Recombinant human Hexokinase 1 protein is a Human Full Length protein, in the 1 to 917 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, WB, FuncS.
>90% SDS-PAGE
Escherichia coli
His tag N-Terminus
SDS-PAGE, WB, FuncS
Yes
M G S S H H H H H H S S G L V P R G S H M I A A Q L L A Y Y F T E L K D D Q V K K I D K Y L Y A M R L S D E T L I D I M T R F R K E M K N G L S R D F N P T A T V K M L P T F V R S I P D G S E K G D F I A L D L G G S S F R I L R V Q V N H E K N Q N V H M E S E V Y D T P E N I V H G S G S Q L F D H V A E C L G D F M E K R K I K D K K L P V G F T F S F P C Q Q S K I D E A I L I T W T K R F K A S G V E G A D V V K L L N K A I K K R G D Y D A N I V A V V N D T V G T M M T C G Y D D Q H C E V G L I I G T G T N A C Y M E E L R H I D L V E G D E G R M C I N T E W G A F G D D G S L E D I R T E F D R E I D R G S L N P G K Q L F E K M V S G M Y L G E L V R L I L V K M A K E G L L F E G R I T P E L L T R G K F N T S D V S A I E K N K E G L H N A K E I L T R L G V E P S D D D C V S V Q H V C T I V S F R S A N L V A A T L G A I L N R L R D N K G T P R L R T T V G V D G S L Y K T H P Q Y S R R F H K T L R R L V P D S D V R F L L S E S G S G K G A A M V T A V A Y R L A E Q H R Q I E E T L A H F H L T K D M L L E V K K R M R A E M E L G L R K Q T H N N A V V K M L P S F V R R T P D G T E N G D F L A L D L G G T N F R V L L V K I R S G K K R T V E M H N K I Y A I P I E I M Q G T G E E L F D H I V S C I S D F L D Y M G I K G P R M P L G F T F S F P C Q Q T S L D A G I L I T W T K G F K A T D C V G H D V V T L L R D A I K R R E E F D L D V V A V V N D T V G T M M T C A Y E E P T C E V G L I V G T G S N A C Y M E E M K N V E M V E G D Q G Q M C I N M E W G A F G D N G C L D D I R T H Y D R L V D E Y S L N A G K Q R Y E K M I S G M Y L G E I V R N I L I D F T K K G F L F R G Q I S E T L K T R G I F E T K F L S Q I E S D R L A L L Q V R A I L Q Q L G L N S T C D D S I L V K T V C G V V S R R A A Q L C G A G M A A V V D K I R E N R G L D R L N V T V G V D G T L Y K L H P H F S R I M H Q T V K E L S P K C N V S F L L S E D G S G K G A A L I T A V G V R L R T E A S S
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes ab85918 can be used as a WB positive control in conjunction with Anti-Hexokinase 1 antibody - Mitochondrial Outer Membrane Marker ab65069. |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Select an associated product type
Catalyzes the phosphorylation of various hexoses, such as D-glucose, D-glucosamine, D-fructose, D-mannose and 2-deoxy-D-glucose, to hexose 6-phosphate (D-glucose 6-phosphate, D-glucosamine 6-phosphate, D-fructose 6-phosphate, D-mannose 6-phosphate and 2-deoxy-D-glucose 6-phosphate, respectively) (PubMed:1637300, PubMed:25316723, PubMed:27374331). Does not phosphorylate N-acetyl-D-glucosamine (PubMed:27374331). Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate (By similarity). Involved in innate immunity and inflammation by acting as a pattern recognition receptor for bacterial peptidoglycan (PubMed:27374331). When released in the cytosol, N-acetyl-D-glucosamine component of bacterial peptidoglycan inhibits the hexokinase activity of HK1 and causes its dissociation from mitochondrial outer membrane, thereby activating the NLRP3 inflammasome (PubMed:27374331).
Hexokinase-1, Brain form hexokinase, Hexokinase type I, Hexokinase-A, HK I, HK1
Recombinant human Hexokinase 1 protein is a Human Full Length protein, in the 1 to 917 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, WB, FuncS.
>90% SDS-PAGE
Escherichia coli
His tag N-Terminus
SDS-PAGE, WB, FuncS
Yes
Specific activity is >2 units/ml obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP. In the coupled mode, one unit will produce 1.0 umole of NADPH per minute as glucose is phosphorylated by ATP at pH 7.4 at 25C. Specific activity is >2 units/ml obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP. In the coupled mode, one unit will produce 1.0 umole of NADPH per minute as glucose is phosphorylated by ATP at pH 7.4 at 25C.
