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Recombinant Human HMGE protein is a Human Full Length protein, in the 28 to 217 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.

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Images

SDS-PAGE - Recombinant Human HMGE protein (AB101203), expandable thumbnail

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, MS

Biologically active

No

Amino acid sequence

M G S S H H H H H H S S G L V P R G S H M C T A T K Q K N S G Q N L E E D M G Q S E Q K A D P P A T E K T L L E E K V K L E E Q L K E T V E K Y K R A L A D T E N L R Q R S Q K L V E E A K L Y G I Q A F C K D L L E V A D V L E K A T Q C V P K E E I K D D N P H L K N L Y E G L V M T E V Q I Q K V F T K H G L L K L N P V G A K F D P Y E H E A L F H T P V E G K E P G T V A L V S K V G Y K L H G R T L R P A L V G V V K E A

Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Application

MS

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner (By similarity). Seems to control the nucleotide-dependent binding of mitochondrial HSP70 to substrate proteins (PubMed:11311562).

Alternative names

Recommended products

Recombinant Human HMGE protein is a Human Full Length protein, in the 28 to 217 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Applications

SDS-PAGE, MS

Accession
Q9HAV7-1
Animal free

No

Species

Human

Concentration
Loading...
Storage buffer

pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 1.16% Sodium chloride, 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

Sequence info

Amino acid sequence

M G S S H H H H H H S S G L V P R G S H M C T A T K Q K N S G Q N L E E D M G Q S E Q K A D P P A T E K T L L E E K V K L E E Q L K E T V E K Y K R A L A D T E N L R Q R S Q K L V E E A K L Y G I Q A F C K D L L E V A D V L E K A T Q C V P K E E I K D D N P H L K N L Y E G L V M T E V Q I Q K V F T K H G L L K L N P V G A K F D P Y E H E A L F H T P V E G K E P G T V A L V S K V G Y K L H G R T L R P A L V G V V K E A

Accession

Q9HAV7

Protein length

Full Length

Predicted molecular weight

23.6 kDa

Amino acids

28 to 217

Nature

Recombinant

Tags

His tag N-Terminus

Specifications

Form

Liquid

Additional notes

ab101203 is purified using conventional chromatography techniques.

General info

Function

Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner (By similarity). Seems to control the nucleotide-dependent binding of mitochondrial HSP70 to substrate proteins (PubMed:11311562).

Sequence similarities

Belongs to the GrpE family.

Subcellular localisation

Mitochondrion matrix

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Previously labelled as GRPEL1.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

HMGE also known as High Mobility Group E functions as a dynamic chromatin-associated protein facilitating structural changes in chromatin composition. This protein weighs approximately 25 kDa. HMGE is highly expressed in the nucleus of most eukaryotic cells where it modulates DNA accessibility and influences transcriptional activity. Its presence in various tissues indicates its ubiquitous role in gene regulation processes.

Biological function summary

HMGE plays a role in shifting chromatin for the transcriptional machinery. It acts as a non-histone chromatin factor and it may participate in specific multiprotein complexes that aid in nucleosome positioning. This protein affects gene expression patterns by altering the chromatin state therefore significantly impacting cellular responses to extracellular signals. HMGE's chromatin remodeling is important for regulating genes involved in growth and differentiation.

Pathways

HMGE is instrumental in the chromatin remodeling and DNA repair pathways. It interacts with acetylation enzymes to influence gene transcription activities related to cellular growth. HMGE shows interactions with histone variants and modification proteins facilitating these epigenetic modifications. Furthermore it participates in DNA repair pathways working in conjunction with repair proteins such as PARP1.

Associated diseases and disorders

HMGE has been associated with cancer and neurological disorders. Aberrant expression or mutations in HMGE can contribute to oncogenesis as it may influence the expression of oncogenes and tumor suppressor genes. In neurological disorders its role in chromatin dynamics and gene regulation can affect neuronal gene expression possibly relating to conditions like Alzheimer's disease. In these contexts HMGE might interact with p53 protein in cancer and APP protein in the neurological domain illustrating its importance in pathological states.

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1 product image

  • SDS-PAGE - Recombinant Human HMGE protein (ab101203), expandable thumbnail

    SDS-PAGE - Recombinant Human HMGE protein (ab101203)

    15% SDS-PAGE showing ab101203 at approximately 23.6kDa (3µg).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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