Recombinant Human HIF-1 alpha protein is a Human Full Length protein, in the 1 to 735 aa range, expressed in Escherichia coli, with >75% purity and suitable for SDS-PAGE, ELISA, WB.
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- International journal of molecular sciences 25:2024The Role of Hypoxia-Inducible Factor 1 Alpha in Acute-on-Chronic Liver Failure.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarcus M Mücke,Nihad El Bali,Katharina M Schwarzkopf,Frank Erhard Uschner,Nico Kraus,Larissa Eberle,Victoria Therese Mücke,Julia Bein,Sandra Beyer,Peter J Wild,Robert Schierwagen,Sabine Klein,Stefan Zeuzem,Christoph Welsch,Jonel Trebicka,Angela BriegerPubMed 38338821
- PLoS biology 18:e30006602020HIV-1 Tat-mediated astrocytic amyloidosis involves the HIF-1α/lncRNA BACE1-AS axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSusmita Sil et. al.PubMed 32453744
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 34:4293-43102020Regulation of hypoxia-inducible factor functions in the nucleus by sphingosine-1-phosphate.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNitai C Hait,Aparna Maiti,Pan Xu,Qianya Qi,Tsutomu Kawaguchi,Maiko Okano,Kazuaki Takabe,Li Yan,Cheng LuoPubMed 32017264
- Cells 9:2020The Reverse Warburg Effect is Associated with Fbp2-Dependent Hif1α Regulation in Cancer Cells Stimulated by Fibroblasts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPrzemysław Duda et. al.PubMed 31947613
- Fertility and sterility 105:1496-1502.e42016Hypoxia inducible factor and microvessels in peri-implantation endometrium of women with recurrent miscarriage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoyan Chen et. al.PubMed 27018158
Key facts
- Purity
- >75% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
M S D K I I H L T D D S F D T D V L K A D G A I L V D F W A E W C G P C K M I A P I L D E I A D E Y Q G K L T V A K L N I D Q N P G T A P K Y G I R G I P T L L L F K N G E V A A T K V G A L S K G Q L K E F L D A N L A G S G S G H M H H H H H H S S G L V P R G S G M K E T A A A K F E R Q H M D S P D L G T E N L Y F Q G M E G A G G A N D K K K I S S E R R K E K S R D A A R S R R S K E S E V F Y E L A H Q L P L P H N V S S H L D K A S V M R L T I S Y L R V R K L L D A G D L D I E D D M K A Q M N C F Y L K A L D G F V M V L T D D G D M I Y I S D N V N K Y M G L T Q F E L T G H S V F D F T H P C D H E E M R E M L T H R N G L V K K G K E Q N T Q R S F F L R M K C T L T S R G R T M N I K S A T W K V L H C T G H I H V Y D T N S N Q P Q C G Y K K P P M T C L V L I C E P I P H P S N I E I P L D S K T F L S R H S L D M K F S Y C D E R I T E L M G Y E P E E L L G R S I Y E Y Y H A L D S D H L T K T H H D M F T K G Q V T T G Q Y R M L A K R G G Y V W V E T Q A T V I Y N T K N S Q P Q C I V C V N Y V V S G I I Q H D L I F S L Q Q T E C V L K P V E S S D M K M T Q L F T K V E S E D T S S L F D K L K K E P D A L T L L A P A A G D T I I S L D F G S N D T E T D D Q Q L E E V P L Y N D V M L P S P N E K L Q N I N L A M S P L P T A E T P K P L R S S A D P A L N Q E V A L K L E P N P E S L E L S F T M P Q I Q D Q T P S P S D G S T R Q S S P E P N S P S E Y C F Y V D S D M V N E F K L E L V E K L F A E D T E A K N P F S T Q D T D L D L E M L A P Y I P M D D D F Q L R S F D Q L S P L E S S S A S P E S A S P Q S T V T V F Q Q T Q I Q E P T A N A T T T T A T T D E L K T V T K D R M E D I K I L I A S P S P T H I H K E T T S A T S S P Y R D T Q S R T A S P N R A G K G V I E Q T E K S H P R S P N V L S V A L S Q R T T V P E E E L N P K I L A L Q N A Q R K R K M E H D G S L F Q A V G I G
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Functions as a master transcriptional regulator of the adaptive response to hypoxia (PubMed:11292861, PubMed:11566883, PubMed:15465032, PubMed:16973622, PubMed:17610843, PubMed:18658046, PubMed:20624928, PubMed:22009797, PubMed:30125331, PubMed:9887100). Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia (PubMed:11292861, PubMed:11566883, PubMed:15465032, PubMed:16973622, PubMed:17610843, PubMed:20624928, PubMed:22009797, PubMed:30125331, PubMed:9887100). Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease (PubMed:22009797). Heterodimerizes with ARNT; heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters (By similarity). Activation requires recruitment of transcriptional coactivators such as CREBBP and EP300 (PubMed:16543236, PubMed:9887100). Activity is enhanced by interaction with NCOA1 and/or NCOA2 (PubMed:10594042). Interaction with redox regulatory protein APEX1 seems to activate CTAD and potentiates activation by NCOA1 and CREBBP (PubMed:10202154, PubMed:10594042). Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia (PubMed:19528298). (Microbial infection) Upon infection by human coronavirus SARS-CoV-2, is required for induction of glycolysis in monocytes and the consequent pro-inflammatory state (PubMed:32697943). In monocytes, induces expression of ACE2 and cytokines such as IL1B, TNF, IL6, and interferons (PubMed:32697943). Promotes human coronavirus SARS-CoV-2 replication and monocyte inflammatory response (PubMed:32697943).
