Recombinant Human Hemoglobin subunit zeta protein is a Human Full Length protein, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
>90% SDS-PAGE
Escherichia coli
Tag free
SDS-PAGE, MS
No
M S L T K T E R T I I V S M W A K I S T Q A D T I G T E T L E R L F L S H P Q T K T Y F P H F D L H P G S A Q L R A H G S K V V A A V G D A V K S I D D I G G A L S K L S E L H A Y I L R V D P V N F K L L S H C L L V T L A A R F P A D F T A E A H A A W D K F L S V V S S V L T E K Y R L E H H H H H H
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
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The zeta chain is an alpha-type chain of mammalian embryonic hemoglobin.
HBZ2, HBZ2, HBZ, Hemoglobin subunit zeta, HBAZ, Hemoglobin zeta chain, Zeta-globin
Recombinant Human Hemoglobin subunit zeta protein is a Human Full Length protein, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
>90% SDS-PAGE
Escherichia coli
Tag free
SDS-PAGE, MS
No
No
Human
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
M S L T K T E R T I I V S M W A K I S T Q A D T I G T E T L E R L F L S H P Q T K T Y F P H F D L H P G S A Q L R A H G S K V V A A V G D A V K S I D D I G G A L S K L S E L H A Y I L R V D P V N F K L L S H C L L V T L A A R F P A D F T A E A H A A W D K F L S V V S S V L T E K Y R L E H H H H H H
Full Length
Recombinant
Liquid
ab95347 is purified using conventional chromatography techniques.
The zeta chain is an alpha-type chain of mammalian embryonic hemoglobin.
Belongs to the globin family.
Blue Ice
-20°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
This supplementary information is collated from multiple sources and compiled automatically.
The Hemoglobin subunit zeta also known as HBZ or ζ-globin is a protein subunit of hemoglobin present predominantly during early embryonic stages. It is a part of the alpha-globin gene cluster located on chromosome 16. This protein typically measures approximately 16 kDa in molecular weight. Expression of the zeta subunit is mostly confined to embryonic tissues including the yolk sac and early erythroid cells. Throughout development the presence of HBZ is significantly reduced as other globin genes take precedence for fetal and adult hemoglobin production.
Hemoglobin subunit zeta plays a role in the formation of embryonic hemoglobin specifically the ζ2ε2 or Gower-1 hemoglobin complex. This complex facilitates the transfer of oxygen from the mother to the developing embryo. The zeta subunit interacts with other globin genes to form early hemoglobin types critical for oxygen transport at this stage. It enables the initial establishment of a functional circulatory system during early development.
The Hemoglobin subunit zeta engages in the oxygen transport pathway important to the early stages of embryogenesis. As part of this pathway it directly interacts with epsilon-globin to form the embryonic Gower-1 hemoglobin. This pathway operates in parallel with but distinctly from the fetal hemoglobin pathway involving alpha and gamma globins. These interactions illustrate the complex switch and regulation of globin gene expression that occurs as development progresses.
Hemoglobin subunit zeta has associations with hemoglobinopathies such as alpha-thalassemia. Mutations or deletions in the alpha-globin gene cluster can impact HBZ production contributing to anemia and related disorders. Additionally its dysregulation can be a marker for developmental delays in hematopoiesis. Interactions with the alpha-globin proteins highlight the clinical relevance of studying HBZ mutations and their impacts on blood disorders.
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**Blocking/Dilution buffer:** 5% NFDM/TBST.
All lanes: Western blot - Anti-Hemoglobin subunit beta antibody [EPR20614] (Anti-Hemoglobin subunit beta antibody [EPR20614] ab214049) at 1/1000 dilution
Lane 1: Western blot - Recombinant Human Hemoglobin subunit alpha protein (denatured) (Recombinant Human Hemoglobin subunit alpha protein (denatured) ab131697) at 0.01 µg
Lane 2: His-tagged human Hemoglobin subunit beta/ba1 (aa3-147) recombinant protein at 0.01 µg
Lane 3: His-tagged human Hemoglobin subunit delta (aa3-147) recombinant protein at 0.01 µg
Lane 4: His-tagged human Hemoglobin subunit epsilon (aa3-147) recombinant protein at 0.01 µg
Lane 5: His-tagged human Hemoglobin subunit Gamma-2 (aa2-147) recombinant protein at 0.01 µg
Lane 6: His-tagged human Hemoglobin subunit Gamma-1 (aa2-147) recombinant protein at 0.01 µg
Lane 7: Western blot - Recombinant Human Hemoglobin subunit zeta protein (ab95347) at 0.01 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 16 kDa
Observed band size: 15 kDa
Exposure time: 3s
Blocking/Dilution buffer: 5% NFDM/TBST.
Blocking/Dilutiing buffer and concentration: 5% NFDM/TBST
Exposure time: 100 seconds
All lanes: Western blot - Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)] (Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)] ab131225) at 1/200 dilution
Lane 1: Western blot - Recombinant Human Hemoglobin subunit alpha protein (denatured) (Recombinant Human Hemoglobin subunit alpha protein (denatured) ab131697) at 0.1 µg
Lane 2: His-tagged human Hemoglobin subunit beta/ba1 (aa 3-147) recombinant protein at 0.1 µg
Lane 3: His-tagged human Hemoglobin subunit delta (aa 3-147) recombinant protein at 0.1 µg
Lane 4: His-tagged full length human Hemoglobin subunit delta recombinant protein at 0.1 µg
Lane 5: His-tagged human Hemoglobin subunit epsilon (aa 3-147) recombinant protein at 0.1 µg
Lane 6: His-tagged human Hemoglobin subunit gamma-2 (aa 2-147) recombinant protein at 0.1 µg
Lane 7: His-tagged human Hemoglobin subunit gamma-1 (aa 2-147) recombinant protein at 0.1 µg
Lane 8: Western blot - Recombinant Human Hemoglobin subunit zeta protein (ab95347) at 0.1 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Blocking/Dilutiing buffer and concentration: 5% NFDM/TBST
Exposure time: 100 seconds
15% SDS-PAGE analysis of 3μg ab95347.
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