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AB123212

Recombinant Human hnRNP A1 protein (His tag N-Terminus)

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(7 Publications)

Recombinant Human hnRNP A1 protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 320 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

View Alternative Names

HNRPA1, HNRNPA1, Heterogeneous nuclear ribonucleoprotein A1, hnRNP A1, Helix-destabilizing protein, Single-strand RNA-binding protein, hnRNP core protein A1

1 Images
SDS-PAGE - Recombinant Human hnRNP A1 protein (His tag N-Terminus) (AB123212)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human hnRNP A1 protein (His tag N-Terminus) (AB123212)

15% SDS-PAGE analysis of ab123212 (3 μg)

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P09651

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 40% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMSKSESPKEPEQLRKLFIGGLSFETTDESLRSHFEQWGTLTDCVVMRDPNTKRSRGFGFVTYATVEEVDAAMNARPHKVDGRVVEPKRAVSREDSQRPGAHLTVKKIFVGGIKEDTEEHHLRDYFEQYGKIEVIEIMTDRGSGKKRGFAFVTFDDHDSVDKIVIQKYHTVNGHNCEVRKALSKQEMASASSSQRGRSGSGNFGGGRGGGFGGNDNFGRGGNFSGRGGFGGSRGGGGYGGSGDGYNGFGNDGSNFGGGGSYNDFGNYNNQSSNFGPMKGGNFGGRSSGPYGGGGQYFAKPRNQGGYGGSSSSSSYGSGRRF","proteinLength":"Full Length","predictedMolecularWeight":"36.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":320,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P09651","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) also known just as hnRNP A1 is a multifunctional protein that plays important roles in RNA processing mechanisms. It is a member of the hnRNP protein family involved in the packaging of nascent pre-mRNA. hnRNP A1 has a molecular mass of approximately 34-38 kDa and shows a widespread expression in various cells and tissues. This protein is recognized for its ability to bind to RNA molecules contributing to their proper processing splicing and transport within the cellular context.
Biological function summary

HnRNP A1 is involved in RNA binding and splicing contributing to the formation of spliceosomes the complexes responsible for pre-mRNA splicing. The protein aids in alternative splicing influencing mRNA diversity and stability. hnRNP A1 interacts with other proteins in the hnRNP family remodeling ribonucleoprotein complexes and affecting their functions. These interactions ensure proper mRNA maturation impacting gene expression regulation within the cell.

Pathways

HnRNP A1 is integral to critical cellular mechanisms such as the regulation of mRNA transport and splicing. It influences the DNA damage response pathway by modulating gene expression required for cellular repair and stability. hnRNP A1 also cooperates with other RNA binding proteins like the splicing factors SR proteins to orchestrate the splicing machinery. These interactions are pivotal in maintaining cellular homeostasis and response to stress.

HnRNP A1 is notably related to neurodegenerative diseases like Amyotrophic Lateral Sclerosis (ALS) and certain types of cancer. Abnormalities in its expression or mutations can lead to disrupted cellular processes contributing to disease pathology. In ALS dysregulation of hnRNP A1 affects neuronal RNA metabolism and is associated with the TDP-43 protein a marker of neurodegeneration. In cancer hnRNP A1 influences tumor development through its role in alternative splicing and gene expression modification linking it to oncogenic signaling pathways.
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Specifications

Form

Liquid

Additional notes

ab123212 was purified using conventional chromatography and is > 90% pure by SDS - PAGE.

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General info

Function

Involved in the packaging of pre-mRNA into hnRNP particles, transport of poly(A) mRNA from the nucleus to the cytoplasm and modulation of splice site selection (PubMed : 17371836). Plays a role in the splicing of pyruvate kinase PKM by binding repressively to sequences flanking PKM exon 9, inhibiting exon 9 inclusion and resulting in exon 10 inclusion and production of the PKM M2 isoform (PubMed : 20010808). Binds to the IRES and thereby inhibits the translation of the apoptosis protease activating factor APAF1 (PubMed : 31498791). May bind to specific miRNA hairpins (PubMed : 28431233).. (Microbial infection) May play a role in HCV RNA replication.. (Microbial infection) Cleavage by Enterovirus 71 protease 3C results in increased translation of apoptosis protease activating factor APAF1, leading to apoptosis.

