Recombinant Human Histone H3.3 protein (denatured) is a Human Full Length protein, in the 1 to 136 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M A R T K Q T A R K S T G G K A P R K Q L A T K A A R K S A P S T G G V K K P H R Y R P G T V A L R E I R R Y Q K S T E L L I R K L P F Q R L V R E I A Q D F K T D L R F Q S A A I G A L Q E A S E A Y L V G L F E D T N L C A I H A K R V T I M P K D I Q L A R R I R G E R A
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Alternative names
Histone H3.3, H3F3B, H3-3A, H3.3A, H3F3, H3F3A, PP781, H3-3B, H3.3B
Recommended products
Recombinant Human Histone H3.3 protein (denatured) is a Human Full Length protein, in the 1 to 136 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Alternative names
Histone H3.3, H3F3B, H3-3A, H3.3A, H3F3, H3F3A, PP781, H3-3B, H3.3B
Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
- Accession
- P84243-1
- Animal free
No
- Species
Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris HCl
Sequence info
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M A R T K Q T A R K S T G G K A P R K Q L A T K A A R K S A P S T G G V K K P H R Y R P G T V A L R E I R R Y Q K S T E L L I R K L P F Q R L V R E I A Q D F K T D L R F Q S A A I G A L Q E A S E A Y L V G L F E D T N L C A I H A K R V T I M P K D I Q L A R R I R G E R A
- Accession
- Protein length
Full Length
- Predicted molecular weight
17.7 kDa
- Amino acids
1 to 136
- Nature
Recombinant
- Tags
His tag N-Terminus
Specifications
- Form
Liquid
General info
- Function
Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
- Sequence similarities
Belongs to the histone H3 family.
- Post-translational modifications
Acetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8me2s). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me). Acetylation at Lys-123 (H3K122ac) by EP300/p300 plays a central role in chromatin structure: localizes at the surface of the histone octamer and stimulates transcription, possibly by promoting nucleosome instability.
- Subcellular localisation
Nucleus
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Product promise
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1 product image
SDS-PAGE - Recombinant Human Histone H3.3 protein (denatured) (ab156709)
15% SDS-PAGE analysis of ab156709 (3μg).
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