Recombinant Human HIF-1 alpha protein (His-tag) is a Human Fragment protein, in the 575 to 826 aa range, expressed in Escherichia coli, with <95% purity, <= 0.1 EU/mg endotoxin level and suitable for HPLC, SDS-PAGE, MS.
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Key facts
- Purity
- <95% HPLC
- Endotoxin level
- <= 0.1 EU/mg
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- HPLC, SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
R S F D Q L S P L E S S S A S P E S A S P Q S T V T V F Q Q T Q I Q E P T A N A T T T T A T T D E L K T V T K D R M E D I K I L I A S P S P T H I H K E T T S A T S S P Y R D T Q S R T A S P N R A G K G V I E Q T E K S H P R S P N V L S V A L S Q R T T V P E E E L N P K I L A L Q N A Q R K R K M E H D G S L F Q A V G I G T L L Q Q P D D H A A T T S L S W K R V K G C K S S E Q N G M E Q K T I I L I P S D L A C R L L G Q S M D E S G L P Q L T S Y D C E V N A P I Q G S R N L L Q G E E L L R A L D Q V N
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
The protein expressed by the gene HIF1A functions as a master transcriptional regulator of the adaptive response to hypoxia, activating the transcription of over 40 genes under hypoxic conditions, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and others. These genes' protein products enhance oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF1A is crucial for embryonic vascularization, tumor angiogenesis, and ischemic disease pathophysiology. Its activation requires transcriptional coactivators like CREBBP and EP300, with activity enhanced by interactions with NCOA1 and/or NCOA2. Interaction with redox regulatory protein APEX1 activates CTAD and enhances activation by NCOA1 and CREBBP. Additionally, HIF1A is involved in axonal distribution and mitochondrial transport in neurons during hypoxia. In the context of microbial infection, specifically human coronavirus SARS-CoV-2, HIF1A is necessary for glycolysis induction in monocytes, leading to a proinflammatory state, inducing expression of ACE2, cytokines, and promoting virus replication and monocyte inflammatory response. This supplementary information is collated from multiple sources and compiled automatically.
Alternative names
BHLHE78, MOP1, PASD8, HIF1A, Hypoxia-inducible factor 1-alpha, HIF-1-alpha, HIF1-alpha, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78, Member of PAS protein 1, PAS domain-containing protein 8, bHLHe78
Recommended products
Recombinant Human HIF-1 alpha protein (His-tag) is a Human Fragment protein, in the 575 to 826 aa range, expressed in Escherichia coli, with <95% purity, <= 0.1 EU/mg endotoxin level and suitable for HPLC, SDS-PAGE, MS.
Key facts
- Purity
- <95% HPLC
- Endotoxin level
- <= 0.1 EU/mg
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- HPLC, SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q16665-1
- Animal free
- Yes
- Species
- Human
- Reconstitution
- Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product. Store lyophilized form at room temperature. Reconstitute in phosphate buffered saline, aliquot and store at -80°C for 12 months or +4°C for 1 week. Avoid repeated freeze-thaw.
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- R S F D Q L S P L E S S S A S P E S A S P Q S T V T V F Q Q T Q I Q E P T A N A T T T T A T T D E L K T V T K D R M E D I K I L I A S P S P T H I H K E T T S A T S S P Y R D T Q S R T A S P N R A G K G V I E Q T E K S H P R S P N V L S V A L S Q R T T V P E E E L N P K I L A L Q N A Q R K R K M E H D G S L F Q A V G I G T L L Q Q P D D H A A T T S L S W K R V K G C K S S E Q N G M E Q K T I I L I P S D L A C R L L G Q S M D E S G L P Q L T S Y D C E V N A P I Q G S R N L L Q G E E L L R A L D Q V N
- Accession
- Q16665
- Protein length
- Fragment
- Predicted molecular weight
- 28.7 kDa
- Actual molecular weight
- 28.5 kDa
- Amino acids
- 575 to 826
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Lyophilized
- Additional notes
SDS-PAGE < 95%
General info
- Function
The protein expressed by the gene HIF1A functions as a master transcriptional regulator of the adaptive response to hypoxia, activating the transcription of over 40 genes under hypoxic conditions, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and others. These genes' protein products enhance oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF1A is crucial for embryonic vascularization, tumor angiogenesis, and ischemic disease pathophysiology. Its activation requires transcriptional coactivators like CREBBP and EP300, with activity enhanced by interactions with NCOA1 and/or NCOA2. Interaction with redox regulatory protein APEX1 activates CTAD and enhances activation by NCOA1 and CREBBP. Additionally, HIF1A is involved in axonal distribution and mitochondrial transport in neurons during hypoxia. In the context of microbial infection, specifically human coronavirus SARS-CoV-2, HIF1A is necessary for glycolysis induction in monocytes, leading to a proinflammatory state, inducing expression of ACE2, cytokines, and promoting virus replication and monocyte inflammatory response.
This supplementary information is collated from multiple sources and compiled automatically.- Post-translational modifications
S-nitrosylation of Cys-800 may be responsible for increased recruitment of p300 coactivator necessary for transcriptional activity of HIF-1 complex.
- Subcellular localisation
- Nucleus, Nucleus speckle
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
HIF-1 alpha also known as hypoxia-inducible factor 1-alpha is a transcription factor critical in cellular response to low oxygen levels. Its molecular weight usually ranges from 93 to 120 kDa. You can find HIF-1 alpha expressed in tissues throughout the body but its expression significantly increases under hypoxic conditions. Researchers often use the HIF-1a ELISA to measure its expression levels. HIF-1 alpha forms a complex with other proteins to perform its functions effectively.
- Biological function summary
HIF-1 alpha regulates gene expression in response to hypoxic conditions in cells. It forms a complex with HIF-1 beta to activate transcription of various genes involved in energy metabolism angiogenesis and erythropoiesis. HIF-1 alpha enables cells to adapt to reduced oxygen availability allowing for cellular survival and function under stress. It plays an important role in promoting the expression of genes like VEGF and EPO which are important for vascular and red blood cell development respectively.
- Pathways
HIF-1 alpha plays an integral role in the hypoxia signaling pathway and the glycolytic pathway. In the hypoxia signaling pathway HIF-1 alpha partners with VHL (Von Hippel-Lindau) protein that regulates its degradation under normal oxygen conditions. When oxygen levels drop HIF-1 alpha avoids degradation stabilizes and translocates into the nucleus to initiate transcription of hypoxia-responsive genes. The glycolytic pathway involvement highlights its function in adapting energy production under hypoxic conditions through collaboration with enzymes and transporters associated with glycolysis.
- Associated diseases and disorders
HIF-1 alpha has been implicated in cancer and ischemic diseases. Its role in promoting angiogenesis and metabolic adaptation makes it a contributor to tumor growth and survival collaborating with oncogenes such as c-Myc. In ischemic diseases like stroke or myocardial infarction HIF-1 alpha's ability to induce protective responses can mitigate tissue damage through regulation of survival pathways. Understanding these interactions helps in the development of therapeutic strategies targeting HIF-1 alpha in disease contexts.
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2 product images
Mass Spectrometry - Recombinant Human HIF-1 alpha protein (His-tag) (ab309958)
Mass determination by ESI-TOF. Predicted MW is 28663.15 (+/- 10Da by ESI-TOF). Observed MW is 28532.32.
SDS-PAGE - Recombinant Human HIF-1 alpha protein (His-tag) (ab309958)
SDS-PAGE analysis of ab309958
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