Recombinant Human HIF1 beta protein is a Human Full Length protein, in the 1 to 789 aa range, expressed in Wheat germ and suitable for ELISA, WB.
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Key facts
- Expression system
Wheat germ
- Tags
GST tag N-Terminus
- Applications
ELISA, WB
- Biologically active
No
Amino acid sequence
M A A T T A N P E M T S D V P S L G P A I A S G N S G P G I Q G G G A I V Q R A I K R R P G L D F D D D G E G N S K F L R C D D D Q M S N D K E R F A R S D D E Q S S A D K E R L A R E N H S E I E R R R R N K M T A Y I T E L S D M V P T C S A L A R K P D K L T I L R M A V S H M K S L R G T G N T S T D G S Y K P S F L T D Q E L K H L I L E A A D G F L F I V S C E T G R V V Y V S D S V T P V L N Q P Q S E W F G S T L Y D Q V H P D D V D K L R E Q L S T S E N A L T G R I L D L K T G T V K K E G Q Q S S M R M C M G S R R S F I C R M R C G S S S V D P V S V N R L S F V R N R C R N G L G S V K D G E P H F V V V H C T G Y I K A W P P A G V S L P D D D P E A G Q G S K F C L V A I G R L Q V T S S P N C T D M S N V C Q P T E F I S R H N I E G I F T F V D H R C V A T V G Y Q P Q E L L G K N I V E F C H P E D Q Q L L R D S F Q Q V V K L K G Q V L S V M F R F R S K N Q E W L W M R T S S F T F Q N P Y S D E I E Y I I C T N T N V K N S S Q E P R P T L S N T I Q R P Q L G P T A N L P L E M G S G Q L A P R Q Q Q Q Q T E L D M V P G R D G L A S Y N H S Q V V Q P V T T T G P E H S K P L E K S D G L F A Q D R D P R F S E I Y H N I N A D Q S K G I S S S T V P A T Q Q L F S Q G N T F P P T P R P A E N F R N S G L A P P V T I V Q P S A S A G Q M L A Q I S R H S N P T Q G A T P T W T P T T R S G F S A Q Q V A T Q A T A K T R T S Q F G V G S F Q T P S S F S S M S L P G A P T A S P G A A A Y P S L T N R G S N F A P E T G Q T A G Q F Q T R T A E G V G V W P Q W Q G Q Q P H H R S S S S E Q H V Q Q P P A Q Q P G Q P E V F Q E M L S M L G D Q S N S Y N N E E F P D L T M F P P F S E
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. The heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters and functions as a transcriptional regulator of the adaptive response to hypoxia (By similarity). The heterodimer ARNT:AHR binds to core DNA sequence 5'-TGCGTG-3' within the dioxin response element (DRE) of target gene promoters and activates their transcription (PubMed:28396409).
Alternative names
Aryl hydrocarbon receptor nuclear translocator, ARNT protein, Class E basic helix-loop-helix protein 2, Hypoxia-inducible factor 1-beta, bHLHe2, HIF-1-beta, HIF1-beta, BHLHE2, ARNT
Recommended products
Recombinant Human HIF1 beta protein is a Human Full Length protein, in the 1 to 789 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Alternative names
Aryl hydrocarbon receptor nuclear translocator, ARNT protein, Class E basic helix-loop-helix protein 2, Hypoxia-inducible factor 1-beta, bHLHe2, HIF-1-beta, HIF1-beta, BHLHE2, ARNT
Key facts
- Expression system
Wheat germ
- Tags
GST tag N-Terminus
- Applications
ELISA, WB
- Biologically active
No
- Accession
- P27540-1
- Animal free
No
- Species
Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
M A A T T A N P E M T S D V P S L G P A I A S G N S G P G I Q G G G A I V Q R A I K R R P G L D F D D D G E G N S K F L R C D D D Q M S N D K E R F A R S D D E Q S S A D K E R L A R E N H S E I E R R R R N K M T A Y I T E L S D M V P T C S A L A R K P D K L T I L R M A V S H M K S L R G T G N T S T D G S Y K P S F L T D Q E L K H L I L E A A D G F L F I V S C E T G R V V Y V S D S V T P V L N Q P Q S E W F G S T L Y D Q V H P D D V D K L R E Q L S T S E N A L T G R I L D L K T G T V K K E G Q Q S S M R M C M G S R R S F I C R M R C G S S S V D P V S V N R L S F V R N R C R N G L G S V K D G E P H F V V V H C T G Y I K A W P P A G V S L P D D D P E A G Q G S K F C L V A I G R L Q V T S S P N C T D M S N V C Q P T E F I S R H N I E G I F T F V D H R C V A T V G Y Q P Q E L L G K N I