Recombinant human HGF protein (Active) is a Human Full Length protein, in the 32 to 728 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, HPLC, Cell Culture.
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Key facts
- Purity
- >=95% SDS-PAGE
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS, MS, HPLC, Cell Culture
- Biologically active
- Yes
Amino acid sequence
Q R K R R N T I H E F K K S A K T T L I K I D P A L K I K T K K V N T A D Q C A N R C T R N K G L P F T C K A F V F D K A R K Q C L W F P F N S M S S G V K K E F G H E F D L Y E N K D Y I R N C I I G K G R S Y K G T V S I T K S G I K C Q P W S S M I P H E H S F L P S S Y R G K D L Q E N Y C R N P R G E E G G P W C F T S N P E V R Y E V C D I P Q C S E V E C M T C N G E S Y R G L M D H T E S G K I C Q R W D H Q T P H R H K F L P E R Y P D K G F D D N Y C R N P D G Q P R P W C Y T L D P H T R W E Y C A I K T C A D N T M N D T D V P L E T T E C I Q G Q G E G Y R G T V N T I W N G I P C Q R W D S Q Y P H E H D M T P E N F K C K D L R E N Y C R N P D G S E S P W C F T T D P N I R V G Y C S Q I P N C D M S H G Q D C Y R G N G K N Y M G N L S Q T R S G L T C S M W D K N M E D L H R H I F W E P D A S K L N E N Y C R N P D D D A H G P W C Y T G N P L I P W D Y C P I S R C E G D T T P T I V N L D H P V I S C A K T K Q L R V V N G I P T R T N I G W M V S L R Y R N K H I C G G S L I K E S W V L T A R Q C F P S R D L K D Y E A W L G I H D V H G R G D E K C K Q V L N V S Q L V Y G P E G S D L V L M K L A R P A V L D D F V S T I D L P N Y G C T I P E K T S C S V Y G W G Y T G L I N Y D G L L R V A H L Y I M G N E K C S Q H H R G K V T L N E S E I C A G A E K I G S G P C E G D Y G G P L V C E Q H K M R M V L G V I V P G R G C A I P N R P G I F V R V A Y Y A K W I H K I I L T Y K V P Q S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application Cell Culture | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types (PubMed:20624990). Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization (PubMed:15167892, PubMed:20977675). Activates MAPK signaling following TMPRSS13 cleavage and activation (PubMed:20977675).
Alternative names
HPTA, HGF, Hepatocyte growth factor, Hepatopoietin-A, Scatter factor, SF
Recommended products
Recombinant human HGF protein (Active) is a Human Full Length protein, in the 32 to 728 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, HPLC, Cell Culture.
Key facts
- Purity
- >=95% SDS-PAGE
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS, MS, HPLC, Cell Culture
- Biologically active
- Yes
- Biological activity
- Fully biologically active determined by the dose dependent induce IL-11 secretion by Saos‑2 human osteosarcoma cells.
ED50 is ≤4.9 ng/ml, corresponding to a specific activity of 0.20 x 106 units/mg.
