Recombinant Human HEXB protein is a Human Full Length protein, expressed in Baculovirus infected Sf9, with >82% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >82% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- DDDDK tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M E L C G L G L P R P P M L L A L L L A T L L A A M L A L L T Q V A L V V Q V A E A A R A P S V S A K P G P A L W P L P L S V K M T P N L L H L A P E N F Y I S H S P N S T A G P S C T L L E E A F R R Y H G Y I F G F Y K W H H E P A E F Q A K T Q V Q Q L L V S I T L Q S E C D A F P N I S S D E S Y T L L V K E P V A V L K A N R V W G A L R G L E T F S Q L V Y Q D S Y G T F T I N E S T I I D S P R F S H R G I L I D T S R H Y L P V K I I L K T L D A M A F N K F N V L H W H I V D D Q S F P Y Q S I T F P E L S N K G S Y S L S H V Y T P N D V R M V I E Y A R L R G I R V L P E F D T P G H T L S W G K G Q K D L L T P C Y S R Q N K L D S F G P I N P T L N T T Y S F L T T F F K E I S E V F P D Q F I H L G G D E V E F K C W E S N P K I Q D F M R Q K G F G T D F K K L E S F Y I Q K V L D I I A T I N K G S I V W Q E V F D D K A K L A P G T I V E V W K D S A Y P E E L S R V T A S G F P V I L S A P W Y L D L I S Y G Q D W R K Y Y K V E P L D F G G T Q K Q K Q L F I G G E A C L W G E Y V D A T N L T P R L W P R A S A V G E R L W S S K D V R D M D D A Y D R L T R H R C R M V E R G I A A Q P L Y A G Y C N H E N M D Y K D D D D K H H H H H H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Hydrolyzes the non-reducing end N-acetyl-D-hexosamine and/or sulfated N-acetyl-D-hexosamine of glycoconjugates, such as the oligosaccharide moieties from proteins and neutral glycolipids, or from certain mucopolysaccharides (PubMed:11707436, PubMed:8123671, PubMed:8672428, PubMed:9694901). The isozyme B does not hydrolyze each of these substrates, however hydrolyzes efficiently neutral oligosaccharide (PubMed:11707436). Only the isozyme A is responsible for the degradation of GM2 gangliosides in the presence of GM2A (PubMed:8123671, PubMed:8672428, PubMed:9694901). During fertilization is responsible, at least in part, for the zona block to polyspermy. Present in the cortical granules of non-activated oocytes, is exocytosed during the cortical reaction in response to oocyte activation and inactivates the sperm galactosyltransferase-binding site, accounting for the block in sperm binding to the zona pellucida (By similarity).
Alternative names
HCC7, HEXB, Beta-hexosaminidase subunit beta, Beta-N-acetylhexosaminidase subunit beta, Cervical cancer proto-oncogene 7 protein, N-acetyl-beta-glucosaminidase subunit beta, Hexosaminidase subunit B, HCC-7
Recommended products
Recombinant Human HEXB protein is a Human Full Length protein, expressed in Baculovirus infected Sf9, with >82% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >82% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- DDDDK tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P07686-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Preservative: 1.02% Imidazole
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.04% Tween, 0.02% Potassium chloride
Sequence info
Amino acid sequence
- M E L C G L G L P R P P M L L A L L L A T L L A A M L A L L T Q V A L V V Q V A E A A R A P S V S A K P G P A L W P L P L S V K M T P N L L H L A P E N F Y I S H S P N S T A G P S C T L L E E A F R R Y H G Y I F G F Y K W H H E P A E F Q A K T Q V Q Q L L V S I T L Q S E C D A F P N I S S D E S Y T L L V K E P V A V L K A N R V W G A L R G L E T F S Q L V Y Q D S Y G T F T I N E S T I I D S P R F S H R G I L I D T S R H Y L P V K I I L K T L D A M A F N K F N V L H W H I V D D Q S F P Y Q S I T F P E L S N K G S Y S L S H V Y T P N D V R M V I E Y A R L R G I R V L P E F D T P G H T L S W G K G Q K D L L T P C Y S R Q N K L D S F G P I N P T L N T T Y S F L T T F F K E I S E V F P D Q F I H L G G D E V E F K C W E S N P K I Q D F M R Q K G F G T D F K K L E S F Y I Q K V L D I I A T I N K G S I V W Q E V F D D K A K L A P G T I V E V W K D S A Y P E E L S R V T A S G F P V I L S A P W Y L D L I S Y G Q D W R K Y Y K V E P L D F G G T Q K Q K Q L F I G G E A C L W G E Y V D A T N L T P R L W P R A S A V G E R L W S S K D V R D M D D A Y D R L T R H R C R M V E R G I A A Q P L Y A G Y C N H E N M D Y K D D D D K H H H H H H
