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Recombinant Human Histone H2B protein is a Human Full Length protein, in the 2 to 126 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.

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Images

SDS-PAGE - Recombinant Human Histone H2B protein (AB198637), expandable thumbnail

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

Biologically active

No

Amino acid sequence

M H H H H H H P E P A K S A P A P K K G S K K A V T K A Q K K D G K K R K R S R K E S Y S I Y V Y K V L K Q V H P D T G I S S K A M G I M N S F V N D I F E R I A G E A S R L A H Y N K R S T I T S R E I Q T A V R L L L P G E L A K H A V S E G T K A V T K Y T S S K

Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Associated Products

Select an associated product type

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Target data

Function

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.Has broad antibacterial activity. May contribute to the formation of the functional antimicrobial barrier of the colonic epithelium, and to the bactericidal activity of amniotic fluid.

Alternative names

Recommended products

Recombinant Human Histone H2B protein is a Human Full Length protein, in the 2 to 126 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Applications

SDS-PAGE

Accession
Q16778-1
Animal free

No

Species

Human

Concentration
Loading...
Storage buffer

pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.017% PMSF, 0.015% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

Sequence info

Amino acid sequence

M H H H H H H P E P A K S A P A P K K G S K K A V T K A Q K K D G K K R K R S R K E S Y S I Y V Y K V L K Q V H P D T G I S S K A M G I M N S F V N D I F E R I A G E A S R L A H Y N K R S T I T S R E I Q T A V R L L L P G E L A K H A V S E G T K A V T K Y T S S K

Accession

Q16778

Protein length

Full Length

Predicted molecular weight

15 kDa

Amino acids

2 to 126

Nature

Recombinant

Tags

His tag N-Terminus

Specifications

Form

Liquid

Additional notes

Affinity purified.

General info

Function

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Sequence similarities

Belongs to the histone H2B family.

Post-translational modifications

Monoubiquitination at Lys-35 (H2BK34Ub) by the MSL1/MSL2 dimer is required for histone H3 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) methylation and transcription activation at specific gene loci, such as HOXA9 and MEIS1 loci. Similarly, monoubiquitination at Lys-121 (H2BK120Ub) by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. H2BK120Ub also acts as a regulator of mRNA splicing: deubiquitination by USP49 is required for efficient cotranscriptional splicing of a large set of exons.

Subcellular localisation

Nucleus

Storage

Shipped at conditions

Dry Ice

Appropriate short-term storage conditions

-80°C

Appropriate long-term storage conditions

-80°C

Storage information

Avoid freeze / thaw cycle

Product promise

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1 product image

  • SDS-PAGE - Recombinant Human Histone H2B protein (ab198637), expandable thumbnail

    SDS-PAGE - Recombinant Human Histone H2B protein (ab198637)

    4-20% SDS-PAGE with Coomassie staining

    Lane 1: ab198637
    Lane 2: Protein marker

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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