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AB229173

Recombinant human HMT protein (Active)

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Recombinant human HMT protein (Active) is a Human Full Length protein, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, FuncS, Mass Spec.

View Alternative Names

Histamine N-methyltransferase, HMT, HNMT

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SDS-PAGE - Recombinant human HMT protein (Active) (AB229173)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human HMT protein (Active) (AB229173)

15% SDS-PAGE analysis of 3 μg ab229173.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

Specific activity is > 200 nmol/min/mg, and is defined as the amount of enzyme that transfers 1.0 nmole of methyl group per minute at 37°C.

Accession

P50135

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.32% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Previously labelled as HNMT.
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Sequence info

[{"sequence":"MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSMASSMRSLFSDHGKYVESFRRFLNHSTEHQCMQEFMDKKLPGIIGRIGDTKSEIKILSIGGGAGEIDLQILSKVQAQYPGVCINNEVVEPSAEQIAKYKELVAKTSNLENVKFAWHKETSSEYQSRMLEKKELQKWDFIHMIQMLYYVKDIPATLKFFHSLLGTNAKMLIIVVSGSSGWDKLWKKYGSRFPQDDLCQYITSDDLTQMLDNLGLKYECYDLLSTMDISDCFIDGDENGDLLWDFLTETCNFNATAPPDLRAELGKDLQEPEFSAKKEGKVLFNNTLSFIVIEA","proteinLength":"Full Length","predictedMolecularWeight":"37.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P50135","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HMT also known as HMT-H8 or Histone Methyltransferase 8 is an enzyme involved in the transfer of methyl groups to histone proteins an essential step in chromatin modification. It has a molecular mass of approximately 55 kDa. HMT expresses in various tissues with a notable presence in the liver brain and heart tissues. The enzyme's distribution suggests a widespread role in regulating gene expression across different biological systems.
Biological function summary

HMT functions within the context of chromatin remodeling influencing how tightly DNA coiled around histones is therefore impacting gene accessibility. HMT often partners with other proteins to form complexes that precisely regulate methylation status in active transcription sites. By adding methyl groups HMT plays a role in activating or repressing genes affecting a broad range of cellular processes.

Pathways

The enzyme HMT takes part in significant epigenetic pathways such as the histone modification pathway and the gene silencing pathway. It actively interacts with proteins like HDACs (histone deacetylases) within these pathways affecting deacetylation and methylation in chromatin dynamics. HMT's activity often intersects with proteins like DNMTs (DNA methyltransferases) which collaborate to maintain cellular identity through stable gene expression patterns.

Abnormal changes in HMT's activity have been linked to cancer and neurodegenerative diseases. Elevated HMT activity often correlates with certain cancer types where increased methylation leads to the silencing of tumor suppressor genes. In neurodegenerative contexts HMT dysfunction can affect neuronal gene expression contributing to conditions like Huntington's disease. Proteins like p53 and HDAC are often implicated in these disorders alongside HMT as their interactions shift cellular behavior in disease states.
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Specifications

Form

Liquid

Additional notes

ab229173 was purified using conventional chromatography.

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General info

Function

Inactivates histamine by N-methylation. Plays an important role in degrading histamine and in regulating the airway response to histamine.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. HNMT family.

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Product protocols

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Target data

Inactivates histamine by N-methylation. Plays an important role in degrading histamine and in regulating the airway response to histamine.
See full target information Histamine N-methyltransferase
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