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Recombinant Human HNF-4-alpha protein is a Human Full Length protein, in the 1 to 464 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.

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Expression system

Wheat germ

Tags

Tag free

Applications

SDS-PAGE, ELISA, WB

Biologically active

No

Sequence

M R L S K T L V D M D M A D Y S A A L D P A Y T T L E F E N V Q V L T M G N D T S P S E G T N L N A P N S L G V S A L C A I C G D R A T G K H Y G A S S C D G C K G F F R R S V R K N H M Y S C R F S R Q C V V D K D K R N Q C R Y C R L K K C F R A G M K K E A V Q N E R D R I S T R R S S Y E D S S L P S I N A L L Q A E V L S R Q I T S P V S G I N G D I R A K K I A S I A D V C E S M K E Q L L V L V E W A K Y I P A F C E L P L D D Q V A L L R A H A G E H L L L G A T K R S M V F K D V L L L G N D Y I V P R H C P E L A E M S R V S I R I L D E L V L P F Q E L Q I D D N E Y A Y L K A I I F F D P D A K G L S D P G K I K R L R S Q V Q V S L E D Y I N D R Q Y D S R G R F G E L L L L L P T L Q S I T W Q M I E Q I Q F I K L F G M A K I D N L L Q E M L L G G S P S D A P H A H H P L H P H L M Q E H M G T N V I V A N T M P T H L S N G Q M S T P E T P Q P S P P G G S G S E P Y K L L P G A V A T I V K P L S A I P Q P T I T K Q E V I

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Shipping To:
United States

Reactivity data

Application
SDS-PAGE
Reactivity
Reacts
Dilution info
-
Notes

-

Application
ELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Application
WB
Reactivity
Reacts
Dilution info
-
Notes
(Recombinant protein).

Images

Target data

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Recombinant Human HNF-4-alpha protein is a Human Full Length protein, in the 1 to 464 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.

Key facts

Expression system

Wheat germ

Applications

SDS-PAGE, ELISA, WB

Predicted molecular weight

78 kDa

Amino acids

1 to 464

Sequence

M R L S K T L V D M D M A D Y S A A L D P A Y T T L E F E N V Q V L T M G N D T S P S E G T N L N A P N S L G V S A L C A I C G D R A T G K H Y G A S S C D G C K G F F R R S V R K N H M Y S C R F S R Q C V V D K D K R N Q C R Y C R L K K C F R A G M K K E A V Q N E R D R I S T R R S S Y E D S S L P S I N A L L Q A E V L S R Q I T S P V S G I N G D I R A K K I A S I A D V C E S M K E Q L L V L V E W A K Y I P A F C E L P L D D Q V A L L R A H A G E H L L L G A T K R S M V F K D V L L L G N D Y I V P R H C P E L A E M S R V S I R I L D E L V L P F Q E L Q I D D N E Y A Y L K A I I F F D P D A K G L S D P G K I K R L R S Q V Q V S L E D Y I N D R Q Y D S R G R F G E L L L L L P T L Q S I T W Q M I E Q I Q F I K L F G M A K I D N L L Q E M L L G G S P S D A P H A H H P L H P H L M Q E H M G T N V I V A N T M P T H L S N G Q M S T P E T P Q P S P P G G S G S E P Y K L L P G A V A T I V K P L S A I P Q P T I T K Q E V I

Accession
P41235-2
Protein length

Full Length

Animal free

No

Nature

Recombinant

Species

Human

Concentration
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Storage buffer

pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione

Specifications

Form

Liquid

General info

Function

Transcriptional regulator which controls the expression of hepatic genes during the transition of endodermal cells to hepatic progenitor cells, facilitating the recruitment of RNA pol II to the promoters of target genes (PubMed:30597922). Activates the transcription of CYP2C38 (By similarity). Represses the CLOCK-ARNTL/BMAL1 transcriptional activity and is essential for circadian rhythm maintenance and period regulation in the liver and colon cells (PubMed:30530698).

Sequence similarities

Belongs to the nuclear hormone receptor family. NR2 subfamily.

Post-translational modifications

Phosphorylated on tyrosine residue(s); phosphorylation is important for its DNA-binding activity. Phosphorylation may directly or indirectly play a regulatory role in the subnuclear distribution. Phosphorylation at Ser-313 by AMPK reduces the ability to form homodimers and bind DNA.

Subcellular localisation

Nucleus

Storage

Shipped at conditions

Dry Ice

Appropriate long-term storage conditions

-80°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Product promise

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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