Recombinant Human HFE protein (denatured) is a Human Fragment protein, in the 23 to 306 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M R L L R S H S L H Y L F M G A S E Q D L G L S L F E A L G Y V D D Q L F V F Y D H E S R R V E P R T P W V S S R I S S Q M W L Q L S Q S L K G W D H M F T V D F W T I M E N H N H S K E S H T L Q V I L G C E M Q E D N S T E G Y W K Y G Y D G Q D H L E F C P D T L D W R A A E P R A W P T K L E W E R H K I R A R Q N R A Y L E R D C P A Q L Q Q L L E L G R G V L D Q Q V P P L V K V T H H V T S S V T T L R C R A L N Y Y P Q N I T M K W L K D K Q P M D A K E F E P K D V L P N G D G T Y Q G W I T L A V P P G E E Q R Y T C Q V E H P G L D Q P L I V I W E P S P S G T L V
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Binds to transferrin receptor (TFR) and reduces its affinity for iron-loaded transferrin.
Alternative names
HLAH, HFE, Hereditary hemochromatosis protein, HLA-H
Recommended products
Recombinant Human HFE protein (denatured) is a Human Fragment protein, in the 23 to 306 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q30201-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris HCl
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M R L L R S H S L H Y L F M G A S E Q D L G L S L F E A L G Y V D D Q L F V F Y D H E S R R V E P R T P W V S S R I S S Q M W L Q L S Q S L K G W D H M F T V D F W T I M E N H N H S K E S H T L Q V I L G C E M Q E D N S T E G Y W K Y G Y D G Q D H L E F C P D T L D W R A A E P R A W P T K L E W E R H K I R A R Q N R A Y L E R D C P A Q L Q Q L L E L G R G V L D Q Q V P P L V K V T H H V T S S V T T L R C R A L N Y Y P Q N I T M K W L K D K Q P M D A K E F E P K D V L P N G D G T Y Q G W I T L A V P P G E E Q R Y T C Q V E H P G L D Q P L I V I W E P S P S G T L V
- Accession
- Q30201
- Protein length
- Fragment
- Predicted molecular weight
- 35.7 kDa
- Amino acids
- 23 to 306
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Binds to transferrin receptor (TFR) and reduces its affinity for iron-loaded transferrin.
- Sequence similarities
Belongs to the MHC class I family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The HFE protein also known as the hemochromatosis protein or HFE 5000 is a critical component in the regulation of iron absorption in the body. Structurally this protein has a known mass of about 50 kDa and is expressed primarily in the liver pancreas and intestines. The HFE belongs to the class I major histocompatibility complex (MHC) proteins. Its main function is to interact with transferrin receptors regulating the amount of iron that's absorbed from the diet into the bloodstream.
- Biological function summary
This protein plays a significant role in maintaining iron homeostasis within the body. HFE does not function alone; it forms a complex with transferrin receptors to modulate iron uptake. Its interaction with the transferrin-bound iron helps prevent excess iron build-up by reducing the binding affinity of transferrin for its receptor. Through these interactions HFE balances iron storage and transport ensuring proper iron levels in vital organs.
- Pathways
HFE is an integral part of iron metabolism and homeostasis pathways. It closely interacts with proteins like TFR1 (Transferrin Receptor 1) in these pathways. The hepcidin pathway also involves HFE where it indirectly influences the synthesis of hepcidin by the liver. Hepcidin plays an important role in preventing iron overload by binding and degrading the iron export protein ferroportin hence regulating the release of iron from cells adding a layer of control over iron homeostasis.
- Associated diseases and disorders
HFE mutations can lead to hereditary hemochromatosis a condition characterized by excessive iron accumulation in tissues which leads to organ damage. A common mutation is the C282Y mutation which disrupts the protein's normal function. This disorder links to other proteins related to iron metabolism such as hepcidin. Proper functioning of HFE is essential to avoid diseases like hemochromatosis as its impairment results in misregulated iron levels leading to adverse health effects.
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1 product image
SDS-PAGE - Recombinant Human HFE protein (denatured) (ab139241)
15% SDS-PAGE analysis of 3 µg ab139241.
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