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AB157907

Recombinant Human HIF1 beta protein (GST tag N-Terminus)

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Recombinant Human HIF1 beta protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 789 aa range, expressed in Wheat germ, suitable for ELISA, WB.

View Alternative Names

BHLHE2, ARNT, Aryl hydrocarbon receptor nuclear translocator, ARNT protein, Class E basic helix-loop-helix protein 2, Hypoxia-inducible factor 1-beta, bHLHe2, HIF-1-beta, HIF1-beta

1 Images
SDS-PAGE - Recombinant Human HIF1 beta protein (GST tag N-Terminus) (AB157907)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human HIF1 beta protein (GST tag N-Terminus) (AB157907)

ab157907 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

WB, ELISA

applications

Biologically active

No

Accession

P27540

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Complementary Products

Primary Antibodies

AB270520

Anti-HIF1 beta antibody [EPR23106-151]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF1 beta antibody [EPR23106-151] (AB270520)

  • 0
  • 0
Proteins & Peptides

AB152176

Recombinant Human Aryl hydrocarbon Receptor protein (GST tag N-Terminus)

Western blot - Recombinant Human Aryl hydrocarbon Receptor protein (GST tag N-Terminus) (AB152176)

  • 0
  • 0
Primary Antibodies

AB190797

Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] (AB190797)

  • 0
  • 0
Primary Antibodies

AB70122

Anti-ARNT2 antibody

Western blot - Anti-ARNT2 antibody (AB70122)

  • 4
  • 1
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MAATTANPEMTSDVPSLGPAIASGNSGPGIQGGGAIVQRAIKRRPGLDFDDDGEGNSKFLRCDDDQMSNDKERFARSDDEQSSADKERLARENHSEIERRRRNKMTAYITELSDMVPTCSALARKPDKLTILRMAVSHMKSLRGTGNTSTDGSYKPSFLTDQELKHLILEAADGFLFIVSCETGRVVYVSDSVTPVLNQPQSEWFGSTLYDQVHPDDVDKLREQLSTSENALTGRILDLKTGTVKKEGQQSSMRMCMGSRRSFICRMRCGSSSVDPVSVNRLSFVRNRCRNGLGSVKDGEPHFVVVHCTGYIKAWPPAGVSLPDDDPEAGQGSKFCLVAIGRLQVTSSPNCTDMSNVCQPTEFISRHNIEGIFTFVDHRCVATVGYQPQELLGKNIVEFCHPEDQQLLRDSFQQVVKLKGQVLSVMFRFRSKNQEWLWMRTSSFTFQNPYSDEIEYIICTNTNVKNSSQEPRPTLSNTIQRPQLGPTANLPLEMGSGQLAPRQQQQQTELDMVPGRDGLASYNHSQVVQPVTTTGPEHSKPLEKSDGLFAQDRDPRFSEIYHNINADQSKGISSSTVPATQQLFSQGNTFPPTPRPAENFRNSGLAPPVTIVQPSASAGQMLAQISRHSNPTQGATPTWTPTTRSGFSAQQVATQATAKTRTSQFGVGSFQTPSSFSSMSLPGAPTASPGAAAYPSLTNRGSNFAPETGQTAGQFQTRTAEGVGVWPQWQGQQPHHRSSSSEQHVQQPPAQQPGQPEVFQEMLSMLGDQSNSYNNEEFPDLTMFPPFSE","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":789,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P27540","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HIF1 beta also known as ARNT (Aryl Hydrocarbon Receptor Nuclear Translocator) plays an important role in cellular response to low oxygen levels. It partners with HIF1 alpha to form the hypoxia-inducible factor (HIF) complex. This beta subunit has a molecular weight of approximately 90 kDa and expresses ubiquitously in many cell types across different tissues. As part of the HIF complex HIF1 beta acts as a critical transcriptional regulator during hypoxic conditions.
Biological function summary

HIF1 beta regulates the expression of genes involved in adaptation to hypoxia. It functions as a dimerization partner in the HIF complex joining with HIF1 alpha or other proteins in the PAS superfamily to activate transcription of target genes. These genes are important for processes like angiogenesis metastasis and energy metabolism helping cells adapt to oxygen-deprived environments. The protein's interaction mainly influences cellular responses to hypoxia supporting tissue survival and function under stress.

Pathways

The involvement of HIF1 beta in the cellular hypoxia response is significant. It engages in the HIF pathway interacting closely with HIF1 alpha promoting the transcription of hypoxia-responsive genes when oxygen levels drop. This process integrates with larger pathways like the VEGF signaling pathway vital for blood vessel formation. Moreover its connection to ARNT serves motivation in the regulation of circadian rhythms showing the protein's broad relevance in cellular regulation.

HIF1 beta has associations with cancer and ischemic conditions. Aberrant activation of the HIF pathway where HIF1 beta is an important component can drive tumor progression through increased angiogenesis and metabolic reprogramming connecting it indirectly to VEGF. In ischemia altered gene expression mediated by HIF1 beta can influence tissue response to low oxygen contributing to disease pathology. Therefore targeting HIF1 beta might offer potential therapeutic avenues for these conditions.
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Specifications

Form

Liquid

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General info

Function

Required for activity of the AHR. Upon ligand binding, AHR translocates into the nucleus, where it heterodimerizes with ARNT and induces transcription by binding to xenobiotic response elements (XRE). Not required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding (PubMed : 34521881). The complex initiates transcription of genes involved in the regulation of a variety of biological processes, including angiogenesis, hematopoiesis, drug and lipid metabolism, cell motility and immune modulation (Probable). The heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters and functions as a transcriptional regulator of the adaptive response to hypoxia (By similarity). The heterodimer ARNT : AHR binds to core DNA sequence 5'-TGCGTG-3' within the dioxin response element (DRE) of target gene promoters and activates their transcription (PubMed : 28396409).

Subcellular localisation

Nucleus

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Product protocols

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Target data

Required for activity of the AHR. Upon ligand binding, AHR translocates into the nucleus, where it heterodimerizes with ARNT and induces transcription by binding to xenobiotic response elements (XRE). Not required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding (PubMed : 34521881). The complex initiates transcription of genes involved in the regulation of a variety of biological processes, including angiogenesis, hematopoiesis, drug and lipid metabolism, cell motility and immune modulation (Probable). The heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters and functions as a transcriptional regulator of the adaptive response to hypoxia (By similarity). The heterodimer ARNT : AHR binds to core DNA sequence 5'-TGCGTG-3' within the dioxin response element (DRE) of target gene promoters and activates their transcription (PubMed : 28396409).
See full target information ARNT
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