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AB114964

Recombinant Human HIP1 protein

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Recombinant Human HIP1 protein is a Human Full Length protein, in the 1 to 1037 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

Huntingtin-interacting protein 1, HIP-1, Huntingtin-interacting protein I, HIP-I, HIP1

1 Images
SDS-PAGE - Recombinant Human HIP1 protein (AB114964)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human HIP1 protein (AB114964)

12.5% SDS-PAGE showing ab114964 at approximately 140.14kDa.
Stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

Tag free

Applications

WB, ELISA, SDS-PAGE

applications

Biologically active

No

Accession

O00291

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.3% Glutathione

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Recombinant protein.</p>" } } }
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Product details

This product was previously labelled as Huntingtin Interacting Protein HIP1.
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Sequence info

[{"sequence":"MDRMASSMKQVPNPLPKVLSRRGVGAGLEAAERESFERTQTVSINKAINTQEVAVKEKHARTCILGTHHEKGAQTFWSVVNRLPLSSNAVLCWKFCHVFHKLLRDGHPNVLKDSLRYRNELSDMSRMWGHLSEGYGQLCSIYLKLLRTKMEYHTKNPRFPGNLQMSDRQLDEAGESDVNNFFQLTVEMFDYLECELNLFQTVFNSLDMSRSVSVTAAGQCRLAPLIQVILDCSHLYDYTVKLLFKLHSCLPADTLQGHRDRFMEQFTKLKDLFYRSSNLQYFKRLIQIPQLPENPPNFLRASALSEHISPVVVIPAEASSPDSEPVLEKDDLMDMDASQQNLFDNKFDDIFGSSFSSDPFNFNSQNGVNKDEKDHLIERLYREISGLKAQLENMKTESQRVVLQLKGHVSELEADLAEQQHLRQQAADDCEFLRAELDELRRQREDTEKAQRSLSEIERKAQANEQRYSKLKEKYSELVQNHADLLRKNAEVTKQVSMARQAQVDLEREKKELEDSLERISDQGQRKTQEQLEVLESLKQELATSQRELQVLQGSLETSAQSEANWAAEFAELEKERDSLVSGAAHREEELSALRKELQDTQLKLASTEESMCQLAKDQRKMLLVGSRKAAEQVIQDALNQLEEPPLISCAGSADHLLSTVTSISSCIEQLEKSWSQYLACPEDISGLLHSITLLAHLTSDAIAHGATTCLRAPPEPADSLTEACKQYGRETLAYLASLEEEGSLENADSTAMRNCLSKIKAIGEELLPRGLDIKQEELGDLVDKEMAATSAAIETATARIEEMLSKSRAGDTGVKLEVNERILGCCTSLMQAIQVLIVASKDLQREIVESGRGTASPKEFYAKNSRWTEGLISASKAVGWGATVMVDAADLVVQGRGKFEELMVCSHEIAASTAQLVAASKVKADKDSPNLAQLQQASRGVNQATAGVVASTISGKSQIEETDNMDFSSMTLTQIKRQEMDSQVRVLELENELQKERQKLGELRKKHYELAGVAEGWEEGTEASPPTLQEVVTEKE","proteinLength":"Full Length","predictedMolecularWeight":"140.14 kDa","actualMolecularWeight":null,"aminoAcidEnd":1037,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"O00291","tags":[]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HIP1 also known as Huntingtin-interacting protein 1 is a protein with a mass of approximately 116 kDa. HIP1 is an adapter protein involved in the endocytosis process. It interacts with clathrin and AP2 adaptor complex assisting in the invagination and fission of clathrin-coated pits. HIP1 is present throughout various tissues including the brain and immune cells indicating its widespread role in cellular mechanisms.
Biological function summary

HIP1 plays an essential role in the modulation of receptor-mediated endocytosis. It is part of a larger complex with epsin and other proteins facilitating the internalization of extracellular signals. The protein's interaction with actin and lipid membranes suggests its function in reorganizing cytoskeleton arrangements vital for cell movement and shape changes. Cellular homeostasis and signal transduction rely on HIP1's ability to mediate these activities.

Pathways

HIP1 integrates into the PI3K/AKT signaling impacting cell survival and apoptosis. Its activity in clathrin-mediated endocytosis also links it to the receptor tyrosine kinase (RTK) pathway. In these pathways HIP1 assists in the regulation of growth factor receptors collaborating with proteins such as GRB2 and SHC1. These interactions ensure proper cellular responses to external stimuli.

HIP1 has associations with cancer and neurodegenerative diseases. In cancer overexpression of HIP1 enhances cell proliferation linked to its role in receptor trafficking and signaling. HIP1's interaction with huntingtin the protein mutated in Huntington's disease suggests its involvement in neurodegeneration. Such connections emphasize the protein's potential as a target for therapeutic intervention highlighting HIP1's impact on pathological conditions.
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Specifications

Form

Liquid

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General info

Function

Plays a role in clathrin-mediated endocytosis and trafficking (PubMed : 11532990, PubMed : 11577110, PubMed : 11889126). Involved in regulating AMPA receptor trafficking in the central nervous system in an NMDA-dependent manner (By similarity). Regulates presynaptic nerve terminal activity (By similarity). Enhances androgen receptor (AR)-mediated transcription (PubMed : 16027218). May act as a proapoptotic protein that induces cell death by acting through the intrinsic apoptosis pathway (PubMed : 11007801). Binds 3-phosphoinositides (via ENTH domain) (PubMed : 14732715). May act through the ENTH domain to promote cell survival by stabilizing receptor tyrosine kinases following ligand-induced endocytosis (PubMed : 14732715). May play a functional role in the cell filament networks (PubMed : 18790740). May be required for differentiation, proliferation, and/or survival of somatic and germline progenitors (PubMed : 11007801, PubMed : 12163454).

Sequence similarities

Belongs to the SLA2 family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Plays a role in clathrin-mediated endocytosis and trafficking (PubMed : 11532990, PubMed : 11577110, PubMed : 11889126). Involved in regulating AMPA receptor trafficking in the central nervous system in an NMDA-dependent manner (By similarity). Regulates presynaptic nerve terminal activity (By similarity). Enhances androgen receptor (AR)-mediated transcription (PubMed : 16027218). May act as a proapoptotic protein that induces cell death by acting through the intrinsic apoptosis pathway (PubMed : 11007801). Binds 3-phosphoinositides (via ENTH domain) (PubMed : 14732715). May act through the ENTH domain to promote cell survival by stabilizing receptor tyrosine kinases following ligand-induced endocytosis (PubMed : 14732715). May play a functional role in the cell filament networks (PubMed : 18790740). May be required for differentiation, proliferation, and/or survival of somatic and germline progenitors (PubMed : 11007801, PubMed : 12163454).
See full target information HIP1
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