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Recombinant human HGF protein (Active) is a Human Full Length protein, in the 32 to 728 aa range, expressed in BTI-TN-5B1-4, with >=98% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, HPLC.

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Key facts

Purity

>=98% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

BTI-TN-5B1-4 cells

Tags

Tag free

Applications

SDS-PAGE, FuncS, HPLC

Biologically active

Yes

Amino acid sequence

Q R K R R N T I H E F K K S A K T T L I K I D P A L K I K T K K V N T A D Q C A N R C T R N K G L P F T C K A F V F D K A R K Q C L W F P F N S M S S G V K K E F G H E F D L Y E N K D Y I R N C I I G K G R S Y K G T V S I T K S G I K C Q P W S S M I P H E H S F L P S S Y R G K D L Q E N Y C R N P R G E E G G P W C F T S N P E V R Y E V C D I P Q C S E V E C M T C N G E S Y R G L M D H T E S G K I C Q R W D H Q T P H R H K F L P E R Y P D K G F D D N Y C R N P D G Q P R P W C Y T L D P H T R W E Y C A I K T C A D N T M N D T D V P L E T T E C I Q G Q G E G Y R G T V N T I W N G I P C Q R W D S Q Y P H E H D M T P E N F K C K D L R E N Y C R N P D G S E S P W C F T T D P N I R V G Y C S Q I P N C D M S H G Q D C Y R G N G K N Y M G N L S Q T R S G L T C S M W D K N M E D L H R H I F W E P D A S K L N E N Y C R N P D D D A H G P W C Y T G N P L I P W D Y C P I S R C E G D T T P T I V N L D H P V I S C A K T K Q L R V V N G I P T R T N I G W M V S L R Y R N K H I C G G S L I K E S W V L T A R Q C F P S R D L K D Y E A W L G I H D V H G R G D E K C K Q V L N V S Q L V Y G P E G S D L V L M K L A R P A V L D D F V S T I D L P N Y G C T I P E K T S C S V Y G W G Y T G L I N Y D G L L R V A H L Y I M G N E K C S Q H H R G K V T L N E S E I C A G A E K I G S G P C E G D Y G G P L V C E Q H K M R M V L G V I V P G R G C A I P N R P G I F V R V A Y Y A K W I H K I I L T Y K V P Q S

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Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Application

FuncS

Reactivity

Reacts

Dilution info

-

Notes

-

Application

HPLC

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization.

Alternative names

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Recombinant human HGF protein (Active) is a Human Full Length protein, in the 32 to 728 aa range, expressed in BTI-TN-5B1-4, with >=98% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, HPLC.

Alternative names

Key facts

Purity

>=98% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

BTI-TN-5B1-4 cells

Applications

SDS-PAGE, FuncS, HPLC

Biological activity

Determined by the dose-dependent stimulation of the proliferation of monkey 4MBr-5 cells. The expected ED50 for this effect is 1.0-20.0 ng/ml.

Accession
P14210-1
Animal free

No

Carrier free

Yes

Species

Human

Reconstitution

Reconstitute at 0.5 mg/mL in water

Concentration
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Storage buffer

Constituents: 1.74% DL-Arginine, 1.16% Sodium chloride, 0.12% Tris

Sequence info

Amino acid sequence

Q R K R R N T I H E F K K S A K T T L I K I D P A L K I K T K K V N T A D Q C A N R C T R N K G L P F T C K A F V F D K A R K Q C L W F P F N S M S S G V K K E F G H E F D L Y E N K D Y I R N C I I G K G R S Y K G T V S I T K S G I K C Q P W S S M I P H E H S F L P S S Y R G K D L Q E N Y C R N P R G E E G G P W C F T S N P E V R Y E V C D I P Q C S E V E C M T C N G E S Y R G L M D H T E S G K I C Q R W D H Q T P H R H K F L P E R Y P D K G F D D N Y C R N P D G Q P R P W C Y T L D P H T R W E Y C A I K T C A D N T M N D T D V P L E T T E C I Q G Q G E G Y R G T V N T I W N G I P C Q R W D S Q Y P H E H D M T P E N F K C K D L R E N Y C R N P D G S E S P W C F T T D P N I R V G Y C S Q I P N C D M S H G Q D C Y R G N G K N Y M G N L S Q T R S G L T C S M W D K N M E D L H R H I F W E P D A S K L N E N Y C R N P D D D A H G P W C Y T G N P L I P W D Y C P I S R C E G D T T P T I V N L D H P V I S C A K T K Q L R V V N G I P T R T N I G W M V S L R Y R N K H I C G G S L I K E S W V L T A R Q C F P S R D L K D Y E A W L G I H D V H G R G D E K C K Q V L N V S Q L V Y G P E G S D L V L M K L A R P A V L D D F V S T I D L P N Y G C T I P E K T S C S V Y G W G Y T G L I N Y D G L L R V A H L Y I M G N E K C S Q H H R G K V T L N E S E I C A G A E K I G S G P C E G D Y G G P L V C E Q H K M R M V L G V I V P G R G C A I P N R P G I F V R V A Y Y A K W I H K I I L T Y K V P Q S

Accession

P14210

Protein length

Full Length

Predicted molecular weight

80 kDa

Amino acids

32 to 728

Nature

Recombinant

Specifications

Form

Lyophilized

Additional notes

>=98% HPLC analyses.Sterile filtered.

General info

Function

Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization.

Sequence similarities

Belongs to the peptidase S1 family. Plasminogen subfamily.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Notes

Expression System = Baculovirus infected High-5 cells.

Supplementary info

Biological function summary

HGF plays an essential role in cellular growth regeneration and healing processes across different tissues. It is not part of a complex but functions as a single-chain polypeptide that undergoes cleavage into its active form. HGF facilitates cell proliferation and migration; this is especially important during organ regeneration and repair processes. By engaging with its receptor c-Met it induces cell survival and cytoskeletal reorganization making it important for wound healing.

Activity summary

The hepatocyte growth factor (HGF) also known as scatter factor (SF) is a protein with a mass of approximately 83 kDa. This protein is mostly expressed in the liver but it can also be found in other organs such as the kidney and the lungs. HGF acts mechanically by binding to the c-Met receptor activating intracellular signaling mechanisms necessary for various physiological processes. Researchers often use HGF ELISA kits to measure its levels in biological samples and study its various roles.

Pathways

HGF is critical in the MAPK and PI3K-Akt signaling pathways which impact processes like cell division and survival. Through these pathways HGF interacts with other proteins such as ERK and Akt contributing to the regulation of cellular growth and metabolism. HGF's role in these pathways highlights its importance in developmental processes and tissue homeostasis.

Associated diseases and disorders

HGF displays significant connections to cancer and liver cirrhosis. Its overexpression can lead to the development of various carcinomas due to its effect on cell proliferation and migration. In liver cirrhosis the altered HGF-c-Met signaling might contribute to the disease progression and hepatic dysfunction. Additionally HGF can interact with proteins such as transforming growth factor-beta (TGF-β) in these conditions influencing the pathological environment.

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1 product image

  • Sandwich ELISA - Recombinant human HGF protein (Active) (ab632), expandable thumbnail

    Sandwich ELISA - Recombinant human HGF protein (Active) (ab632)

    Standard curve for HGF (Analyte: ab632); dilution range 1pg/ml to 1μg/ml using Capture Antibody ab10829 at 5μg/ml and Detector Antibody ab24865 at 0.5μg/ml.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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