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AB181927

Recombinant Human Heparanase 1 protein (denatured) (His tag N-Terminus)

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Recombinant Human Heparanase 1 protein (denatured) (His tag N-Terminus) is a Human Full Length protein, in the 36 to 543 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

View Alternative Names

HEP, HPA, HPA1, HPR1, HPSE1, HSE1, HPSE, Heparanase, Endo-glucoronidase, Heparanase-1, Hpa1

1 Images
SDS-PAGE - Recombinant Human Heparanase 1 protein (denatured) (His tag N-Terminus) (AB181927)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Heparanase 1 protein (denatured) (His tag N-Terminus) (AB181927)

15% SDS-PAGE analysis of ab181927 (3μg)

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q9Y251

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSQDVVDLDFFTQEPLHLVSPSFLSVTIDANLATDPRFLILLGSPKLRTLARGLSPAYLRFGGTKTDFLIFDPKKESTFEERSYWQSQVNQDICKYGSIPPDVEEKLRLEWPYQEQLLLREHYQKKFKNSTYSRSSVDVLYTFANCSGLDLIFGLNALLRTADLQWNSSNAQLLLDYCSSKGYNISWELGNEPNSFLKKADIFINGSQLGEDFIQLHKLLRKSTFKNAKLYGPDVGQPRRKTAKMLKSFLKAGGEVIDSVTWHHYYLNGRTATKEDFLNPDVLDIFISSVQKVFQVVESTRPGKKVWLGETSSAYGGGAPLLSDTFAAGFMWLDKLGLSARMGIEVVMRQVFFGAGNYHLVDENFDPLPDYWLSLLFKKLVGTKVLMASVQGSKRRKLRVYLHCTNTDNPRYKEGDLTLYAINLHNVTKYLRLPYPFSNKQVDKYLLRPLGPHGLLSKSVQLNGLTLKMVDDQTLPPLMEKPLRPGSSLGLPAFSYSFFVIRNAKVAACI","proteinLength":"Full Length","predictedMolecularWeight":"60 kDa","actualMolecularWeight":null,"aminoAcidEnd":543,"aminoAcidStart":36,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9Y251","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Heparanase 1 also known as HPA1 or HPSE is an endo-β-D-glucuronidase enzyme with a molecular mass of approximately 50 kDa. It functions mechanically by cleaving heparan sulfate (HS) chains which are long sugar chains found on the extracellular matrix and cell surfaces. This cleavage activity remodels the matrix and influences cell behavior. Heparanase 1 is mainly expressed in the placenta lymphoid tissues and various tumor cells where it plays a role in matrix degradation and cell migration.
Biological function summary

When heparan sulfate is cleaved by Heparanase 1 it causes the release of HS-bound growth factors and cytokines making them available to cells. This enzymatic activity facilitates cell proliferation and angiogenesis affecting processes important for tissue repair and cancer progression. Heparanase 1 does not require a complex for its function but operates as a monomeric enzyme. Its activity is investigated using assays like heparanase activity assays or ELISA for measuring the enzyme's activity in different biological contexts including mouse models.

Pathways

The activity of Heparanase 1 intersects significantly with the regulation of angiogenesis and cell signaling pathways such as the VEGF and FGF pathways. These pathways are important for new blood vessel formation making them significant in both normal development and cancer. Heparanase 1 interacts with proteins such as VEGF which is important for blood vessel growth and integrins which are involved in cell-matrix interactions and signaling.

Heparanase 1 is associated with tumor metastasis and inflammation. Its ability to degrade the extracellular matrix enables cancer cell invasion and metastasis. The enzyme also plays a role in inflammatory diseases where it modulates the immune response. Through these disease pathways it relates to other proteins such as matrix metalloproteinases (MMPs) which also participate in matrix remodeling during tumor progression and inflammation.
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Specifications

Form

Liquid

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General info

Function

Endoglycosidase that cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans. Participates in extracellular matrix (ECM) degradation and remodeling. Selectively cleaves the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying either a 3-O-sulfo or a 6-O-sulfo group. Can also cleave the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying a 2-O-sulfo group, but not linkages between a glucuronic acid unit and a 2-O-sulfated iduronic acid moiety. It is essentially inactive at neutral pH but becomes active under acidic conditions such as during tumor invasion and in inflammatory processes. Facilitates cell migration associated with metastasis, wound healing and inflammation. Enhances shedding of syndecans, and increases endothelial invasion and angiogenesis in myelomas. Acts as a procoagulant by increasing the generation of activation factor X in the presence of tissue factor and activation factor VII. Increases cell adhesion to the extracellular matrix (ECM), independent of its enzymatic activity. Induces AKT1/PKB phosphorylation via lipid rafts increasing cell mobility and invasion. Heparin increases this AKT1/PKB activation. Regulates osteogenesis. Enhances angiogenesis through up-regulation of SRC-mediated activation of VEGF. Implicated in hair follicle inner root sheath differentiation and hair homeostasis.

Sequence similarities

Belongs to the glycosyl hydrolase 79 family.

Post-translational modifications

Proteolytically processed. The cleavage of the 65 kDa form leads to the generation of a linker peptide, and 8 kDa and 50 kDa products. The active form, the 8/50 kDa heterodimer, is resistant to degradation. Complete removal of the linker peptide appears to be a prerequisite to the complete activation of the enzyme.. N-glycosylated. Glycosylation of the 50 kDa subunit appears to be essential for its solubility.

Subcellular localisation

Lysosome membrane

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Product protocols

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Target data

Endoglycosidase that cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans. Participates in extracellular matrix (ECM) degradation and remodeling. Selectively cleaves the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying either a 3-O-sulfo or a 6-O-sulfo group. Can also cleave the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying a 2-O-sulfo group, but not linkages between a glucuronic acid unit and a 2-O-sulfated iduronic acid moiety. It is essentially inactive at neutral pH but becomes active under acidic conditions such as during tumor invasion and in inflammatory processes. Facilitates cell migration associated with metastasis, wound healing and inflammation. Enhances shedding of syndecans, and increases endothelial invasion and angiogenesis in myelomas. Acts as a procoagulant by increasing the generation of activation factor X in the presence of tissue factor and activation factor VII. Increases cell adhesion to the extracellular matrix (ECM), independent of its enzymatic activity. Induces AKT1/PKB phosphorylation via lipid rafts increasing cell mobility and invasion. Heparin increases this AKT1/PKB activation. Regulates osteogenesis. Enhances angiogenesis through up-regulation of SRC-mediated activation of VEGF. Implicated in hair follicle inner root sheath differentiation and hair homeostasis.
See full target information HPSE
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Complementary Products

Primary Antibodies

AB288438

Anti-Heparanase 1 antibody [EPR25303-58]

20ul selling size|RabMAb|Recombinant|KO Validated|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heparanase 1 antibody [EPR25303-58] (AB288438)

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Assay Kits

AB256401

Human Heparanase ELISA Kit (HPSE)

Recombinant|SimpleStep

Sandwich ELISA - Human Heparanase ELISA Kit (HPSE) (AB256401)

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Cell Lines & Lysates

AB258456

Human HPSE (Heparanase 1) knockout HeLa cell lysate

Western blot - Human HPSE (Heparanase 1) knockout HeLa cell lysate (AB258456)

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Proteins & Peptides

AB276778

Recombinant Mouse VEGFD protein (His tag)

SDS-PAGE - Recombinant Mouse VEGFD protein (His tag) (AB276778)

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