Recombinant Human Histone H4 + Histone H3 protein Tetramer (His tag) is a Human Full Length protein, in the 1 to 103 aa range, expressed in Escherichia coli, with >=80% purity and suitable for SDS-PAGE.
Images
Key facts
- Purity
- >=80% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Sequence
This product is comprised of multiple sequences see
Amino acid sequence 1
M S G R G K G G K G L G K G G A K R H R K V L R D N I Q G I T K P A I R R L A R R G G V K R I S G L I Y E E T R G V L K V F L E N V I R D A V T Y T E H A K R K T V T A M D V V Y A L K R Q G R T L Y G F G G
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Additional Targets
H3C1
Alternative names
H4/A, H4FA, HIST1H4A, H4C2, H4/I, H4FI, HIST1H4B, H4C3, H4/G, H4FG, HIST1H4C, H4C4, H4/B, H4FB, HIST1H4D, H4C5, H4/J, H4FJ, HIST1H4E, H4C6, H4/C, H4FC, HIST1H4F, H4C8, H4/H, H4FH, HIST1H4H, H4C9, H4/M, H4FM, HIST1H4I, H4C11, H4/E, H4FE, HIST1H4J, H4C12, H4/D, H4FD, HIST1H4K, H4C13, H4/K, H4FK, HIST1H4L, H4C14, H4/N, H4F2, H4FN, HIST2H4, HIST2H4A, H4C15, H4/O, H4FO, HIST2H4B, H4C16, H4-16, HIST4H4, H4C1, Histone H4, H4K8cr
Recommended products
Recombinant Human Histone H4 + Histone H3 protein Tetramer (His tag) is a Human Full Length protein, in the 1 to 103 aa range, expressed in Escherichia coli, with >=80% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >=80% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P62805-1
- Animal free
- Yes
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 11.7% Sodium chloride, 10% Glycerol (glycerin, glycerine), 0.7% Tris HCl, 0.04% Ethylenediaminetetraacetic acid, disodium, dihydrate, 0.015% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence 1
- M S G R G K G G K G L G K G G A K R H R K V L R D N I Q G I T K P A I R R L A R R G G V K R I S G L I Y E E T R G V L K V F L E N V I R D A V T Y T E H A K R K T V T A M D V V Y A L K R Q G R T L Y G F G G
- Accession
- P62805
- Protein length
- Full Length
- Predicted molecular weight
- 57 kDa
- Amino acids
- 1 to 103
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Amino acid sequence 2
- M A R T K Q T A R K S T G G K A P R K Q L A T K A A R K S A P A T G G V K K P H R Y R P G T V A L R E I R R Y Q K S T E L L I R K L P F Q R L V R E I A Q D F K T D L R F Q S S A V M A L Q E A C E A Y L V G L F E D T N L C A I H A K R V T I M P K D I Q L A R R I R G E R A
- Accession
- P68431
- Protein length
- Full Length
- Predicted molecular weight
- 57 kDa
- Amino acids
- 1 to 136
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
- Sequence similarities
Belongs to the histone H4 family.
- Post-translational modifications
Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
Notes
Human recombinant histone tetramer, full length, consisting of 2 molecules each of histones H3 (a.a. 1-136(end)), and H4 (a.a. 1-103(end)), each with an N-terminal His-tag, expressed in an E. coli expression system.
Suitable as a substrate for Histone Methyltransferases.
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1 product image
Western blot - Recombinant Human Histone H4 + Histone H3 protein Tetramer (His tag) (ab278809)
SDS-PAGE analysis of ab278809 (2 μg).
4-20% SDS-PAGE. Coomassie staining.
All lanes: Western blot - Recombinant Human Histone H4 + Histone H3 protein Tetramer (His tag) (ab278809)
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