Recombinant Human Hemoglobin subunit gamma 2 protein is a Human Full Length protein, in the 1 to 147 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M G H F T E E D K A T I T S L W G K V N V E D A G G E T L G R L L V V Y P W T Q R F F D S F G N L S S A S A I M G N P K V K A H G K K V L T S L G D A I K H L D D L K G T F A Q L S E L H C D K L H V D P E N F K L L G N V L V T V L A I H F G K E F T P E V Q A S W Q K M V T G V A S A L S S R Y H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Gamma chains make up the fetal hemoglobin F, in combination with alpha chains.
Alternative names
Hemoglobin subunit gamma-2, Gamma-2-globin, Hb F Ggamma, Hemoglobin gamma-2 chain, Hemoglobin gamma-G chain, HBG2
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Recombinant Human Hemoglobin subunit gamma 2 protein is a Human Full Length protein, in the 1 to 147 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- P69892-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M G H F T E E D K A T I T S L W G K V N V E D A G G E T L G R L L V V Y P W T Q R F F D S F G N L S S A S A I M G N P K V K A H G K K V L T S L G D A I K H L D D L K G T F A Q L S E L H C D K L H V D P E N F K L L G N V L V T V L A I H F G K E F T P E V Q A S W Q K M V T G V A S A L S S R Y H
- Accession
- P69892
- Protein length
- Full Length
- Predicted molecular weight
- 18.5 kDa
- Amino acids
- 1 to 147
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab139782 is purified using conventional chromatography techniques.
General info
- Function
Gamma chains make up the fetal hemoglobin F, in combination with alpha chains.
- Sequence similarities
Belongs to the globin family.
- Post-translational modifications
Acetylation of Gly-2 converts Hb F to the minor Hb F1.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Hemoglobin subunit gamma 2 also called gamma globin or HBG2 is a component of fetal hemoglobin (HbF). It has a molecular mass of approximately 16 kDa. The gamma chains specifically gamma 2 replace the beta chains in adult hemoglobin creating an embryonic and fetal form known as hemoglobin gamma. This subunit is expressed in fetal red blood cells and becomes virtually undetectable shortly after birth as the hemoglobin switches from fetal (HbF) to adult forms (HbA).
- Biological function summary
Hemoglobin subunit gamma 2 plays an important role in oxygen transport during fetal development forming fetal hemoglobin by pairing with alpha globin chains. This allows hemoglobin to capture oxygen from maternal blood efficiently. In this complex the gamma subunit increases oxygen affinity an adaptation important for intrauterine life. Its increased presence in early development is necessary until it decreases as adult-type beta globin becomes the primary subunit in hemoglobin.
- Pathways
Hemoglobin subunit gamma 2 is integral to the regulation of hemoglobin synthesis participating in the globin gene expression pathway. It associates with proteins like alpha globin and beta globin. This subunit is significant in the hemoglobin switching process where fetal hemoglobin transitions to adult hemoglobin a critical aspect of maturation after birth.
- Associated diseases and disorders
Hemoglobin subunit gamma 2 is often linked to hemoglobinopathies such as beta-thalassemia and sickle cell disease. In these conditions gamma globin reactivation can compensate for defective hemoglobin improving clinical outcomes. This connection to disorders highlights potential therapeutic strategies where increasing gamma globin expression could alleviate symptoms by complementing defective beta globin. Exploring this target further may yield insights into treating hemoglobin-related conditions more effectively.
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1 product image
SDS-PAGE - Recombinant Human Hemoglobin subunit gamma 2 protein (ab139782)
15% SDS-PAGE analysis of ab139782 (3μg).
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