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AB123176

Recombinant Human HMBS/PBGD protein

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Recombinant Human HMBS/PBGD protein is a Human Full Length protein, in the 1 to 361 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

PBGD, UPS, HMBS, Porphobilinogen deaminase, PBG-D, Hydroxymethylbilane synthase, Pre-uroporphyrinogen synthase

1 Images
SDS-PAGE - Recombinant Human HMBS/PBGD protein (AB123176)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human HMBS/PBGD protein (AB123176)

15% SDS-PAGE analysis of ab123176 (3ug)

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

P08397

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as HMBS
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSHMSGNGNAAATAEENSPKMRVIRVGTRKSQLARIQTDSVVATLKASYPGLQFEIIAMSTTGDKILDTALSKIGEKSLFTKELEHALEKNEVDLVVHSLKDLPTVLPPGFTIGAICKRENPHDAVVFHPKFVGKTLETLPEKSVVGTSSLRRAAQLQRKFPHLEFRSIRGNLNTRLRKLDEQQEFSAIILATAGLQRMGWHNRVGQILHPEECMYAVGQGALGVEVRAKDQDILDLVGVLHDPETLLRCIAERAFLRHLEGGCSVPVAVHTAMKDGQLYLTGGVWSLDGSDSIQETMQATIHVPAQHEDGPEDDPQLVGITARNIPRGPQLAAQNLGISLANLLLSKGAKNILDVARQLNDAH","proteinLength":"Full Length","predictedMolecularWeight":"41.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":361,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P08397","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Specifications

Form

Liquid

Additional notes

ab123176 is purified using conventional chromatography techniques.

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General info

Function

As part of the heme biosynthetic pathway, catalyzes the sequential polymerization of four molecules of porphobilinogen to form hydroxymethylbilane, also known as preuroporphyrinogen (PubMed : 18004775, PubMed : 18936296, PubMed : 19138865, PubMed : 23815679). Catalysis begins with the assembly of the dipyrromethane cofactor by the apoenzyme from two molecules of porphobilinogen or from preuroporphyrinogen. The covalently linked cofactor acts as a primer, around which the tetrapyrrole product is assembled. In the last step of catalysis, the product, preuroporphyrinogen, is released, leaving the cofactor bound to the holodeaminase intact (PubMed : 18936296).

Sequence similarities

Belongs to the HMBS family.

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Product protocols

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Target data

As part of the heme biosynthetic pathway, catalyzes the sequential polymerization of four molecules of porphobilinogen to form hydroxymethylbilane, also known as preuroporphyrinogen (PubMed : 18004775, PubMed : 18936296, PubMed : 19138865, PubMed : 23815679). Catalysis begins with the assembly of the dipyrromethane cofactor by the apoenzyme from two molecules of porphobilinogen or from preuroporphyrinogen. The covalently linked cofactor acts as a primer, around which the tetrapyrrole product is assembled. In the last step of catalysis, the product, preuroporphyrinogen, is released, leaving the cofactor bound to the holodeaminase intact (PubMed : 18936296).
See full target information HMBS
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Complementary Products

Primary Antibodies

AB129092

Anti-HMBS/PBGD antibody [EPR8105]

RabMAb|Recombinant

Western blot - Anti-HMBS/PBGD antibody [EPR8105] (AB129092)

  • 0
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Primary Antibodies

AB248171

Anti-UBE4B antibody [EPR7471] - BSA and Azide free

RabMAb|Recombinant

Western blot - Anti-UBE4B antibody [EPR7471] - BSA and Azide free (AB248171)

  • 0
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Primary Antibodies

AB169766

Anti-CPOX antibody [EPR11927(B)]

RabMAb|Recombinant

Immunoprecipitation - Anti-CPOX antibody [EPR11927(B)] (AB169766)

  • 0
  • 0
Primary Antibodies

AB75768

Anti-SMC1A (phospho S957) antibody [EPR2857Y]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC1A (phospho S957) antibody [EPR2857Y] (AB75768)

  • 0
  • 0

Product promise

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