Recombinant Human HMG4 protein is a Human Fragment protein, in the 1 to 180 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M A K G D P K K P K G K M S A Y A F F V Q T C R E E H K K K N P E V P V N F A E F S K K C S E R W K T M S G K E K S K F D E M A K A D K V R Y D R E M K D Y G P A K G G K K K K D P N A P K R P P S G F F L F C S E F R P K I K S T N P G I S I G D V A K K L G E M W N N L N D S E K Q P Y I T K A A K L K E K Y E K D V A D Y K S K G K F D G A K G P A K V A R K K V
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Multifunctional protein with various roles in different cellular compartments. May act in a redox sensitive manner. Associates with chromatin and binds DNA with a preference for non-canonical DNA structures such as single-stranded DNA. Can bend DNA and enhance DNA flexibility by looping thus providing a mechanism to promote activities on various gene promoters (By similarity). Proposed to be involved in the innate immune response to nucleic acids by acting as a cytoplasmic promiscuous immunogenic DNA/RNA sensor (By similarity). Negatively regulates B-cell and myeloid cell differentiation. In hematopoietic stem cells may regulate the balance between self-renewal and differentiation. Involved in negative regulation of canonical Wnt signaling (By similarity).
Alternative names
HMG2A, HMG4, HMGB3, High mobility group protein B3, High mobility group protein 2a, High mobility group protein 4, HMG-2a, HMG-4
Recommended products
Recombinant Human HMG4 protein is a Human Fragment protein, in the 1 to 180 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- O15347-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M A K G D P K K P K G K M S A Y A F F V Q T C R E E H K K K N P E V P V N F A E F S K K C S E R W K T M S G K E K S K F D E M A K A D K V R Y D R E M K D Y G P A K G G K K K K D P N A P K R P P S G F F L F C S E F R P K I K S T N P G I S I G D V A K K L G E M W N N L N D S E K Q P Y I T K A A K L K E K Y E K D V A D Y K S K G K F D G A K G P A K V A R K K V
- Accession
- O15347
- Protein length
- Fragment
- Predicted molecular weight
- 22.8 kDa
- Amino acids
- 1 to 180
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab167851 was purified using conventional chromatography techniques.
General info
- Function
Multifunctional protein with various roles in different cellular compartments. May act in a redox sensitive manner. Associates with chromatin and binds DNA with a preference for non-canonical DNA structures such as single-stranded DNA. Can bend DNA and enhance DNA flexibility by looping thus providing a mechanism to promote activities on various gene promoters (By similarity). Proposed to be involved in the innate immune response to nucleic acids by acting as a cytoplasmic promiscuous immunogenic DNA/RNA sensor (By similarity). Negatively regulates B-cell and myeloid cell differentiation. In hematopoietic stem cells may regulate the balance between self-renewal and differentiation. Involved in negative regulation of canonical Wnt signaling (By similarity).
- Sequence similarities
Belongs to the HMGB family.
- Post-translational modifications
Reduction/oxidation of cysteine residues Cys-23, Cys-45 and Cys-104 and a possible intramolecular disulfide bond involving Cys-23 and Cys-45 give rise to different redox forms with specific functional activities in various cellular compartments: 1- fully reduced HMGB3 (HMGB3C23hC45hC104h), 2- disulfide HMGB3 (HMGB3C23-C45C104h) and 3- sulfonyl HMGB3 (HMGB3C23soC45soC104so).
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
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1 product image
SDS-PAGE - Recombinant Human HMG4 protein (ab167851)
15% SDS-PAGE analysis of 3µg ab167851.
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