JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB139241

Recombinant Human HFE protein (denatured) (His tag N-Terminus)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human HFE protein (denatured) (His tag N-Terminus) is a Human Fragment protein, in the 23 to 306 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

View Alternative Names

HLAH, HFE, Hereditary hemochromatosis protein, HLA-H

1 Images
SDS-PAGE - Recombinant Human HFE protein (denatured) (His tag N-Terminus) (AB139241)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human HFE protein (denatured) (His tag N-Terminus) (AB139241)

15% SDS-PAGE analysis of 3 µg ab139241.

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q30201

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris HCl

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMRLLRSHSLHYLFMGASEQDLGLSLFEALGYVDDQLFVFYDHESRRVEPRTPWVSSRISSQMWLQLSQSLKGWDHMFTVDFWTIMENHNHSKESHTLQVILGCEMQEDNSTEGYWKYGYDGQDHLEFCPDTLDWRAAEPRAWPTKLEWERHKIRARQNRAYLERDCPAQLQQLLELGRGVLDQQVPPLVKVTHHVTSSVTTLRCRALNYYPQNITMKWLKDKQPMDAKEFEPKDVLPNGDGTYQGWITLAVPPGEEQRYTCQVEHPGLDQPLIVIWEPSPSGTLV","proteinLength":"Fragment","predictedMolecularWeight":"35.7 kDa","actualMolecularWeight":null,"aminoAcidEnd":306,"aminoAcidStart":23,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q30201","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The HFE protein also known as the hemochromatosis protein or HFE 5000 is a critical component in the regulation of iron absorption in the body. Structurally this protein has a known mass of about 50 kDa and is expressed primarily in the liver pancreas and intestines. The HFE belongs to the class I major histocompatibility complex (MHC) proteins. Its main function is to interact with transferrin receptors regulating the amount of iron that's absorbed from the diet into the bloodstream.
Biological function summary

This protein plays a significant role in maintaining iron homeostasis within the body. HFE does not function alone; it forms a complex with transferrin receptors to modulate iron uptake. Its interaction with the transferrin-bound iron helps prevent excess iron build-up by reducing the binding affinity of transferrin for its receptor. Through these interactions HFE balances iron storage and transport ensuring proper iron levels in vital organs.

Pathways

HFE is an integral part of iron metabolism and homeostasis pathways. It closely interacts with proteins like TFR1 (Transferrin Receptor 1) in these pathways. The hepcidin pathway also involves HFE where it indirectly influences the synthesis of hepcidin by the liver. Hepcidin plays an important role in preventing iron overload by binding and degrading the iron export protein ferroportin hence regulating the release of iron from cells adding a layer of control over iron homeostasis.

HFE mutations can lead to hereditary hemochromatosis a condition characterized by excessive iron accumulation in tissues which leads to organ damage. A common mutation is the C282Y mutation which disrupts the protein's normal function. This disorder links to other proteins related to iron metabolism such as hepcidin. Proper functioning of HFE is essential to avoid diseases like hemochromatosis as its impairment results in misregulated iron levels leading to adverse health effects.
style
left-column

Specifications

Form

Liquid

style
left-column

General info

Function

Binds to transferrin receptor (TFR) and reduces its affinity for iron-loaded transferrin.

Sequence similarities

Belongs to the MHC class I family.

style
left-column

Product protocols

style
left-column

Target data

Binds to transferrin receptor (TFR) and reduces its affinity for iron-loaded transferrin.
See full target information HFE
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB133639

Anti-HFE antibody [EPR6751(2)]

RabMAb|Recombinant

Western blot - Anti-HFE antibody [EPR6751(2)] (AB133639)

  • 5
  • 2
Primary Antibodies

AB178430

Anti-PAH antibody [EPR12380]

RabMAb|Recombinant|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-PAH antibody [EPR12380] (AB178430)

  • 0
  • 0
Primary Antibodies

AB207303

Anti-alpha 1 Antitrypsin antibody [EPR17087-50]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Antitrypsin antibody [EPR17087-50] (AB207303)

  • 4
  • 2
Assay Kits

AB241000

Phenylalanine Assay Kit (Colorimetric)

Functional Studies - Phenylalanine Assay Kit (Colorimetric) (AB241000)

  • 5
  • 1

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com