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AB632

Recombinant human HGF protein (Active)

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(1 Publication)

Recombinant human HGF protein (Active) is a Human Full Length protein, in the 32 to 728 aa range, expressed in BTI-TN-5B1-4 cells, with >98%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS, HPLC.

View Alternative Names

HPTA, HGF, Hepatocyte growth factor, Hepatopoietin-A, Scatter factor, SF

1 Images
Sandwich ELISA - Recombinant human HGF protein (Active) (AB632)
  • sELISA

Unknown

Sandwich ELISA - Recombinant human HGF protein (Active) (AB632)

Standard curve for HGF (Analyte : ab632); dilution range 1pg/ml to 1μg/ml using Capture Antibody ab10829 at 5μg/ml and Detector Antibody ab24865 at 0.5μg/ml.

Key facts

Purity

>98% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

BTI-TN-5B1-4 cells

Tags

Tag free

Applications

FuncS, HPLC, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Determined by the dose-dependent stimulation of the proliferation of monkey 4MBr-5 cells. The expected ED50 for this effect is 1.0-20.0 ng/ml.

Accession

P14210

Animal free

No

Carrier free

Yes

Species

Human

Reconstitution

Reconstitute at 0.5 mg/mL in water

Storage buffer

Constituents: 1.74% DL-Arginine, 1.16% Sodium chloride, 0.12% Tris

storage-buffer

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Complementary Products

Primary Antibodies

AB178395

Anti-HGF antibody [EPR12230]

RabMAb|Recombinant

Immunoprecipitation - Anti-HGF antibody [EPR12230] (AB178395)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Proteins & Peptides

AB280943

Recombinant human PD-L1 protein (Active)

Premium Bioactive

Sandwich ELISA - Recombinant human PD-L1 protein (Active) (AB280943)

  • 0
  • 0
Primary Antibodies

AB205921

Anti-PD-L1 antibody [28-8]

BOND RX™ Validated|20ul selling size|Recombinant|KO Validated|Advanced Validation|RabMAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [28-8] (AB205921)

  • 4
  • 15
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Expression System = Baculovirus infected High-5 cells.
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Sequence info

[{"sequence":"QRKRRNTIHEFKKSAKTTLIKIDPALKIKTKKVNTADQCANRCTRNKGLPFTCKAFVFDKARKQCLWFPFNSMSSGVKKEFGHEFDLYENKDYIRNCIIGKGRSYKGTVSITKSGIKCQPWSSMIPHEHSFLPSSYRGKDLQENYCRNPRGEEGGPWCFTSNPEVRYEVCDIPQCSEVECMTCNGESYRGLMDHTESGKICQRWDHQTPHRHKFLPERYPDKGFDDNYCRNPDGQPRPWCYTLDPHTRWEYCAIKTCADNTMNDTDVPLETTECIQGQGEGYRGTVNTIWNGIPCQRWDSQYPHEHDMTPENFKCKDLRENYCRNPDGSESPWCFTTDPNIRVGYCSQIPNCDMSHGQDCYRGNGKNYMGNLSQTRSGLTCSMWDKNMEDLHRHIFWEPDASKLNENYCRNPDDDAHGPWCYTGNPLIPWDYCPISRCEGDTTPTIVNLDHPVISCAKTKQLRVVNGIPTRTNIGWMVSLRYRNKHICGGSLIKESWVLTARQCFPSRDLKDYEAWLGIHDVHGRGDEKCKQVLNVSQLVYGPEGSDLVLMKLARPAVLDDFVSTIDLPNYGCTIPEKTSCSVYGWGYTGLINYDGLLRVAHLYIMGNEKCSQHHRGKVTLNESEICAGAEKIGSGPCEGDYGGPLVCEQHKMRMVLGVIVPGRGCAIPNRPGIFVRVAYYAKWIHKIILTYKVPQS","proteinLength":"Full Length","predictedMolecularWeight":"80 kDa","actualMolecularWeight":null,"aminoAcidEnd":728,"aminoAcidStart":32,"nature":"Recombinant","expressionSystem":"BTI-TN-5B1-4 cells","accessionNumber":"P14210","tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The hepatocyte growth factor (HGF) also known as scatter factor (SF) is a protein with a mass of approximately 83 kDa. This protein is mostly expressed in the liver but it can also be found in other organs such as the kidney and the lungs. HGF acts mechanically by binding to the c-Met receptor activating intracellular signaling mechanisms necessary for various physiological processes. Researchers often use HGF ELISA kits to measure its levels in biological samples and study its various roles.
Biological function summary

HGF plays an essential role in cellular growth regeneration and healing processes across different tissues. It is not part of a complex but functions as a single-chain polypeptide that undergoes cleavage into its active form. HGF facilitates cell proliferation and migration; this is especially important during organ regeneration and repair processes. By engaging with its receptor c-Met it induces cell survival and cytoskeletal reorganization making it important for wound healing.

Pathways

HGF is critical in the MAPK and PI3K-Akt signaling pathways which impact processes like cell division and survival. Through these pathways HGF interacts with other proteins such as ERK and Akt contributing to the regulation of cellular growth and metabolism. HGF's role in these pathways highlights its importance in developmental processes and tissue homeostasis.

HGF displays significant connections to cancer and liver cirrhosis. Its overexpression can lead to the development of various carcinomas due to its effect on cell proliferation and migration. In liver cirrhosis the altered HGF-c-Met signaling might contribute to the disease progression and hepatic dysfunction. Additionally HGF can interact with proteins such as transforming growth factor-beta (TGF-β) in these conditions influencing the pathological environment.
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Specifications

Form

Lyophilized

Additional notes

>=98% HPLC analyses.Sterile filtered.

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General info

Function

Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types (PubMed : 20624990). Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization (PubMed : 15167892, PubMed : 20977675). Activates MAPK signaling following TMPRSS13 cleavage and activation (PubMed : 20977675).

Sequence similarities

Belongs to the peptidase S1 family. Plasminogen subfamily.

Post-translational modifications

The single-chain precursor undergoes proteolytic processing by TMPRSS13 resulting in an active two-chain form (PubMed:20977675). The single-chain precursor undergoes proteolytic processing by HGFAC resulting in an active two-chain form (PubMed:8226803).

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Product protocols

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Target data

Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types (PubMed : 20624990). Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization (PubMed : 15167892, PubMed : 20977675). Activates MAPK signaling following TMPRSS13 cleavage and activation (PubMed : 20977675).
See full target information HGF
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell stem cell 29:1315-1332.e9 PubMed35998642

2022

The mitochondrial protein OPA1 regulates the quiescent state of adult muscle stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Nicole Baker,Steven Wade,Matthew Triolo,John Girgis,Damian Chwastek,Sarah Larrigan,Peter Feige,Ryo Fujita,Colin Crist,Michael A Rudnicki,Yan Burelle,Mireille Khacho
View all publications
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