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Recombinant Human Histone H2B protein is a Human Full Length protein, expressed in Escherichia coli, with >=95% purity and suitable for WB, FuncS.

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Images

Western blot - Recombinant Human Histone H2B protein (AB92409), expandable thumbnail
  • SDS-PAGE - Recombinant Human Histone H2B protein (AB92409), expandable thumbnail
  • Western blot - Recombinant Human Histone H2B protein (AB92409), expandable thumbnail

Key facts

Purity

>=95% SDS-PAGE

Expression system

Escherichia coli

Tags

Tag free

Applications

WB, FuncS

Biologically active

No

Reactivity data

Application

WB

Reactivity

Reacts

Dilution info

-

Notes

ab92409 can be used as a WB positive control in conjunction with Anti-Histone H2B antibody - ChIP Grade ab1790.

Application

FuncS

Reactivity

Reacts

Dilution info

-

Notes

Can be used as a substrate for in vitro enzymatic reactions, such as the acetylation of p300/CBP-Associated Factor (PCAF).

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Target data

Function

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.Has broad antibacterial activity. May contribute to the formation of the functional antimicrobial barrier of the colonic epithelium, and to the bactericidal activity of amniotic fluid.

Alternative names

Recommended products

Recombinant Human Histone H2B protein is a Human Full Length protein, expressed in Escherichia coli, with >=95% purity and suitable for WB, FuncS.

Key facts

Purity

>=95% SDS-PAGE

Expression system

Escherichia coli

Applications

WB, FuncS

Accession
O60814-1
Animal free

No

Species

Human

Concentration
Loading...

Sequence info

Amino acid sequence

Accession

O60814

Protein length

Full Length

Nature

Recombinant

Specifications

Form

Liquid

General info

Function

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Sequence similarities

Belongs to the histone H2B family.

Post-translational modifications

Monoubiquitination at Lys-35 (H2BK34Ub) by the MSL1/MSL2 dimer is required for histone H3 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) methylation and transcription activation at specific gene loci, such as HOXA9 and MEIS1 loci. Similarly, monoubiquitination at Lys-121 (H2BK120Ub) by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. H2BK120Ub also acts as a regulator of mRNA splicing: deubiquitination by USP49 is required for efficient cotranscriptional splicing of a large set of exons.

Subcellular localisation

Nucleus

Storage

Shipped at conditions

Dry Ice

Appropriate short-term storage conditions

-80°C

Appropriate long-term storage conditions

-80°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Recombinant Human Histone H2B protein (ab92409), expandable thumbnail

    Western blot - Recombinant Human Histone H2B protein (ab92409)

    All lanes: Western blot - Anti-Histone H2B antibody - ChIP Grade (Anti-Histone H2B antibody - ChIP Grade ab1790) at 0.1 µg/mL

    All lanes: Western blot - Recombinant Human Histone H2B protein (ab92409) at 0.01 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 14 kDa

    Exposure time: 2min

  • SDS-PAGE - Recombinant Human Histone H2B protein (ab92409), expandable thumbnail

    SDS-PAGE - Recombinant Human Histone H2B protein (ab92409)

    SDS-PAGE Analysis: Lane 1: MW Marker; Lane 2: 1.0 μg Human Histone H2B Protein (ab92409).

  • Western blot - Recombinant Human Histone H2B protein (ab92409), expandable thumbnail

    Western blot - Recombinant Human Histone H2B protein (ab92409)

    Western Blot Analysis. Lane 1: MW Marker; Lane 2: 100 ng Human Histone H2B Protein (ab92409) probed with Histone H2B pAb.

    All lanes: Western blot - Recombinant Human Histone H2B protein (ab92409)

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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