Recombinant Human Hemoglobin subunit beta protein is a Human Fragment protein, in the 38 to 147 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Images
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
Amino acid sequence
W T Q R F F E S F G D L S T P D A V M G N P K V K A H G K K V L G A F S D G L A H L D N L K G T F A T L S E L H C D K L H V D P E N F R L L G N V L V C V L A H H F G K E F T P P V Q A A Y Q K V V A G V A N A L P H K Y H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Involved in oxygen transport from the lung to the various peripheral tissues. LVV-hemorphin-7 potentiates the activity of bradykinin, causing a decrease in blood pressure. Spinorphin. Functions as an endogenous inhibitor of enkephalin-degrading enzymes such as DPP3, and as a selective antagonist of the P2RX3 receptor which is involved in pain signaling, these properties implicate it as a regulator of pain and inflammation.
Alternative names
Hemoglobin subunit beta, Beta-globin, Hemoglobin beta chain, HBB
Recommended products
Recombinant Human Hemoglobin subunit beta protein is a Human Fragment protein, in the 38 to 147 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
- Accession
- P68871-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- W T Q R F F E S F G D L S T P D A V M G N P K V K A H G K K V L G A F S D G L A H L D N L K G T F A T L S E L H C D K L H V D P E N F R L L G N V L V C V L A H H F G K E F T P P V Q A A Y Q K V V A G V A N A L P H K Y H
- Accession
- P68871
- Protein length
- Fragment
- Amino acids
- 38 to 147
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Involved in oxygen transport from the lung to the various peripheral tissues.
- Sequence similarities
Belongs to the globin family.
- Post-translational modifications
Glucose reacts non-enzymatically with the N-terminus of the beta chain to form a stable ketoamine linkage. This takes place slowly and continuously throughout the 120-day life span of the red blood cell. The rate of glycation is increased in patients with diabetes mellitus.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as Hemoglobin subunit beta.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The hemoglobin subunit beta also known as beta hemoglobin or hemoglobin beta subunit is a component of the larger hemoglobin molecule important for transporting oxygen in red blood cells. This protein has a molecular weight of approximately 16 kDa. Hemoglobin subunit beta joins with alpha hemoglobin subunit to form hemoglobin A the most common form in adult humans. Expression of this protein happens in the erythroid cells of the bone marrow where it plays an important role in the production of hemoglobin during red blood cell formation.
- Biological function summary
The hemoglobin beta subunit is essential for binding and releasing oxygen to tissues throughout the body. As part of the hemoglobin complex it helps stabilize the oxygen binding affinity through cooperative interactions with the alpha subunits. When oxygen binds to the heme groups in these subunits a conformational change occurs enhancing the efficiency of oxygen transport. This dynamic action allows hemoglobin to carry oxygen from the lungs to tissues and return carbon dioxide for expulsion.
- Pathways
Hemoglobin beta subunit significantly participates in the oxygen transport and carbon dioxide exchange pathways. It interacts closely with carbonic anhydrase in the process of carbon dioxide transport converting it to bicarbonate for efficient clearance from the body. In this capacity the protein stands as a central component of the erythrocyte function and blood gas transport system partnering physiologically with proteins such as cytochrome b5 reductase in electron transfer-related pathways.
- Associated diseases and disorders
Hemoglobin subunit beta is prominently associated with hemoglobinopathies like sickle cell disease and beta-thalassemia. Mutations in its gene can lead to abnormal hemoglobin function or structure causing red blood cell disorders that impact oxygen delivery. In sickle cell disease an amino acid substitution causes hemoglobin to polymerize under low oxygen resulting in sickle-shaped cells. In beta-thalassemia reduced or absent beta-globin chain production leads to anemia. These hemoglobin-related disorders are tightly connected to genetic mutations influencing other proteins such as alpha-globin complicating the clinical picture.
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1 product image
SDS-PAGE - Recombinant Human Hemoglobin subunit beta protein (ab158639)
ab158639 on a 12.5% SDS-PAGE stained with Coomassie Blue.
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