Alternative names
BHLHE78, MOP1, PASD8, HIF1A, Hypoxia-inducible factor 1-alpha, HIF-1-alpha, HIF1-alpha, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78, Member of PAS protein 1, PAS domain-containing protein 8, bHLHe78
Recommended products
Recombinant Human HIF-1 alpha protein is a Human Full Length protein, in the 1 to 735 aa range, expressed in Escherichia coli, with >75% purity and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Purity
- >75% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- Q16665-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.61% Tris
Sequence info
Amino acid sequence
- M S D K I I H L T D D S F D T D V L K A D G A I L V D F W A E W C G P C K M I A P I L D E I A D E Y Q G K L T V A K L N I D Q N P G T A P K Y G I R G I P T L L L F K N G E V A A T K V G A L S K G Q L K E F L D A N L A G S G S G H M H H H H H H S S G L V P R G S G M K E T A A A K F E R Q H M D S P D L G T E N L Y F Q G M E G A G G A N D K K K I S S E R R K E K S R D A A R S R R S K E S E V F Y E L A H Q L P L P H N V S S H L D K A S V M R L T I S Y L R V R K L L D A G D L D I E D D M K A Q M N C F Y L K A L D G F V M V L T D D G D M I Y I S D N V N K Y M G L T Q F E L T G H S V F D F T H P C D H E E M R E M L T H R N G L V K K G K E Q N T Q R S F F L R M K C T L T S R G R T M N I K S A T W K V L H C T G H I H V Y D T N S N Q P Q C G Y K K P P M T C L V L I C E P I P H P S N I E I P L D S K T F L S R H S L D M K F S Y C D E R I T E L M G Y E P E E L L G R S I Y E Y Y H A L D S D H L T K T H H D M F T K G Q V T T G Q Y R M L A K R G G Y V W V E T Q A T V I Y N T K N S Q P Q C I V C V N Y V V S G I I Q H D L I F S L Q Q T E C V L K P V E S S D M K M T Q L F T K V E S E D T S S L F D K L K K E P D A L T L L A P A A G D T I I S L D F G S N D T E T D D Q Q L E E V P L Y N D V M L P S P N E K L Q N I N L A M S P L P T A E T P K P L R S S A D P A L N Q E V A L K L E P N P E S L E L S F T M P Q I Q D Q T P S P S D G S T R Q S S P E P N S P S E Y C F Y V D S D M V N E F K L E L V E K L F A E D T E A K N P F S T Q D T D L D L E M L A P Y I P M D D D F Q L R S F D Q L S P L E S S S A S P E S A S P Q S T V T V F Q Q T Q I Q E P T A N A T T T T A T T D E L K T V T K D R M E D I K I L I A S P S P T H I H K E T T S A T S S P Y R D T Q S R T A S P N R A G K G V I E Q T E K S H P R S P N V L S V A L S Q R T T V P E E E L N P K I L A L Q N A Q R K R K M E H D G S L F Q A V G I G
- Accession
- Q16665
- Protein length
- Full Length
- Predicted molecular weight
- 100 kDa
- Amino acids
- 1 to 735
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab154478 was purified by Ni chromatography and sterile filtered.
General info
- Function
Functions as a master transcriptional regulator of the adaptive response to hypoxia (PubMed:11292861, PubMed:11566883, PubMed:15465032, PubMed:16973622, PubMed:17610843, PubMed:18658046, PubMed:20624928, PubMed:22009797, PubMed:30125331, PubMed:9887100). Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia (PubMed:11292861, PubMed:11566883, PubMed:15465032, PubMed:16973622, PubMed:17610843, PubMed:20624928, PubMed:22009797, PubMed:30125331, PubMed:9887100). Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease (PubMed:22009797). Heterodimerizes with ARNT; heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters (By similarity). Activation requires recruitment of transcriptional coactivators such as CREBBP and EP300 (PubMed:16543236, PubMed:9887100). Activity is enhanced by interaction with NCOA1 and/or NCOA2 (PubMed:10594042). Interaction with redox regulatory protein APEX1 seems to activate CTAD and potentiates activation by NCOA1 and CREBBP (PubMed:10202154, PubMed:10594042). Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia (PubMed:19528298).
