Recombinant Human Histone H4 protein is a Human Full Length protein, in the 2 to 103 aa range, expressed in Escherichia coli, with >=93% purity and suitable for SDS-PAGE.
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- EMBO reports 23:e542652022Reduced symmetric dimethylation stabilizes vimentin and promotes metastasis in MTAP-deficient lung cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWen-Hsin Chang,Yi-Ju Chen,Yi-Jing Hsiao,Ching-Cheng Chiang,Chia-Yu Wang,Ya-Ling Chang,Qi-Sheng Hong,Chien-Yu Lin,Shr-Uen Lin,Gee-Chen Chang,Hsuan-Yu Chen,Yu-Ju Chen,Ching-Hsien Chen,Pan-Chyr Yang,Sung-Liang YuPubMed 35766227
- Clinical epigenetics 12:1262020Circulating histone signature of human lean metabolic-associated fatty liver disease (MAFLD).Applications:Unspecified applicationReactive species:Unspecified reactive speciesDiana Buzova,Andrea Maugeri,Antonio Liguori,Cecilia Napodano,Oriana Lo Re,Jude Oben,Anna Alisi,Antonio Gasbarrini,Antonio Grieco,Jan Cerveny,Luca Miele,Manlio VinciguerraPubMed 32819448
Key facts
- Purity
- >=93% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
S G R G K G G K G L G K G G A K R H R K V L R D N I Q G I T K P A I R R L A R R G G V K R I S G L I Y E E T R G V L K V F L E N V I R D A V T Y T E H A K R K T V T A M D V V Y A L K R Q G R T L Y G F G G
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Alternative names
H4/A, H4FA, HIST1H4A, H4C2, H4/I, H4FI, HIST1H4B, H4C3, H4/G, H4FG, HIST1H4C, H4C4, H4/B, H4FB, HIST1H4D, H4C5, H4/J, H4FJ, HIST1H4E, H4C6, H4/C, H4FC, HIST1H4F, H4C8, H4/H, H4FH, HIST1H4H, H4C9, H4/M, H4FM, HIST1H4I, H4C11, H4/E, H4FE, HIST1H4J, H4C12, H4/D, H4FD, HIST1H4K, H4C13, H4/K, H4FK, HIST1H4L, H4C14, H4/N, H4F2, H4FN, HIST2H4, HIST2H4A, H4C15, H4/O, H4FO, HIST2H4B, H4C16, H4-16, HIST4H4, H4C1, Histone H4, H4K8cr
Recommended products
Recombinant Human Histone H4 protein is a Human Full Length protein, in the 2 to 103 aa range, expressed in Escherichia coli, with >=93% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >=93% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P62805-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% Potassium chloride
Sequence info
Amino acid sequence
- S G R G K G G K G L G K G G A K R H R K V L R D N I Q G I T K P A I R R L A R R G G V K R I S G L I Y E E T R G V L K V F L E N V I R D A V T Y T E H A K R K T V T A M D V V Y A L K R Q G R T L Y G F G G
- Accession
- P62805
- Protein length
- Full Length
- Predicted molecular weight
- 12.1 kDa
- Amino acids
- 2 to 103
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
- Sequence similarities
Belongs to the histone H4 family.
- Post-translational modifications
Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
Notes
ab198115 is useful as a substrate for histone methyltransferase and acetyltransferase assays. Ideal for screening small molecular inhibitors of histone modifying enzymes for drug discovery and HTS applications.
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1 product image
SDS-PAGE - Recombinant Human Histone H4 protein (ab198115)
4-20% SDS-PAGE analysis of ab198115.
Lane 1: 2 μg ab198115
Lane 2: Protein markerStained with Coomassie Blue
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