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Recombinant human Histone H3 protein (Active) is a Human Full Length protein, in the 2 to 136 aa range, expressed in Escherichia coli, with >=90% purity and suitable for SDS-PAGE, FuncS.
Alternative names=Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3FD, H3C7, HIST1H3J, H3FJ, H3C12, HIST1H3I, H3FF, H3C11, HIST1H3H, H3FK, H3C10, HIST1H3G, H3FH, H3C8, HIST1H3F, H3FI, HIST1H3E, H3C6, HIST1H3D, H3FB, H3C4, HIST1H3C, H3FC, H3C3, HIST1H3B, H3FL, H3C2, HIST1H3A, H3FA, H3C1
- Purity
>=90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE, FuncS
- Biologically active
Yes
- Sequence
M M H H H H H H A R T K Q T A R K S T G G K A P R K Q L A T K A A R K S A P A T G G V K K P H R Y R P G T V A L R E I R R Y Q K S T E L L I R K L P F Q R L V R E I A Q D F K T D L R F Q S S A V M A L Q E A C E A Y L V G L F E D T N L C A I H A K R V T I M P K D I Q L A R R I R G E R A
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Images
Target data
Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
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Recombinant human Histone H3 protein (Active) is a Human Full Length protein, in the 2 to 136 aa range, expressed in Escherichia coli, with >=90% purity and suitable for SDS-PAGE, FuncS.
Alternative names=Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3FD, H3C7, HIST1H3J, H3FJ, H3C12, HIST1H3I, H3FF, H3C11, HIST1H3H, H3FK, H3C10, HIST1H3G, H3FH, H3C8, HIST1H3F, H3FI, HIST1H3E, H3C6, HIST1H3D, H3FB, H3C4, HIST1H3C, H3FC, H3C3, HIST1H3B, H3FL, H3C2, HIST1H3A, H3FA, H3C1
Key facts
- Purity
>=90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE, FuncS
- Biologically active
Yes
- Biological activity
Assay Conditions: ab198757 was coated onto a 96-well plate, and methylation by G9a was carried out at room temperature for 60 min. Methylated ab198757 was detected using a Chemiluminescence assay.
- Predicted molecular weight
15.4 kDa
- Amino acids
2 to 136
- Sequence
M M H H H H H H A R T K Q T A R K S T G G K A P R K Q L A T K A A R K S A P A T G G V K K P H R Y R P G T V A L R E I R R Y Q K S T E L L I R K L P F Q R L V R E I A Q D F K T D L R F Q S S A V M A L Q E A C E A Y L V G L F E D T N L C A I H A K R V T I M P K D I Q L A R R I R G E R A
- Accession
- P68431-1
- Protein length
Full Length
- Animal free
No
- Nature
Recombinant
- Species
Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: PBS, 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.0462% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.0165% Potassium chloride
Specifications
- Form
Liquid
- Additional notes
Affinity purified.
General info
- Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
- Sequence similarities
Belongs to the histone H3 family.
- Post-translational modifications
Acetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8me2s). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me). Acetylation at Lys-123 (H3K122ac) by EP300/p300 plays a central role in chromatin structure: localizes at the surface of the histone octamer and stimulates transcription, possibly by promoting nucleosome instability.
- Subcellular localisation
Nucleus
Storage
- Shipped at conditions
Dry Ice
- Appropriate long-term storage conditions
-80°C
- Storage information
Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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