No
Human
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl
M G S S H H H H H H S S G L V P R G S H M I A A Q L L A Y Y F T E L K D D Q V K K I D K Y L Y A M R L S D E T L I D I M T R F R K E M K N G L S R D F N P T A T V K M L P T F V R S I P D G S E K G D F I A L D L G G S S F R I L R V Q V N H E K N Q N V H M E S E V Y D T P E N I V H G S G S Q L F D H V A E C L G D F M E K R K I K D K K L P V G F T F S F P C Q Q S K I D E A I L I T W T K R F K A S G V E G A D V V K L L N K A I K K R G D Y D A N I V A V V N D T V G T M M T C G Y D D Q H C E V G L I I G T G T N A C Y M E E L R H I D L V E G D E G R M C I N T E W G A F G D D G S L E D I R T E F D R E I D R G S L N P G K Q L F E K M V S G M Y L G E L V R L I L V K M A K E G L L F E G R I T P E L L T R G K F N T S D V S A I E K N K E G L H N A K E I L T R L G V E P S D D D C V S V Q H V C T I V S F R S A N L V A A T L G A I L N R L R D N K G T P R L R T T V G V D G S L Y K T H P Q Y S R R F H K T L R R L V P D S D V R F L L S E S G S G K G A A M V T A V A Y R L A E Q H R Q I E E T L A H F H L T K D M L L E V K K R M R A E M E L G L R K Q T H N N A V V K M L P S F V R R T P D G T E N G D F L A L D L G G T N F R V L L V K I R S G K K R T V E M H N K I Y A I P I E I M Q G T G E E L F D H I V S C I S D F L D Y M G I K G P R M P L G F T F S F P C Q Q T S L D A G I L I T W T K G F K A T D C V G H D V V T L L R D A I K R R E E F D L D V V A V V N D T V G T M M T C A Y E E P T C E V G L I V G T G S N A C Y M E E M K N V E M V E G D Q G Q M C I N M E W G A F G D N G C L D D I R T H Y D R L V D E Y S L N A G K Q R Y E K M I S G M Y L G E I V R N I L I D F T K K G F L F R G Q I S E T L K T R G I F E T K F L S Q I E S D R L A L L Q V R A I L Q Q L G L N S T C D D S I L V K T V C G V V S R R A A Q L C G A G M A A V V D K I R E N R G L D R L N V T V G V D G T L Y K L H P H F S R I M H Q T V K E L S P K C N V S F L L S E D G S G K G A A L I T A V G V R L R T E A S S
Full Length
1 to 917
Recombinant
His tag N-Terminus
Liquid
ab85918 is purified using conventional chromatography techniques.
Catalyzes the phosphorylation of various hexoses, such as D-glucose, D-glucosamine, D-fructose, D-mannose and 2-deoxy-D-glucose, to hexose 6-phosphate (D-glucose 6-phosphate, D-glucosamine 6-phosphate, D-fructose 6-phosphate, D-mannose 6-phosphate and 2-deoxy-D-glucose 6-phosphate, respectively) (PubMed:1637300, PubMed:25316723, PubMed:27374331). Does not phosphorylate N-acetyl-D-glucosamine (PubMed:27374331). Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate (By similarity). Involved in innate immunity and inflammation by acting as a pattern recognition receptor for bacterial peptidoglycan (PubMed:27374331). When released in the cytosol, N-acetyl-D-glucosamine component of bacterial peptidoglycan inhibits the hexokinase activity of HK1 and causes its dissociation from mitochondrial outer membrane, thereby activating the NLRP3 inflammasome (PubMed:27374331).
Belongs to the hexokinase family.
Mitochondrion outer membrane
Blue Ice
-20°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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Full details and terms and conditions can be found here:
Terms & Conditions.
12% SDS-PAGE showing ab85918 at approximately 104.6kDa.
All lanes: Western blot - Anti-Hexokinase 1 antibody - Mitochondrial Outer Membrane Marker (Anti-Hexokinase 1 antibody - Mitochondrial Outer Membrane Marker ab65069) at 1/500 dilution
All lanes: Western blot - Recombinant human Hexokinase 1 protein (ab85918) at 0.1 µg
All lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 102 kDa
Exposure time: 4min
All lanes: Western blot - Anti-Hexokinase 1 antibody (Anti-Hexokinase 1 antibody ab150013) at 1/1 dilution
All lanes: Western blot - Recombinant human Hexokinase 1 protein (ab85918) at 0.1 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 102 kDa
Observed band size: 102 kDa
Exposure time: 1min
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