- Post-translational modifications
S-nitrosylation of Cys-800 may be responsible for increased recruitment of p300 coactivator necessary for transcriptional activity of HIF-1 complex.
- Subcellular localisation
- Nucleus, Nucleus speckle
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
Notes
Product was previously marketed under the MitoSciences sub-brand.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
HIF-1 alpha also known as hypoxia-inducible factor 1-alpha is a transcription factor critical in cellular response to low oxygen levels. Its molecular weight usually ranges from 93 to 120 kDa. You can find HIF-1 alpha expressed in tissues throughout the body but its expression significantly increases under hypoxic conditions. Researchers often use the HIF-1a ELISA to measure its expression levels. HIF-1 alpha forms a complex with other proteins to perform its functions effectively.
- Biological function summary
HIF-1 alpha regulates gene expression in response to hypoxic conditions in cells. It forms a complex with HIF-1 beta to activate transcription of various genes involved in energy metabolism angiogenesis and erythropoiesis. HIF-1 alpha enables cells to adapt to reduced oxygen availability allowing for cellular survival and function under stress. It plays an important role in promoting the expression of genes like VEGF and EPO which are important for vascular and red blood cell development respectively.
- Pathways
HIF-1 alpha plays an integral role in the hypoxia signaling pathway and the glycolytic pathway. In the hypoxia signaling pathway HIF-1 alpha partners with VHL (Von Hippel-Lindau) protein that regulates its degradation under normal oxygen conditions. When oxygen levels drop HIF-1 alpha avoids degradation stabilizes and translocates into the nucleus to initiate transcription of hypoxia-responsive genes. The glycolytic pathway involvement highlights its function in adapting energy production under hypoxic conditions through collaboration with enzymes and transporters associated with glycolysis.
- Associated diseases and disorders
HIF-1 alpha has been implicated in cancer and ischemic diseases. Its role in promoting angiogenesis and metabolic adaptation makes it a contributor to tumor growth and survival collaborating with oncogenes such as c-Myc. In ischemic diseases like stroke or myocardial infarction HIF-1 alpha's ability to induce protective responses can mitigate tissue damage through regulation of survival pathways. Understanding these interactions helps in the development of therapeutic strategies targeting HIF-1 alpha in disease contexts.
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4 product images
SDS-PAGE - Recombinant Human HIF-1 alpha protein (ab154478)
1μg of ab154478 was examined by SDS-PAGE and commassie blue protein stain. Full-length HIF-1-alpha is indicated by the arrow. Purity is judged to be >75%.
Expected MW is 100kDa.
Western blot - Recombinant Human HIF-1 alpha protein (ab154478)
We recommend using 5% milk in TBST as the blocking agent, decreasing to 2% milk in TBST during primary and secondary antibody incubation.
Blots were developed with Goat Anti-Mouse IgG H&L (HRP) preadsorbed (Goat Anti-Mouse IgG H&L (HRP) preadsorbed ab97040) secondary antibodyAll lanes: Western blot - Anti-HIF-1 alpha antibody [H1alpha67] (Anti-HIF-1 alpha antibody [H1alpha67] ab1) at 5 µg/mL
Lane 1: Western blot - HeLa nuclear extract lysate (HeLa nuclear extract lysate ab150036) at 40 µg
Lane 2: Western blot - Hela-DFO treated (0.5mM, 24h) Nuclear Lysate (Hela-DFO treated (0.5mM, 24h) Nuclear Lysate ab180880) at 40 µg
Lane 3: HeLa nuclear control at 40 µg
Lane 4: HeLa nuclear DFO treated at 40 µg
Lane 5: Western blot - Recombinant Human HIF-1 alpha protein (ab154478) at 0.001 µg
SecondaryAll lanes: Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (Goat Anti-Mouse IgG H&L (HRP) preadsorbed ab97040) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 92 kDa
Exposure time: 20min
Western blot - Recombinant Human HIF-1 alpha protein (ab154478)
ab154478 was examined by western blot with an anti-HIF-1-alpha antibody.
Lane1: 10ng HIF1 alpha full-length protein (ab154478)
Block: 4% milk/PBS Primary antibody: anti-HIF-1-alpha (Anti-HIF-1 alpha antibody [EP1215Y] ab51608), 1:1000; 2 hours room temperature. Secondary antibody: anti-Rabbit HRP 1:5000 ECL detection
All lanes: Western blot - Recombinant Human HIF-1 alpha protein (ab154478)
ELISA - Recombinant Human HIF-1 alpha protein (ab154478)
ab154478 was tested in the HIF1A Human ELISA Kit (ab117996). ab154478 was tested under standard conditions in the sandwich ELISA kit using a 3-fold dilution series from 3µg/ml.
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