Recombinant Human Histone H2B protein is a Human Full Length protein, expressed in Escherichia coli, with >=95% purity and suitable for WB, FuncS.
>=95% SDS-PAGE
Escherichia coli
Tag free
WB, FuncS
No
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application WB | Reactivity Reacts | Dilution info - | Notes ab92409 can be used as a WB positive control in conjunction with Anti-Histone H2B antibody - ChIP Grade ab1790. |
Application FuncS | Reactivity Reacts | Dilution info - | Notes Can be used as a substrate for in vitro enzymatic reactions, such as the acetylation of p300/CBP-Associated Factor (PCAF). |
Select an associated product type
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.Has broad antibacterial activity. May contribute to the formation of the functional antimicrobial barrier of the colonic epithelium, and to the bactericidal activity of amniotic fluid.
H2BFT, HIRIP1, HIST1H2BK, H2BC12, H2BFT, HIRIP1, HIST1H2BK, Histone H2B type 1-K, H2B K, HIRA-interacting protein 1
Recombinant Human Histone H2B protein is a Human Full Length protein, expressed in Escherichia coli, with >=95% purity and suitable for WB, FuncS.
>=95% SDS-PAGE
Escherichia coli
Tag free
WB, FuncS
No
No
Human
Liquid
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Belongs to the histone H2B family.
Monoubiquitination at Lys-35 (H2BK34Ub) by the MSL1/MSL2 dimer is required for histone H3 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) methylation and transcription activation at specific gene loci, such as HOXA9 and MEIS1 loci. Similarly, monoubiquitination at Lys-121 (H2BK120Ub) by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. H2BK120Ub also acts as a regulator of mRNA splicing: deubiquitination by USP49 is required for efficient cotranscriptional splicing of a large set of exons.
Nucleus
Dry Ice
-80°C
-80°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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Terms & Conditions.
All lanes: Western blot - Anti-Histone H2B antibody - ChIP Grade (Anti-Histone H2B antibody - ChIP Grade ab1790) at 0.1 µg/mL
All lanes: Western blot - Recombinant Human Histone H2B protein (ab92409) at 0.01 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 14 kDa
Exposure time: 2min
SDS-PAGE Analysis: Lane 1: MW Marker; Lane 2: 1.0 μg Human Histone H2B Protein (ab92409).
Western Blot Analysis. Lane 1: MW Marker; Lane 2: 100 ng Human Histone H2B Protein (ab92409) probed with Histone H2B pAb.
All lanes: Western blot - Recombinant Human Histone H2B protein (ab92409)
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