Recombinant Human HLA G protein (His tag) is a Human Full Length protein, in the 25 to 338 aa range, expressed in Mammalian, with >90% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Mammalian
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
D A A Q P A R R A V R S L H H H H H H H H H H L V P R G S R T G S H S M R Y F S A A V S R P G R G E P R F I A M G Y V D D T Q F V R F D S D S A C P R M E P R A P W V E Q E G P E Y W E E E T R N T K A H A Q T D R M N L Q T L R G Y Y N Q S E A S S H T L Q W M I G C D L G S D G R L L R G Y E Q Y A Y D G K D Y L A L N E D L R S W T A A D T A A Q I S K R K C E A A N V A E Q R R A Y L E G T C V E W L H R Y L E N G K E M L Q R A D P P K T H V T H H P V F D Y E A T L R C W A L G F Y P A E I I L T W Q R D G E D Q T Q D V E L V E T R P A G D G T F Q K W A A V V V P S G E E Q R Y T C H V Q H E G L P E P L M L R W K Q S S L P T I P I M G I V A G L V V L A A V V T G A A V A A V L W R K K S S D
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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3 products for Alternative Product
Target data
Function
Isoform 1. Non-classical major histocompatibility class Ib molecule involved in immune regulatory processes at the maternal-fetal interface (PubMed:19304799, PubMed:23184984, PubMed:29262349). In complex with B2M/beta-2 microglobulin binds a limited repertoire of nonamer self-peptides derived from intracellular proteins including histones and ribosomal proteins (PubMed:7584149, PubMed:8805247). Peptide-bound HLA-G-B2M complex acts as a ligand for inhibitory/activating KIR2DL4, LILRB1 and LILRB2 receptors on uterine immune cells to promote fetal development while maintaining maternal-fetal tolerance (PubMed:16366734, PubMed:19304799, PubMed:20448110, PubMed:23184984, PubMed:27859042, PubMed:29262349). Upon interaction with KIR2DL4 and LILRB1 receptors on decidual NK cells, it triggers NK cell senescence-associated secretory phenotype as a molecular switch to promote vascular remodeling and fetal growth in early pregnancy (PubMed:16366734, PubMed:19304799, PubMed:23184984, PubMed:29262349). Through interaction with KIR2DL4 receptor on decidual macrophages induces pro-inflammatory cytokine production mainly associated with tissue remodeling (PubMed:19304799). Through interaction with LILRB2 receptor triggers differentiation of type 1 regulatory T cells and myeloid-derived suppressor cells, both of which actively maintain maternal-fetal tolerance (PubMed:20448110, PubMed:27859042). May play a role in balancing tolerance and antiviral-immunity at maternal-fetal interface by keeping in check the effector functions of NK, CD8+ T cells and B cells (PubMed:10190900, PubMed:11290782, PubMed:24453251). Reprograms B cells toward an immune suppressive phenotype via LILRB1 (PubMed:24453251). May induce immune activation/suppression via intercellular membrane transfer (trogocytosis), likely enabling interaction with KIR2DL4, which resides mostly in endosomes (PubMed:20179272, PubMed:26460007). Through interaction with the inhibitory receptor CD160 on endothelial cells may control angiogenesis in immune privileged sites (PubMed:16809620). Isoform 2. Likely does not bind B2M and presents peptides. Negatively regulates NK cell- and CD8+ T cell-mediated cytotoxicity (PubMed:11290782). Isoform 3. Likely does not bind B2M and presents peptides. Negatively regulates NK cell- and CD8+ T cell-mediated cytotoxicity (PubMed:11290782). Isoform 4. Likely does not bind B2M and presents peptides. Negatively regulates NK cell- and CD8+ T cell-mediated cytotoxicity (PubMed:11290782). Isoform 5. Non-classical major histocompatibility class Ib molecule involved in immune regulatory processes at the maternal-fetal interface (PubMed:19304799, PubMed:23184984, PubMed:29262349). In complex with B2M/beta-2 microglobulin binds a limited repertoire of nonamer self-peptides derived from intracellular proteins including histones and ribosomal proteins (PubMed:7584149, PubMed:8805247). Peptide-bound HLA-G-B2M complex acts as a ligand for inhibitory/activating KIR2DL4, LILRB1 and LILRB2 receptors on uterine immune cells to promote fetal development while maintaining maternal-fetal tolerance (PubMed:16366734, PubMed:19304799, PubMed:20448110, PubMed:23184984, PubMed:29262349). Upon interaction with KIR2DL4 and LILRB1 receptors on decidual NK cells, it triggers NK cell senescence-associated secretory phenotype as a molecular switch to promote vascular remodeling and fetal growth in early pregnancy (PubMed:16366734, PubMed:19304799, PubMed:23184984, PubMed:29262349). Through interaction with KIR2DL4 receptor on decidual macrophages induces pro-inflammatory cytokine production mainly associated with tissue remodeling (PubMed:19304799). Through interaction with LILRB2 receptor triggers differentiation of type 1 regulatory T cells and myeloid-derived suppressor cells, both of which actively maintain maternal-fetal tolerance (PubMed:20448110). Reprograms B cells toward an immune suppressive phenotype via LILRB1 (PubMed:24453251). Isoform 6. Likely does not bind B2M and presents peptides. Isoform 7. Likely does not bind B2M and presents peptides.
Alternative names
HLA-6.0, HLAG, HLA-G, HLA G antigen, MHC class I antigen G
Recommended products
Recombinant Human HLA G protein (His tag) is a Human Full Length protein, in the 25 to 338 aa range, expressed in Mammalian, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Mammalian
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- P17693-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- D A A Q P A R R A V R S L H H H H H H H H H H L V P R G S R T G S H S M R Y F S A A V S R P G R G E P R F I A M G Y V D D T Q F V R F D S D S A C P R M E P R A P W V E Q E G P E Y W E E E T R N T K A H A Q T D R M N L Q T L R G Y Y N Q S E A S S H T L Q W M I G C D L G S D G R L L R G Y E Q Y A Y D G K D Y L A L N E D L R S W T A A D T A A Q I S K R K C E A A N V A E Q R R A Y L E G T C V E W L H R Y L E N G K E M L Q R A D P P K T H V T H H P V F D Y E A T L R C W A L G F Y P A E I I L T W Q R D G E D Q T Q D V E L V E T R P A G D G T F Q K W A A V V V P S G E E Q R Y T C H V Q H E G L P E P L M L R W K Q S S L P T I P I M G I V A G L V V L A A V V T G A A V A A V L W R K K S S D
- Accession
- P17693
- Protein length
- Full Length
- Predicted molecular weight
- 39.6 kDa
- Amino acids
- 25 to 338
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Isoform 1
- Sequence similarities
Belongs to the MHC class I family.
- Post-translational modifications
N-glycosylated.
- Subcellular localisation
- Early endosome membrane, Early endosome
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
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3 product images
SDS-PAGE - Recombinant Human HLA G protein (His tag) (ab225660)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis of ab225660 with 5% enrichment gel and 15% separation gel.
Mass Spectrometry - Recombinant Human HLA G protein (His tag) (ab225660)
Based on the SEQUEST from database of Mammalian Cell host and target protein, the LC-MS/MS Analysis result of ab225660 could indicate that this peptide derived from Mammalian Cell-expressed human HLA-G.
Mass Spectrometry - Recombinant Human HLA G protein (His tag) (ab225660)
Based on the SEQUEST from database of Mammalian Cell host and target protein, the LC-MS/MS Analysis result of ab225660 could indicate that this peptide derived from Mammalian Cell-expressed human HLA-G.
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