 1 to 142 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- DDDDK tag N-Terminus His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- P69905-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 12.01% Urea, 0.58% Sodium chloride, 0.32% Tris HCl, 0.03% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M R G S H H H H H H G M A S M T G G Q Q M G R D L Y D D D D K D R W G S H M V L S P A D K T N V K A A W G K V G A H A G E Y G A E A L E R M F L S F P T T K T Y F P H F D L S H G S A Q V K G H G K K V A D A L T N A V A H V D D M P N A L S A L S D L H A H K L R V D P V N F K L L S H C L L V T L A A H L P A E F T P A V H A S L D K F L A S V S T V L T S K Y R
- Accession
- P69905
- Protein length
- Full Length
- Predicted molecular weight
- 19.5 kDa
- Amino acids
- 1 to 142
- Nature
- Recombinant
- Tags
- DDDDK tag N-Terminus, His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab131697 was purified using conventional chromatography techniques.
General info
- Function
Involved in oxygen transport from the lung to the various peripheral tissues.
- Sequence similarities
Belongs to the globin family.
- Post-translational modifications
The initiator Met is not cleaved in variant Thionville and is acetylated.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Hemoglobin subunit alpha also known as alpha-globin is a component of the hemoglobin protein complex which plays an important role in oxygen transport within the blood. Alpha-globin has an approximate molecular weight of 15.1 kDa and is expressed highly in the red blood cells. It is part of the hemoglobin tetramer along with two beta subunits each one containing an iron-bound heme group. Variants of the alpha chain can be studied using hemoglobin peptides or denatured hemoglobin samples. Researchers can further analyze the alpha hemoglobin using methods like SDS-PAGE or alpha ELISA assays.
- Biological function summary
Hemoglobin subunit alpha forms an important part of the hemoglobin complex facilitating the binding and release of oxygen molecules as blood circulates in the body. Alpha hemoglobin ensures efficient loading of oxygen in the lungs and unloading in tissues maintaining cellular respiration. The subunit plays a structural role as well stabilizing the hemoglobin tetramer for optimal function. Its ability to carry oxygen depends on the cooperative interaction between its alpha and beta globin counterparts.
- Pathways
Hemoglobin subunit alpha operates predominantly within the oxygen transport pathway which is essential to meet the metabolic demands of cells. It also links to pathways involving iron metabolism given its coordination with heme groups. Alpha hemoglobin interacts cooperatively with proteins such as beta-globin to ensure efficient oxygen delivery. This interplay is highlighted when examining hemoglobin biosynthesis and breakdown pathways.
- Associated diseases and disorders
Alpha-globin is linked to conditions like alpha-thalassemia and sickle cell disease. These disorders result from mutations in the hemoglobin alpha or beta subunits leading to imbalanced globin production or abnormal hemoglobin structures. Alpha thalassemia is connected with unequal production of globin chains affecting hemoglobin stability while beta-globin mutations lead to sickle cell disease with altered oxygen delivery. Anti-hemoglobin antibodies might help in researching these conditions allowing a better understanding of molecular changes and potential therapeutic targets.
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3 product images
Western blot - Recombinant Human Hemoglobin subunit alpha protein (denatured) (ab131697)
**Blocking/Dilution buffer:** 5% NFDM/TBST.
All lanes: Western blot - Anti-Hemoglobin subunit beta antibody [EPR20614] (Anti-Hemoglobin subunit beta antibody [EPR20614] ab214049) at 1/1000 dilution
Lane 1: Western blot - Recombinant Human Hemoglobin subunit alpha protein (denatured) (ab131697) at 0.01 µg
Lane 2: His-tagged human Hemoglobin subunit beta/ba1 (aa3-147) recombinant protein at 0.01 µg
Lane 3: His-tagged human Hemoglobin subunit delta (aa3-147) recombinant protein at 0.01 µg
Lane 4: His-tagged human Hemoglobin subunit epsilon (aa3-147) recombinant protein at 0.01 µg
Lane 5: His-tagged human Hemoglobin subunit Gamma-2 (aa2-147) recombinant protein at 0.01 µg
Lane 6: His-tagged human Hemoglobin subunit Gamma-1 (aa2-147) recombinant protein at 0.01 µg
Lane 7: Western blot - Recombinant Human Hemoglobin subunit zeta protein (Recombinant Human Hemoglobin subunit zeta protein ab95347) at 0.01 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 16 kDa
Observed band size: 15 kDa
Exposure time: 3s
Western blot - Recombinant Human Hemoglobin subunit alpha protein (denatured) (ab131697)
Blocking/Dilutiing buffer and concentration: 5% NFDM/TBST
Exposure time: 100 secondsAll lanes: Western blot - Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)] (Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)] ab131225) at 1/200 dilution
Lane 1: Western blot - Recombinant Human Hemoglobin subunit alpha protein (denatured) (ab131697) at 0.1 µg
Lane 2: His-tagged human Hemoglobin subunit beta/ba1 (aa 3-147) recombinant protein at 0.1 µg
Lane 3: His-tagged human Hemoglobin subunit delta (aa 3-147) recombinant protein at 0.1 µg
Lane 4: His-tagged full length human Hemoglobin subunit delta recombinant protein at 0.1 µg
Lane 5: His-tagged human Hemoglobin subunit epsilon (aa 3-147) recombinant protein at 0.1 µg
Lane 6: His-tagged human Hemoglobin subunit gamma-2 (aa 2-147) recombinant protein at 0.1 µg
Lane 7: His-tagged human Hemoglobin subunit gamma-1 (aa 2-147) recombinant protein at 0.1 µg
Lane 8: Western blot - Recombinant Human Hemoglobin subunit zeta protein (Recombinant Human Hemoglobin subunit zeta protein ab95347) at 0.1 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
SDS-PAGE - Recombinant Human Hemoglobin subunit alpha protein (denatured) (ab131697)
SDS-PAGE analysis of ab131697 (3 μg) under reducing conditions and visualized by coomassie blue stain.
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