Recombinant Human HIST1H2AK protein is a Human Fragment protein, in the 25 to 96 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Images
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
Amino acid sequence
Q F P V G R V H R L L R K G N Y A E R V G A G A P V Y L A A V L E Y L T A E I L E L A G N A A R D N K K T R I I P R H L Q L A I R N D E E L N K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Alternative names
H2AFP, HIST1H2AG, H2AC13, H2AFC, HIST1H2AI, H2AC15, H2AFD, HIST1H2AK, H2AC16, H2AFI, HIST1H2AL, H2AC17, H2AFN, HIST1H2AM, H2AC11, Histone H2A type 1, H2A.1, Histone H2A/ptl
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Recombinant Human HIST1H2AK protein is a Human Fragment protein, in the 25 to 96 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
- Accession
- P0C0S8-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- Q F P V G R V H R L L R K G N Y A E R V G A G A P V Y L A A V L E Y L T A E I L E L A G N A A R D N K K T R I I P R H L Q L A I R N D E E L N K
- Accession
- P0C0S8
- Protein length
- Fragment
- Amino acids
- 25 to 96
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
- Sequence similarities
Belongs to the histone H2A family.
- Post-translational modifications
Deiminated on Arg-4 in granulocytes upon calcium entry.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
HIST1H2AK also known as histone H2A type 1 or H2A.1 forms part of the histone protein family. This protein is a smaller component of chromatin with a molecular mass around 14 kDa. HIST1H2AK displays expression throughout various tissues where it contributes to the structural unit of nucleosomes. It participates in organizing DNA into chromatin being integral to compacting DNA within the nucleus and thereby regulating DNA accessibility.
- Biological function summary
Histone H2A type 1 plays an important role in DNA packaging and gene regulation. As part of the nucleosome it combines with other histone proteins to wrap DNA acting as the central unit of chromatin structure. It interacts with several nuclear proteins to modify chromatin’s biochemical state thereby affecting gene expression patterns. HIST1H2AK as a component of this histone complex participates in controlling genetic information critical for cellular function and response to environmental cues.
- Pathways
This histone protein type is linked to pathways managing chromatin remodeling and DNA repair processes. Its presence in the nucleosome assembly pathway associates it with epigenetic regulation mechanisms notably impacting gene silencing and expression adjustments through histone modifications. Related proteins such as histone H4 and histone deacetylases (HDACs) work together in these pathways to induce changes in chromatin conformation important for regulating access to genetic material.
- Associated diseases and disorders
The role of HIST1H2AK in chromatin structure implicates it in cancer and neurodegenerative diseases. Alterations in HIST1H2AK expression or modification often correlate with tumor progression and metastasis highlighting its involvement in oncogenic pathways. In neurodegenerative disorders interactions with proteins like p53 and HDACs suggest connections between irregular chromatin states and neuronal cell death indicating a potential involvement in diseases like Alzheimer's.
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1 product image
SDS-PAGE - Recombinant Human HIST1H2AK protein (ab159993)
ab159993 on a 12.5% SDS-PAGE stained with Coomassie Blue.
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