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Recombinant Human Hemoglobin subunit zeta protein is a Human Full Length protein, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.

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Images

Western blot - Recombinant Human Hemoglobin subunit zeta protein (AB95347), expandable thumbnail
  • Western blot - Recombinant Human Hemoglobin subunit zeta protein (AB95347), expandable thumbnail
  • SDS-PAGE - Recombinant Human Hemoglobin subunit zeta protein (AB95347), expandable thumbnail

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

Tag free

Applications

SDS-PAGE, MS

Biologically active

No

Amino acid sequence

M S L T K T E R T I I V S M W A K I S T Q A D T I G T E T L E R L F L S H P Q T K T Y F P H F D L H P G S A Q L R A H G S K V V A A V G D A V K S I D D I G G A L S K L S E L H A Y I L R V D P V N F K L L S H C L L V T L A A R F P A D F T A E A H A A W D K F L S V V S S V L T E K Y R L E H H H H H H

Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Application

MS

Reactivity

Reacts

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

The zeta chain is an alpha-type chain of mammalian embryonic hemoglobin.

Alternative names

Recommended products

Recombinant Human Hemoglobin subunit zeta protein is a Human Full Length protein, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Applications

SDS-PAGE, MS

Accession
P02008-1
Animal free

No

Species

Human

Concentration
Loading...
Storage buffer

pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

Sequence info

Amino acid sequence

M S L T K T E R T I I V S M W A K I S T Q A D T I G T E T L E R L F L S H P Q T K T Y F P H F D L H P G S A Q L R A H G S K V V A A V G D A V K S I D D I G G A L S K L S E L H A Y I L R V D P V N F K L L S H C L L V T L A A R F P A D F T A E A H A A W D K F L S V V S S V L T E K Y R L E H H H H H H

Accession

P02008

Protein length

Full Length

Nature

Recombinant

Specifications

Form

Liquid

Additional notes

ab95347 is purified using conventional chromatography techniques.

General info

Function

The zeta chain is an alpha-type chain of mammalian embryonic hemoglobin.

Sequence similarities

Belongs to the globin family.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The Hemoglobin subunit zeta also known as HBZ or ζ-globin is a protein subunit of hemoglobin present predominantly during early embryonic stages. It is a part of the alpha-globin gene cluster located on chromosome 16. This protein typically measures approximately 16 kDa in molecular weight. Expression of the zeta subunit is mostly confined to embryonic tissues including the yolk sac and early erythroid cells. Throughout development the presence of HBZ is significantly reduced as other globin genes take precedence for fetal and adult hemoglobin production.

Biological function summary

Hemoglobin subunit zeta plays a role in the formation of embryonic hemoglobin specifically the ζ2ε2 or Gower-1 hemoglobin complex. This complex facilitates the transfer of oxygen from the mother to the developing embryo. The zeta subunit interacts with other globin genes to form early hemoglobin types critical for oxygen transport at this stage. It enables the initial establishment of a functional circulatory system during early development.

Pathways

The Hemoglobin subunit zeta engages in the oxygen transport pathway important to the early stages of embryogenesis. As part of this pathway it directly interacts with epsilon-globin to form the embryonic Gower-1 hemoglobin. This pathway operates in parallel with but distinctly from the fetal hemoglobin pathway involving alpha and gamma globins. These interactions illustrate the complex switch and regulation of globin gene expression that occurs as development progresses.

Associated diseases and disorders

Hemoglobin subunit zeta has associations with hemoglobinopathies such as alpha-thalassemia. Mutations or deletions in the alpha-globin gene cluster can impact HBZ production contributing to anemia and related disorders. Additionally its dysregulation can be a marker for developmental delays in hematopoiesis. Interactions with the alpha-globin proteins highlight the clinical relevance of studying HBZ mutations and their impacts on blood disorders.

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3 product images

  • Western blot - Recombinant Human Hemoglobin subunit zeta protein (ab95347), expandable thumbnail

    Western blot - Recombinant Human Hemoglobin subunit zeta protein (ab95347)

    **Blocking/Dilution buffer:** 5% NFDM/TBST.

    All lanes: Western blot - Anti-Hemoglobin subunit beta antibody [EPR20614] (Anti-Hemoglobin subunit beta antibody [EPR20614] ab214049) at 1/1000 dilution

    Lane 1: Western blot - Recombinant Human Hemoglobin subunit alpha protein (denatured) (Recombinant Human Hemoglobin subunit alpha protein (denatured) ab131697) at 0.01 µg

    Lane 2: His-tagged human Hemoglobin subunit beta/ba1 (aa3-147) recombinant protein at 0.01 µg

    Lane 3: His-tagged human Hemoglobin subunit delta (aa3-147) recombinant protein at 0.01 µg

    Lane 4: His-tagged human Hemoglobin subunit epsilon (aa3-147) recombinant protein at 0.01 µg

    Lane 5: His-tagged human Hemoglobin subunit Gamma-2 (aa2-147) recombinant protein at 0.01 µg

    Lane 6: His-tagged human Hemoglobin subunit Gamma-1 (aa2-147) recombinant protein at 0.01 µg

    Lane 7: Western blot - Recombinant Human Hemoglobin subunit zeta protein (ab95347) at 0.01 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 16 kDa

    Observed band size: 15 kDa

    Exposure time: 3s

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Recombinant Human Hemoglobin subunit zeta protein (ab95347), expandable thumbnail

    Western blot - Recombinant Human Hemoglobin subunit zeta protein (ab95347)

    Blocking/Dilutiing buffer and concentration: 5% NFDM/TBST
    Exposure time: 100 seconds

    All lanes: Western blot - Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)] (Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)] ab131225) at 1/200 dilution

    Lane 1: Western blot - Recombinant Human Hemoglobin subunit alpha protein (denatured) (Recombinant Human Hemoglobin subunit alpha protein (denatured) ab131697) at 0.1 µg

    Lane 2: His-tagged human Hemoglobin subunit beta/ba1 (aa 3-147) recombinant protein at 0.1 µg

    Lane 3: His-tagged human Hemoglobin subunit delta (aa 3-147) recombinant protein at 0.1 µg

    Lane 4: His-tagged full length human Hemoglobin subunit delta recombinant protein at 0.1 µg

    Lane 5: His-tagged human Hemoglobin subunit epsilon (aa 3-147) recombinant protein at 0.1 µg

    Lane 6: His-tagged human Hemoglobin subunit gamma-2 (aa 2-147) recombinant protein at 0.1 µg

    Lane 7: His-tagged human Hemoglobin subunit gamma-1 (aa 2-147) recombinant protein at 0.1 µg

    Lane 8: Western blot - Recombinant Human Hemoglobin subunit zeta protein (ab95347) at 0.1 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Blocking/Dilutiing buffer and concentration: 5% NFDM/TBST

    Exposure time: 100 seconds

  • SDS-PAGE - Recombinant Human Hemoglobin subunit zeta protein (ab95347), expandable thumbnail

    SDS-PAGE - Recombinant Human Hemoglobin subunit zeta protein (ab95347)

    15% SDS-PAGE analysis of 3μg ab95347.

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Product protocols

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