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AB85918

Recombinant human Hexokinase 1 protein

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Recombinant human Hexokinase 1 protein is a Human Full Length protein, in the 1 to 917 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, WB, FuncS.

View Alternative Names

Hexokinase-1, Brain form hexokinase, Hexokinase type I, Hexokinase-A, HK I, HK1

3 Images
Western blot - Recombinant human Hexokinase 1 protein (AB85918)
  • WB

Unknown

Western blot - Recombinant human Hexokinase 1 protein (AB85918)

All lanes:

Western blot - Anti-Hexokinase 1 antibody - Mitochondrial Outer Membrane Marker (<a href='/en-us/products/primary-antibodies/hexokinase-1-antibody-mitochondrial-outer-membrane-marker-ab65069'>ab65069</a>) at 1/500 dilution

All lanes:

Western blot - Recombinant human Hexokinase 1 protein (ab85918) at 0.1 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/5000 dilution

Predicted band size: 102 kDa

true

Exposure time: 4min

Western blot - Recombinant human Hexokinase 1 protein (AB85918)
  • WB

Unknown

Western blot - Recombinant human Hexokinase 1 protein (AB85918)

All lanes:

Western blot - Anti-Hexokinase 1 antibody (<a href='/en-us/products/primary-antibodies/hexokinase-1-antibody-ab150013'>ab150013</a>) at 1/1 dilution

All lanes:

Western blot - Recombinant human Hexokinase 1 protein (ab85918) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 102 kDa

Observed band size: 102 kDa

true

Exposure time: 1min

SDS-PAGE - Recombinant human Hexokinase 1 protein (AB85918)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human Hexokinase 1 protein (AB85918)

12% SDS-PAGE showing ab85918 at approximately 104.6kDa.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

FuncS, SDS-PAGE, WB

applications

Biologically active

Yes

Biological activity

Specific activity is >2 units/ml obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP. In the coupled mode, one unit will produce 1.0 umole of NADPH per minute as glucose is phosphorylated by ATP at pH 7.4 at 25C. Specific activity is >2 units/ml obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP. In the coupled mode, one unit will produce 1.0 umole of NADPH per minute as glucose is phosphorylated by ATP at pH 7.4 at 25C.

Accession

P19367

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>ab85918 can be used as a WB positive control in conjunction with <a href='/en-us/products/primary-antibodies/hexokinase-1-antibody-mitochondrial-outer-membrane-marker-ab65069'>ab65069</a>.</p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMIAAQLLAYYFTELKDDQVKKIDKYLYAMRLSDETLIDIMTRFRKEMKNGLSRDFNPTATVKMLPTFVRSIPDGSEKGDFIALDLGGSSFRILRVQVNHEKNQNVHMESEVYDTPENIVHGSGSQLFDHVAECLGDFMEKRKIKDKKLPVGFTFSFPCQQSKIDEAILITWTKRFKASGVEGADVVKLLNKAIKKRGDYDANIVAVVNDTVGTMMTCGYDDQHCEVGLIIGTGTNACYMEELRHIDLVEGDEGRMCINTEWGAFGDDGSLEDIRTEFDREIDRGSLNPGKQLFEKMVSGMYLGELVRLILVKMAKEGLLFEGRITPELLTRGKFNTSDVSAIEKNKEGLHNAKEILTRLGVEPSDDDCVSVQHVCTIVSFRSANLVAATLGAILNRLRDNKGTPRLRTTVGVDGSLYKTHPQYSRRFHKTLRRLVPDSDVRFLLSESGSGKGAAMVTAVAYRLAEQHRQIEETLAHFHLTKDMLLEVKKRMRAEMELGLRKQTHNNAVVKMLPSFVRRTPDGTENGDFLALDLGGTNFRVLLVKIRSGKKRTVEMHNKIYAIPIEIMQGTGEELFDHIVSCISDFLDYMGIKGPRMPLGFTFSFPCQQTSLDAGILITWTKGFKATDCVGHDVVTLLRDAIKRREEFDLDVVAVVNDTVGTMMTCAYEEPTCEVGLIVGTGSNACYMEEMKNVEMVEGDQGQMCINMEWGAFGDNGCLDDIRTHYDRLVDEYSLNAGKQRYEKMISGMYLGEIVRNILIDFTKKGFLFRGQISETLKTRGIFETKFLSQIESDRLALLQVRAILQQLGLNSTCDDSILVKTVCGVVSRRAAQLCGAGMAAVVDKIRENRGLDRLNVTVGVDGTLYKLHPHFSRIMHQTVKELSPKCNVSFLLSEDGSGKGAALITAVGVRLRTEASS","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":917,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P19367","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Specifications

Form

Liquid

Additional notes

ab85918 is purified using conventional chromatography techniques.

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General info

Function

Catalyzes the phosphorylation of various hexoses, such as D-glucose, D-glucosamine, D-fructose, D-mannose and 2-deoxy-D-glucose, to hexose 6-phosphate (D-glucose 6-phosphate, D-glucosamine 6-phosphate, D-fructose 6-phosphate, D-mannose 6-phosphate and 2-deoxy-D-glucose 6-phosphate, respectively) (PubMed : 1637300, PubMed : 25316723, PubMed : 27374331). Does not phosphorylate N-acetyl-D-glucosamine (PubMed : 27374331). Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate (By similarity). Involved in innate immunity and inflammation by acting as a pattern recognition receptor for bacterial peptidoglycan (PubMed : 27374331). When released in the cytosol, N-acetyl-D-glucosamine component of bacterial peptidoglycan inhibits the hexokinase activity of HK1 and causes its dissociation from mitochondrial outer membrane, thereby activating the NLRP3 inflammasome (PubMed : 27374331).

Sequence similarities

Belongs to the hexokinase family.

Subcellular localisation

Mitochondrion outer membrane

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Product protocols

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Target data

Catalyzes the phosphorylation of various hexoses, such as D-glucose, D-glucosamine, D-fructose, D-mannose and 2-deoxy-D-glucose, to hexose 6-phosphate (D-glucose 6-phosphate, D-glucosamine 6-phosphate, D-fructose 6-phosphate, D-mannose 6-phosphate and 2-deoxy-D-glucose 6-phosphate, respectively) (PubMed : 1637300, PubMed : 25316723, PubMed : 27374331). Does not phosphorylate N-acetyl-D-glucosamine (PubMed : 27374331). Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate (By similarity). Involved in innate immunity and inflammation by acting as a pattern recognition receptor for bacterial peptidoglycan (PubMed : 27374331). When released in the cytosol, N-acetyl-D-glucosamine component of bacterial peptidoglycan inhibits the hexokinase activity of HK1 and causes its dissociation from mitochondrial outer membrane, thereby activating the NLRP3 inflammasome (PubMed : 27374331).
See full target information HK1
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Complementary Products

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AB85920

Recombinant human Hexokinase II protein (His tag)

SDS-PAGE - Recombinant human Hexokinase II protein (His tag) (AB85920)

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Primary Antibodies

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Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker

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Immunocytochemistry/ Immunofluorescence - Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker (AB150423)

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Western blot - Recombinant human Lactate Dehydrogenase protein (Active) (AB93699)

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Primary Antibodies

AB209847

Anti-Hexokinase II antibody [EPR20839]

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Western blot - Anti-Hexokinase II antibody [EPR20839] (AB209847)

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