- Accession
- P14210-1
- Animal free
- Yes
- Species
- Human
- Reconstitution
- Reconstitute in PBS
- Concentration
- Storage buffer
pH: 6 - 8
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- Q R K R R N T I H E F K K S A K T T L I K I D P A L K I K T K K V N T A D Q C A N R C T R N K G L P F T C K A F V F D K A R K Q C L W F P F N S M S S G V K K E F G H E F D L Y E N K D Y I R N C I I G K G R S Y K G T V S I T K S G I K C Q P W S S M I P H E H S F L P S S Y R G K D L Q E N Y C R N P R G E E G G P W C F T S N P E V R Y E V C D I P Q C S E V E C M T C N G E S Y R G L M D H T E S G K I C Q R W D H Q T P H R H K F L P E R Y P D K G F D D N Y C R N P D G Q P R P W C Y T L D P H T R W E Y C A I K T C A D N T M N D T D V P L E T T E C I Q G Q G E G Y R G T V N T I W N G I P C Q R W D S Q Y P H E H D M T P E N F K C K D L R E N Y C R N P D G S E S P W C F T T D P N I R V G Y C S Q I P N C D M S H G Q D C Y R G N G K N Y M G N L S Q T R S G L T C S M W D K N M E D L H R H I F W E P D A S K L N E N Y C R N P D D D A H G P W C Y T G N P L I P W D Y C P I S R C E G D T T P T I V N L D H P V I S C A K T K Q L R V V N G I P T R T N I G W M V S L R Y R N K H I C G G S L I K E S W V L T A R Q C F P S R D L K D Y E A W L G I H D V H G R G D E K C K Q V L N V S Q L V Y G P E G S D L V L M K L A R P A V L D D F V S T I D L P N Y G C T I P E K T S C S V Y G W G Y T G L I N Y D G L L R V A H L Y I M G N E K C S Q H H R G K V T L N E S E I C A G A E K I G S G P C E G D Y G G P L V C E Q H K M R M V L G V I V P G R G C A I P N R P G I F V R V A Y Y A K W I H K I I L T Y K V P Q S
- Accession
- P14210
- Protein length
- Full Length
- Predicted molecular weight
- 79.72 kDa
- Actual molecular weight
- 79.73 kDa
- Amino acids
- 32 to 728
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
- Additional notes
=95% by HPLC
General info
- Function
Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types (PubMed:20624990). Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization (PubMed:15167892, PubMed:20977675). Activates MAPK signaling following TMPRSS13 cleavage and activation (PubMed:20977675).
- Sequence similarities
Belongs to the peptidase S1 family. Plasminogen subfamily.
- Post-translational modifications
The single-chain precursor undergoes proteolytic processing by TMPRSS13 resulting in an active two-chain form.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The hepatocyte growth factor (HGF) also known as scatter factor (SF) is a protein with a mass of approximately 83 kDa. This protein is mostly expressed in the liver but it can also be found in other organs such as the kidney and the lungs. HGF acts mechanically by binding to the c-Met receptor activating intracellular signaling mechanisms necessary for various physiological processes. Researchers often use HGF ELISA kits to measure its levels in biological samples and study its various roles.
- Biological function summary
HGF plays an essential role in cellular growth regeneration and healing processes across different tissues. It is not part of a complex but functions as a single-chain polypeptide that undergoes cleavage into its active form. HGF facilitates cell proliferation and migration; this is especially important during organ regeneration and repair processes. By engaging with its receptor c-Met it induces cell survival and cytoskeletal reorganization making it important for wound healing.
- Pathways
HGF is critical in the MAPK and PI3K-Akt signaling pathways which impact processes like cell division and survival. Through these pathways HGF interacts with other proteins such as ERK and Akt contributing to the regulation of cellular growth and metabolism. HGF's role in these pathways highlights its importance in developmental processes and tissue homeostasis.
- Associated diseases and disorders
HGF displays significant connections to cancer and liver cirrhosis. Its overexpression can lead to the development of various carcinomas due to its effect on cell proliferation and migration. In liver cirrhosis the altered HGF-c-Met signaling might contribute to the disease progression and hepatic dysfunction. Additionally HGF can interact with proteins such as transforming growth factor-beta (TGF-β) in these conditions influencing the pathological environment.
Product promise
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Terms & Conditions.
4 product images
Functional Studies - Recombinant human HGF protein (Active) (ab259401)
Fully biologically active determined by the dose dependent induce IL-11 secretion by Saos‑2 human osteosarcoma cells.
ED50is ≤4.9 ng/ml, corresponding to a specific activity of 0.20 x 106 units/mg.
Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.
Lot: GR3369519-1
SDS-PAGE - Recombinant human HGF protein (Active) (ab259401)
SDS-PAGE analysis of ab259401.
Additional lower MW bands are result of protein degradation during the production process and do not affect protein function.
Mass Spectrometry - Recombinant human HGF protein (Active) (ab259401)
Mass determination by ESI TOF.
Predicted mass is 79720.38 Da (+/- 10 Da by ESI TOF). Observed mass is 79724.93.
HPLC - Recombinant human HGF protein (Active) (ab259401)
HPLC analysis of ab259401.
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