Recombinant human Hexokinase II protein (His tag)

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, FuncS

Biologically active

Yes

Biological activity

Specific activity is 3-4 units/ml obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP. In the coupled mode, one unit will produce 1.0 umole of NADPH per minute as glucose is phosphorylated by ATP at pH 7.4 at 30C. Specific activity is 3-4 units/ml obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP. In the coupled mode, one unit will produce 1.0 umole of NADPH per minute as glucose is phosphorylated by ATP at pH 7.4 at 30C.

Accession

P52789-1

Animal free

No

Species

Human

Concentration

Storage buffer

pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl

Sequence info

Amino acid sequence

M G S S H H H H H H S S G L V P R G S H M I A S H L L A Y F F T E L N H D Q V Q K V D Q Y L Y H M R L S D E T L L E I S K R F R K E M E K G L G A T T H P T A A V K M L P T F V R S T P D G T E H G E F L A L D L G G T N F R V L W V K V T D N G L Q K V E M E N Q I Y A I P E D I M R G S G T Q L F D H I A E C L A N F M D K L Q I K D K K L P L G F T F S F P C H Q T K L D E S F L V S W T K G F K S S G V E G R D V V A L I R K A I Q R R G D F D I D I V A V V N D T V G T M M T C G Y D D H N C E I G L I V G T G S N A C Y M E E M R H I D M V E G D E G R M C I N M E W G A F G D D G S L N D I R T E F D Q E I D M G S L N P G K Q L F E K M I S G M Y M G E L V R L I L V K M A K E E L L F G G K L S P E L L N T G R F E T K D I S D I E G E K D G I R K A R E V L M R L G L D P T Q E D C V A T H R I C Q I V S T R S A S L C A A T L A A V L Q R I K E N K G E E R L R S T I G V D G S V Y K K H P H F A K R L H K T V R R L V P G C D V R F L R S E D G S G K G A A M V T A V A Y R L A D Q H R A R Q K T L E H L Q L S H D Q L L E V K R R M K V E M E R G L S K E T H A S A P V K M L P T Y V C A T P D G T E K G D F L A L D L G G T N F R V L L V R V R N G K W G G V E M H N K I Y A I P Q E V M H G T G D E L F D H I V Q C I A D F L E Y M G M K G V S L P L G F T F S F P C Q Q N S L D E S I L L K W T K G F K A S G C E G E D V V T L L K E A I H R R E E F D L D V V A V V N D T V G T M M T C G F E D P H C E V G L I V G T G S N A C Y M E E M R N V E L V E G E E G R M C V N M E W G A F G D N G C L D D F R T E F D V A V D E L S L N P G K Q R F E K M I S G M Y L G E I V R N I L I D F T K R G L L F R G R I S E R L K T R G I F E T K F L S Q I E S D C L A L L Q V R A I L Q H L G L E S T C D D S I I V K E V C T V V A R R A A Q L C G A G M A A V V D R I R E N R G L D A L K V T V G V D G T L Y K L H P H F A K V M H E T V K D L A P K C D V S F L Q S E D G S G K G A A L I T A V A C R I R E A G Q R

Accession

P52789

Protein length

Full Length

Predicted molecular weight

102 kDa

Amino acids

1 to 917

Nature

Recombinant

Tags

His tag N-Terminus

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