Recombinant Human hHR23A protein is a Human Full Length protein, in the 1 to 363 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Images
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
M A V T I T L K T L Q Q Q T F K I R M E P D E T V K V L K E K I E A E K G R D A F P V A G Q K L I Y A G K I L S D D V P I R D Y R I D E K N F V V V M V T K T K A G Q G T S A P P E A S P T A A P E S S T S F P P A P T S G M S H P P P A A R E D K S P S E E S A P T T S P E S V S G S V P S S G S S G R E E D A A S T L V T G S E Y E T M L T E I M S M G Y E R E R V V A A L R A S Y N N P H R A V E Y L L T G I P G S P E P E H G S V Q E S Q V S E Q P A T E A A G E N P L E F L R D Q P Q F Q N M R Q V I Q Q N P A L L P A L L Q Q L G Q E N P Q L L Q Q I S R H Q E Q F I Q M L N E P P G E L A D I S D V E G E V G A I G E E A P Q M N Y I Q V T P Q E K E A I E R L K A L G F P E S L V I Q A Y F A C E K N E N L A A N F L L S Q N F D D E
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Binds to 'Lys-48'-linked polyubiquitin chains in a length-dependent manner and with a lower affinity to 'Lys-63'-linked polyubiquitin chains. Proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome. Involved in nucleotide excision repair and is thought to be functional equivalent for RAD23B in global genome nucleotide excision repair (GG-NER) by association with XPC. In vitro, the XPC:RAD23A dimer has NER activity. Can stabilize XPC. (Microbial infection) Involved in Vpr-dependent replication of HIV-1 in non-proliferating cells and primary macrophages. Required for the association of HIV-1 Vpr with the host proteasome.
Alternative names
UV excision repair protein RAD23 homolog A, HR23A, hHR23A, RAD23A
Recommended products
Recombinant Human hHR23A protein is a Human Full Length protein, in the 1 to 363 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- P54725-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M A V T I T L K T L Q Q Q T F K I R M E P D E T V K V L K E K I E A E K G R D A F P V A G Q K L I Y A G K I L S D D V P I R D Y R I D E K N F V V V M V T K T K A G Q G T S A P P E A S P T A A P E S S T S F P P A P T S G M S H P P P A A R E D K S P S E E S A P T T S P E S V S G S V P S S G S S G R E E D A A S T L V T G S E Y E T M L T E I M S M G Y E R E R V V A A L R A S Y N N P H R A V E Y L L T G I P G S P E P E H G S V Q E S Q V S E Q P A T E A A G E N P L E F L R D Q P Q F Q N M R Q V I Q Q N P A L L P A L L Q Q L G Q E N P Q L L Q Q I S R H Q E Q F I Q M L N E P P G E L A D I S D V E G E V G A I G E E A P Q M N Y I Q V T P Q E K E A I E R L K A L G F P E S L V I Q A Y F A C E K N E N L A A N F L L S Q N F D D E
- Accession
- P54725
- Protein length
- Full Length
- Predicted molecular weight
- 66 kDa
- Amino acids
- 1 to 363
- Nature
- Recombinant
Specifications
- Form
- Liquid
General info
- Function
Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Binds to 'Lys-48'-linked polyubiquitin chains in a length-dependent manner and with a lower affinity to 'Lys-63'-linked polyubiquitin chains. Proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome.
- Sequence similarities
Belongs to the RAD23 family.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The human homolog of Rad23A or hHR23A is an important player in protein maintenance and degradation pathways. This protein is known for its involvement in DNA repair mechanisms and proteasome function. hHR23A weighs approximately 43 kDa and expresses in many tissues reflecting its diverse roles throughout the body. Scientists also refer to it as RAD23A giving insight into its function by linking with the well-known RAD proteins.
- Biological function summary
HHR23A contributes significantly to the protein ubiquitination process an essential cellular mechanism for targeting proteins for degradation. It forms part of a complex with ubiquitin aiding in the recognition and delivery of ubiquitinated proteins to the proteasome for degradation. The protein's ability to bind to both ubiquitin and the proteasome enables its dual function in proteolytic pathways positioning hHR23A as an intermediary that regulates protein turnover and stability.
- Pathways
HHR23A engages in the ubiquitin-proteasome pathway and the nucleotide excision repair (NER) pathway. It cooperates with other proteins like Rad4 (XPC in humans) in NER where it functions in DNA damage recognition and repair. In the ubiquitin-proteasome pathway hHR23A interacts with the proteasomal subunit Rpn10 and other ubiquitin-like proteins. These interactions facilitate effective proteolysis of damaged or unnecessary proteins emphasizing its central role in controlling intracellular protein levels.
- Associated diseases and disorders
HHR23A has implications in neurodegenerative diseases and cancer. Impaired ubiquitin-proteasome system function involving hHR23A associates with neurodegenerative diseases such as Alzheimer's where protein aggregates accumulate. hHR23A's interaction with proteins like p53 known for its role in tumor suppression ties this protein to cancer biology highlighting its potential involvement in tumor suppression failure when its pathways are disrupted.
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1 product image
SDS-PAGE - Recombinant Human hHR23A protein (ab152648)
12.5% SDS-PAGE analysis of ab152648 stained with Coomassie Blue.
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