- Accession
- P07686
- Protein length
- Full Length
- Predicted molecular weight
- 64.9 kDa
- Nature
- Recombinant
- Tags
- DDDDK tag C-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Hydrolyzes the non-reducing end N-acetyl-D-hexosamine and/or sulfated N-acetyl-D-hexosamine of glycoconjugates, such as the oligosaccharide moieties from proteins and neutral glycolipids, or from certain mucopolysaccharides (PubMed:11707436, PubMed:8123671, PubMed:8672428, PubMed:9694901). The isozyme B does not hydrolyze each of these substrates, however hydrolyzes efficiently neutral oligosaccharide (PubMed:11707436). Only the isozyme A is responsible for the degradation of GM2 gangliosides in the presence of GM2A (PubMed:8123671, PubMed:8672428, PubMed:9694901). During fertilization is responsible, at least in part, for the zona block to polyspermy. Present in the cortical granules of non-activated oocytes, is exocytosed during the cortical reaction in response to oocyte activation and inactivates the sperm galactosyltransferase-binding site, accounting for the block in sperm binding to the zona pellucida (By similarity).
- Sequence similarities
Belongs to the glycosyl hydrolase 20 family.
- Post-translational modifications
N-linked glycans at Asn-142 and Asn-190 consist of Man(3)-GlcNAc(2) and Man(5 to 7)-GlcNAc(2), respectively.
- Subcellular localisation
- Lysosome
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The target known as HEXB also called Hexosaminidase subunit beta consists of protein subunit that helps in the formation of the enzyme beta-hexosaminidase. This enzyme is active in lysosomes and it weighs about 63 kDa. Beta-hexosaminidase functions to break down GM2 gangliosides which are molecules in cell membranes. HEXB expresses mainly in the brain and liver tissues signaling its role in neurological and hepatic processes.
- Biological function summary
The beta-hexosaminidase enzyme formed by HEXB participates in the lysosomal degradation pathway. This enzyme requires formation of a complex with its counterpart HEXA to become biologically active. Together they catalyze the hydrolysis of GM2 gangliosides into GM3 gangliosides which is an essential step for cell membrane metabolism. This process is important for maintaining cellular homeostasis and preventing accumulation of toxic substances within cells.
- Pathways
The HEXB encoded enzyme participates in sphingolipid metabolism an integral component of lipid metabolic pathways. Sphingolipids are part of many cellular functions including signal transmission and cell recognition. HEXB through the lysosomal degradation of gangliosides works closely with HEXA and NEU1 which further processes GM3 gangliosides into simpler molecules. Such interactions underline HEXB’s significant role in maintaining the balance of lipid metabolism within cells.
- Associated diseases and disorders
Mutations or deficiencies in HEXB lead to serious genetic conditions such as Sandhoff disease. This disorder results from the improper breakdown of gangliosides due to the lack of functional HEXB leading to their accumulation which severely damages nerve cells and results in neurological impairments. The partnership between HEXB and HEXA emphasizes the importance of both subunits as malfunction in either can contribute to disease progression by disrupting normal ganglioside metabolism.
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1 product image
SDS-PAGE - Recombinant Human HEXB protein (ab198424)
4-20% SDS-PAGE Coomassie staining.
Lane 1: 2.8 μg ab198424
Lane 2: Protein Marker
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