Recombinant human HIPK1 protein
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Recombinant human HIPK1 protein is a Human Fragment protein, in the 156 to 555 aa range, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, FuncS.
View Alternative Names
KIAA0630, MYAK, NBAK2, HIPK1, Homeodomain-interacting protein kinase 1, Nuclear body-associated kinase 2
- FuncS
Unknown
Functional Studies - Recombinant human HIPK1 protein (AB60867)
Sample Kinase Activity Plot.
- FuncS
Unknown
Functional Studies - Recombinant human HIPK1 protein (AB60867)
Sample Kinase Activity Plot.
- FuncS
Unknown
Functional Studies - Recombinant human HIPK1 protein (AB60867)
The specific activity of HIPK1 (ab60867) was determined to be 79 nmol/min/mg as per activity assay protocol
- SDS-PAGE
Unknown
SDS-PAGE - Recombinant human HIPK1 protein (AB60867)
ab60867 on SDS-PAGE, MW ~71 kDa.
- SDS-PAGE
Unknown
SDS-PAGE - Recombinant human HIPK1 protein (AB60867)
SDS PAGE analysis of ab60867
Complementary Products
Reactivity data
Product details
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
HIPK1 participates in various cellular processes including apoptosis DNA damage response and Wnt signaling. It forms complexes with several transcription factors such as p53 and Smad proteins modifying their activity to influence cell fate. By interacting with transcription regulators HIPK1 modulates gene expression and has implications in cell growth and differentiation.
Pathways
HIPK1 interacts with proteins in important signaling cascades including the Wnt and JNK pathways. Notably HIPK1 phosphorylates components like c-Jun influencing the JNK pathway's role in stress response and apoptosis. Additionally in the Wnt pathway HIPK1 modulates β-catenin signaling which is important for regulating cell proliferation and differentiation. These interactions connect HIPK1 to wider cellular communications and responses.
Specifications
Form
Liquid
Additional notes
Affinity purified.
General info
Function
Serine/threonine-protein kinase involved in transcription regulation and TNF-mediated cellular apoptosis. Plays a role as a corepressor for homeodomain transcription factors. Phosphorylates DAXX and MYB. Phosphorylates DAXX in response to stress, and mediates its translocation from the nucleus to the cytoplasm. Inactivates MYB transcription factor activity by phosphorylation. Prevents MAP3K5-JNK activation in the absence of TNF. TNF triggers its translocation to the cytoplasm in response to stress stimuli, thus activating nuclear MAP3K5-JNK by derepression and promoting apoptosis. May be involved in anti-oxidative stress responses. Involved in the regulation of eye size, lens formation and retinal lamination during late embryogenesis. Promotes angiogenesis and to be involved in erythroid differentiation. May be involved in malignant squamous cell tumor formation. Phosphorylates PAGE4 at 'Thr-51' which is critical for the ability of PAGE4 to potentiate the transcriptional activator activity of JUN (PubMed : 24559171).
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. HIPK subfamily.
Post-translational modifications
Autophosphorylated. Phosphorylated and activated by JNK1.. Degraded by PARK7 at the protein level.. Sumoylated. When conjugated it is directed to nuclear speckles. SENP1-mediated desumoylation is mediated by TNF in response to stress stimuli, triggering transient translocation from nucleus to cytoplasm.
Target data
Product promise
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