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Recombinant human Hexokinase 1 protein is a Human Full Length protein, in the 1 to 917 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, WB, FuncS.

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Images

SDS-PAGE - Recombinant human Hexokinase 1 protein (AB85918), expandable thumbnail
  • Western blot - Recombinant human Hexokinase 1 protein (AB85918), expandable thumbnail
  • Western blot - Recombinant human Hexokinase 1 protein (AB85918), expandable thumbnail

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, WB, FuncS

Biologically active

Yes

Amino acid sequence

M G S S H H H H H H S S G L V P R G S H M I A A Q L L A Y Y F T E L K D D Q V K K I D K Y L Y A M R L S D E T L I D I M T R F R K E M K N G L S R D F N P T A T V K M L P T F V R S I P D G S E K G D F I A L D L G G S S F R I L R V Q V N H E K N Q N V H M E S E V Y D T P E N I V H G S G S Q L F D H V A E C L G D F M E K R K I K D K K L P V G F T F S F P C Q Q S K I D E A I L I T W T K R F K A S G V E G A D V V K L L N K A I K K R G D Y D A N I V A V V N D T V G T M M T C G Y D D Q H C E V G L I I G T G T N A C Y M E E L R H I D L V E G D E G R M C I N T E W G A F G D D G S L E D I R T E F D R E I D R G S L N P G K Q L F E K M V S G M Y L G E L V R L I L V K M A K E G L L F E G R I T P E L L T R G K F N T S D V S A I E K N K E G L H N A K E I L T R L G V E P S D D D C V S V Q H V C T I V S F R S A N L V A A T L G A I L N R L R D N K G T P R L R T T V G V D G S L Y K T H P Q Y S R R F H K T L R R L V P D S D V R F L L S E S G S G K G A A M V T A V A Y R L A E Q H R Q I E E T L A H F H L T K D M L L E V K K R M R A E M E L G L R K Q T H N N A V V K M L P S F V R R T P D G T E N G D F L A L D L G G T N F R V L L V K I R S G K K R T V E M H N K I Y A I P I E I M Q G T G E E L F D H I V S C I S D F L D Y M G I K G P R M P L G F T F S F P C Q Q T S L D A G I L I T W T K G F K A T D C V G H D V V T L L R D A I K R R E E F D L D V V A V V N D T V G T M M T C A Y E E P T C E V G L I V G T G S N A C Y M E E M K N V E M V E G D Q G Q M C I N M E W G A F G D N G C L D D I R T H Y D R L V D E Y S L N A G K Q R Y E K M I S G M Y L G E I V R N I L I D F T K K G F L F R G Q I S E T L K T R G I F E T K F L S Q I E S D R L A L L Q V R A I L Q Q L G L N S T C D D S I L V K T V C G V V S R R A A Q L C G A G M A A V V D K I R E N R G L D R L N V T V G V D G T L Y K L H P H F S R I M H Q T V K E L S P K C N V S F L L S E D G S G K G A A L I T A V G V R L R T E A S S

Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Application

WB

Reactivity

Reacts

Dilution info

-

Notes

ab85918 can be used as a WB positive control in conjunction with Anti-Hexokinase 1 antibody - Mitochondrial Outer Membrane Marker ab65069.

Application

FuncS

Reactivity

Reacts

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Catalyzes the phosphorylation of various hexoses, such as D-glucose, D-glucosamine, D-fructose, D-mannose and 2-deoxy-D-glucose, to hexose 6-phosphate (D-glucose 6-phosphate, D-glucosamine 6-phosphate, D-fructose 6-phosphate, D-mannose 6-phosphate and 2-deoxy-D-glucose 6-phosphate, respectively) (PubMed:1637300, PubMed:25316723, PubMed:27374331). Does not phosphorylate N-acetyl-D-glucosamine (PubMed:27374331). Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate (By similarity). Involved in innate immunity and inflammation by acting as a pattern recognition receptor for bacterial peptidoglycan (PubMed:27374331). When released in the cytosol, N-acetyl-D-glucosamine component of bacterial peptidoglycan inhibits the hexokinase activity of HK1 and causes its dissociation from mitochondrial outer membrane, thereby activating the NLRP3 inflammasome (PubMed:27374331).

Alternative names

Recommended products

Recombinant human Hexokinase 1 protein is a Human Full Length protein, in the 1 to 917 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, WB, FuncS.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Applications

SDS-PAGE, WB, FuncS

Biological activity

Specific activity is >2 units/ml obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP. In the coupled mode, one unit will produce 1.0 umole of NADPH per minute as glucose is phosphorylated by ATP at pH 7.4 at 25C. Specific activity is >2 units/ml obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP. In the coupled mode, one unit will produce 1.0 umole of NADPH per minute as glucose is phosphorylated by ATP at pH 7.4 at 25C.

