Recombinant human HIPK4 protein is a Human Full Length protein, expressed in Baculovirus infected Sf9, with >70% purity and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >70% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
P u r i t y > 7 0 % b y d e n s i t o m e t r y .
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Protein kinase that phosphorylates human TP53 at Ser-9, and thus induces TP53 repression of BIRC5 promoter (By similarity). May act as a corepressor of transcription factors (Potential).
Alternative names
Homeodomain-interacting protein kinase 4, HIPK4
Recommended products
Recombinant human HIPK4 protein is a Human Full Length protein, expressed in Baculovirus infected Sf9, with >70% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >70% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Specific Activity: 202 nmol /min/mg as determined in kinase assay.
- Accession
- Q8NE63-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.0038% EGTA, 0.00292% EDTA, 0.00174% PMSF
Sequence info
Amino acid sequence
- P u r i t y > 7 0 % b y d e n s i t o m e t r y .
- Accession
- Q8NE63
- Protein length
- Full Length
- Nature
- Recombinant
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.Purity is lot specific. Please contact our technical Support team for details.
General info
- Function
Protein kinase that phosphorylates human TP53 at Ser-9, and thus induces TP53 repression of BIRC5 promoter (By similarity). May act as a corepressor of transcription factors (Potential).
- Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. HIPK subfamily.
- Post-translational modifications
Autophosphorylated.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
Native Pig Myelin Basic Protein ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
HIPK4 known as Homeodomain Interacting Protein Kinase 4 acts as a serine/threonine-protein kinase. It weighs around 69 kDa. This protein primarily localizes in the nucleus and occasionally the cytoplasm across various tissues. HIPK4 participates in phosphorylation processes modifying the activity of multiple substrates. Researchers often study it for its potential interactions with different transcription factors and regulators.
- Biological function summary
This protein plays an important role in regulating cell growth and differentiation. HIPK4 does not function as part of a complex but acts independently to impact these cellular processes. It can influence the expression of genes involved in apoptosis and cell cycle regulation indicating its significant influence on cellular homeostasis.
- Pathways
Researchers recognize HIPK4's involvement in the Wnt signaling and JNK pathways. The protein interacts with other kinases such as HIPK2 which also play roles in these pathways. These interactions suggest that HIPK4 might have diverse biological effects including those on development and cancer evolution.
- Associated diseases and disorders
Scientists have linked HIPK4 to cancer and neurodegenerative diseases. It interacts with proteins like p53 which are important in tumor suppression. HIPK4's alteration may contribute to the development of cancer by disturbing the balance of cell proliferation and death. Additionally its dysregulation could potentially connect to neuronal cell death implicating a role in neurodegenerative conditions.
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4 product images
Functional Studies - Recombinant human HIPK4 protein (ab69925)
The specific activity of HIPK4 (ab69925) was determined to be 175 nmol/min/mg as per activity assay protocol
SDS-PAGE - Recombinant human HIPK4 protein (ab69925)
SDS PAGE analysis of ab69925
Functional Studies - Recombinant human HIPK4 protein (ab69925)
SDS-PAGE - Recombinant human HIPK4 protein (ab69925)
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