V E F C H P E D Q Q L L R D S F Q Q V V K L K G Q V L S V M F R F R S K N Q E W L W M R T S S F T F Q N P Y S D E I E Y I I C T N T N V K N S S Q E P R P T L S N T I Q R P Q L G P T A N L P L E M G S G Q L A P R Q Q Q Q Q T E L D M V P G R D G L A S Y N H S Q V V Q P V T T T G P E H S K P L E K S D G L F A Q D R D P R F S E I Y H N I N A D Q S K G I S S S T V P A T Q Q L F S Q G N T F P P T P R P A E N F R N S G L A P P V T I V Q P S A S A G Q M L A Q I S R H S N P T Q G A T P T W T P T T R S G F S A Q Q V A T Q A T A K T R T S Q F G V G S F Q T P S S F S S M S L P G A P T A S P G A A A Y P S L T N R G S N F A P E T G Q T A G Q F Q T R T A E G V G V W P Q W Q G Q Q P H H R S S S S E Q H V Q Q P P A Q Q P G Q P E V F Q E M L S M L G D Q S N S Y N N E E F P D L T M F P P F S E
- Accession
- Protein length
Full Length
- Amino acids
1 to 789
- Nature
Recombinant
- Tags
GST tag N-Terminus
Specifications
- Form
Liquid
General info
- Function
Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. The heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters and functions as a transcriptional regulator of the adaptive response to hypoxia (By similarity). The heterodimer ARNT:AHR binds to core DNA sequence 5'-TGCGTG-3' within the dioxin response element (DRE) of target gene promoters and activates their transcription (PubMed:28396409).
- Subcellular localisation
Nucleus
Storage
- Shipped at conditions
Dry Ice
- Appropriate long-term storage conditions
-80°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
U+00A0;
U+00A0;
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
HIF1 beta also known as ARNT (Aryl Hydrocarbon Receptor Nuclear Translocator) plays an important role in cellular response to low oxygen levels. It partners with HIF1 alpha to form the hypoxia-inducible factor (HIF) complex. This beta subunit has a molecular weight of approximately 90 kDa and expresses ubiquitously in many cell types across different tissues. As part of the HIF complex HIF1 beta acts as a critical transcriptional regulator during hypoxic conditions.
- Biological function summary
HIF1 beta regulates the expression of genes involved in adaptation to hypoxia. It functions as a dimerization partner in the HIF complex joining with HIF1 alpha or other proteins in the PAS superfamily to activate transcription of target genes. These genes are important for processes like angiogenesis metastasis and energy metabolism helping cells adapt to oxygen-deprived environments. The protein's interaction mainly influences cellular responses to hypoxia supporting tissue survival and function under stress.
- Pathways
The involvement of HIF1 beta in the cellular hypoxia response is significant. It engages in the HIF pathway interacting closely with HIF1 alpha promoting the transcription of hypoxia-responsive genes when oxygen levels drop. This process integrates with larger pathways like the VEGF signaling pathway vital for blood vessel formation. Moreover its connection to ARNT serves motivation in the regulation of circadian rhythms showing the protein's broad relevance in cellular regulation.
- Associated diseases and disorders
HIF1 beta has associations with cancer and ischemic conditions. Aberrant activation of the HIF pathway where HIF1 beta is an important component can drive tumor progression through increased angiogenesis and metabolic reprogramming connecting it indirectly to VEGF. In ischemia altered gene expression mediated by HIF1 beta can influence tissue response to low oxygen contributing to disease pathology. Therefore targeting HIF1 beta might offer potential therapeutic avenues for these conditions.
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1 product image
SDS-PAGE - Recombinant Human HIF1 beta protein (ab157907)
ab157907 on a 12.5% SDS-PAGE stained with Coomassie Blue.
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