Accession
P19367-1
Animal free

No

Species

Human

Concentration
Loading...
Storage buffer

pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl

Sequence info

Amino acid sequence

M G S S H H H H H H S S G L V P R G S H M I A A Q L L A Y Y F T E L K D D Q V K K I D K Y L Y A M R L S D E T L I D I M T R F R K E M K N G L S R D F N P T A T V K M L P T F V R S I P D G S E K G D F I A L D L G G S S F R I L R V Q V N H E K N Q N V H M E S E V Y D T P E N I V H G S G S Q L F D H V A E C L G D F M E K R K I K D K K L P V G F T F S F P C Q Q S K I D E A I L I T W T K R F K A S G V E G A D V V K L L N K A I K K R G D Y D A N I V A V V N D T V G T M M T C G Y D D Q H C E V G L I I G T G T N A C Y M E E L R H I D L V E G D E G R M C I N T E W G A F G D D G S L E D I R T E F D R E I D R G S L N P G K Q L F E K M V S G M Y L G E L V R L I L V K M A K E G L L F E G R I T P E L L T R G K F N T S D V S A I E K N K E G L H N A K E I L T R L G V E P S D D D C V S V Q H V C T I V S F R S A N L V A A T L G A I L N R L R D N K G T P R L R T T V G V D G S L Y K T H P Q Y S R R F H K T L R R L V P D S D V R F L L S E S G S G K G A A M V T A V A Y R L A E Q H R Q I E E T L A H F H L T K D M L L E V K K R M R A E M E L G L R K Q T H N N A V V K M L P S F V R R T P D G T E N G D F L A L D L G G T N F R V L L V K I R S G K K R T V E M H N K I Y A I P I E I M Q G T G E E L F D H I V S C I S D F L D Y M G I K G P R M P L G F T F S F P C Q Q T S L D A G I L I T W T K G F K A T D C V G H D V V T L L R D A I K R R E E F D L D V V A V V N D T V G T M M T C A Y E E P T C E V G L I V G T G S N A C Y M E E M K N V E M V E G D Q G Q M C I N M E W G A F G D N G C L D D I R T H Y D R L V D E Y S L N A G K Q R Y E K M I S G M Y L G E I V R N I L I D F T K K G F L F R G Q I S E T L K T R G I F E T K F L S Q I E S D R L A L L Q V R A I L Q Q L G L N S T C D D S I L V K T V C G V V S R R A A Q L C G A G M A A V V D K I R E N R G L D R L N V T V G V D G T L Y K L H P H F S R I M H Q T V K E L S P K C N V S F L L S E D G S G K G A A L I T A V G V R L R T E A S S

Accession

P19367

Protein length

Full Length

Amino acids

1 to 917

Nature

Recombinant

Tags

His tag N-Terminus

Specifications

Form

Liquid

Additional notes

ab85918 is purified using conventional chromatography techniques.

General info

Function

Catalyzes the phosphorylation of various hexoses, such as D-glucose, D-glucosamine, D-fructose, D-mannose and 2-deoxy-D-glucose, to hexose 6-phosphate (D-glucose 6-phosphate, D-glucosamine 6-phosphate, D-fructose 6-phosphate, D-mannose 6-phosphate and 2-deoxy-D-glucose 6-phosphate, respectively) (PubMed:1637300, PubMed:25316723, PubMed:27374331). Does not phosphorylate N-acetyl-D-glucosamine (PubMed:27374331). Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate (By similarity). Involved in innate immunity and inflammation by acting as a pattern recognition receptor for bacterial peptidoglycan (PubMed:27374331). When released in the cytosol, N-acetyl-D-glucosamine component of bacterial peptidoglycan inhibits the hexokinase activity of HK1 and causes its dissociation from mitochondrial outer membrane, thereby activating the NLRP3 inflammasome (PubMed:27374331).

Sequence similarities

Belongs to the hexokinase family.

Subcellular localisation

Mitochondrion outer membrane

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Product promise

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Full details and terms and conditions can be found here:
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3 product images

  • SDS-PAGE - Recombinant human Hexokinase 1 protein (ab85918), expandable thumbnail

    SDS-PAGE - Recombinant human Hexokinase 1 protein (ab85918)

    12% SDS-PAGE showing ab85918 at approximately 104.6kDa.

  • Western blot - Recombinant human Hexokinase 1 protein (ab85918), expandable thumbnail

    Western blot - Recombinant human Hexokinase 1 protein (ab85918)

    All lanes: Western blot - Anti-Hexokinase 1 antibody - Mitochondrial Outer Membrane Marker (Anti-Hexokinase 1 antibody - Mitochondrial Outer Membrane Marker ab65069) at 1/500 dilution

    All lanes: Western blot - Recombinant human Hexokinase 1 protein (ab85918) at 0.1 µg

    Secondary

    All lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 102 kDa

    Exposure time: 4min

  • Western blot - Recombinant human Hexokinase 1 protein (ab85918), expandable thumbnail

    Western blot - Recombinant human Hexokinase 1 protein (ab85918)

    All lanes: Western blot - Anti-Hexokinase 1 antibody (Anti-Hexokinase 1 antibody ab150013) at 1/1 dilution

    All lanes: Western blot - Recombinant human Hexokinase 1 protein (ab85918) at 0.1 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 102 kDa

    Observed band size: 102 kDa

    Exposure time: 1min

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Product protocols

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