Post-translational modifications

Arg-194, Arg-206 and Arg-225 are dimethylated, probably to asymmetric dimethylarginine.. Sumoylated.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Involved in the packaging of pre-mRNA into hnRNP particles, transport of poly(A) mRNA from the nucleus to the cytoplasm and modulation of splice site selection (PubMed : 17371836). Plays a role in the splicing of pyruvate kinase PKM by binding repressively to sequences flanking PKM exon 9, inhibiting exon 9 inclusion and resulting in exon 10 inclusion and production of the PKM M2 isoform (PubMed : 20010808). Binds to the IRES and thereby inhibits the translation of the apoptosis protease activating factor APAF1 (PubMed : 31498791). May bind to specific miRNA hairpins (PubMed : 28431233).. (Microbial infection) May play a role in HCV RNA replication.. (Microbial infection) Cleavage by Enterovirus 71 protease 3C results in increased translation of apoptosis protease activating factor APAF1, leading to apoptosis.
See full target information HNRNPA1
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in genetics 13:969895 PubMed36338958

2022

Candidate genes and sequence variants for susceptibility to mycobacterial infection identified by whole-exome sequencing.

Applications

Unspecified application

Species

Unspecified reactive species

Alexander Varzari,Igor V Deyneko,Gitte Hoffmann Bruun,Maja Dembic,Winfried Hofmann,Victor M Cebotari,Sergei S Ginda,Brage S Andresen,Thomas Illig

Nucleic acid therapeutics 32:378-390 PubMed35833796

2022

Antisense Oligonucleotide Rescue of Deep-Intronic Variants Activating Pseudoexons in the 6-Pyruvoyl-Tetrahydropterin Synthase Gene.

Applications

Unspecified application

Species

Unspecified reactive species

Ainhoa Martínez-Pizarro,Fátima Leal,Lise Lolle Holm,Thomas K Doktor,Ulrika S S Petersen,María Bueno,Beat Thöny,Belén Pérez,Brage S Andresen,Lourdes R Desviat

Human mutation 43:253-265 PubMed34923709

2021

Vulnerable exons, like ACADM exon 5, are highly dependent on maintaining a correct balance between splicing enhancers and silencers.

Applications

Unspecified application

Species

Unspecified reactive species

Lise L Holm,Thomas K Doktor,Michael B Hansen,Ulrika S S Petersen,Brage S Andresen

Molecular oncology 16:594-606 PubMed34418284

2021

hnRNP-A1 binds to the IRES of MELOE-1 antigen to promote MELOE-1 translation in stressed melanoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Maud Charpentier,Emilie Dupré,Agnès Fortun,Floriane Briand,Mike Maillasson,Emmanuelle Com,Charles Pineau,Nathalie Labarrière,Catherine Rabu,François Lang

Developmental cell 56:478-493.e11 PubMed33476555

2021

Genome-wide analysis of pseudogenes reveals HBBP1's human-specific essentiality in erythropoiesis and implication in β-thalassemia.

Applications

Unspecified application

Species

Unspecified reactive species

Yanni Ma,Siqi Liu,Jie Gao,Chunyan Chen,Xin Zhang,Hao Yuan,Zhongyang Chen,Xiaolin Yin,Chenguang Sun,Yanan Mao,Fanqi Zhou,Yi Shao,Qian Liu,Jiayue Xu,Li Cheng,Daqi Yu,Pingping Li,Ping Yi,Jiahuan He,Guangfeng Geng,Qing Guo,Yanmin Si,Hualu Zhao,Haipeng Li,Graham L Banes,He Liu,Yukio Nakamura,Ryo Kurita,Yue Huang,Xiaoshuang Wang,Fang Wang,Gang Fang,James Douglas Engel,Lihong Shi,Yong E Zhang,Jia Yu

Scientific reports 10:22155 PubMed33335114

2020

Identification of hnRNP-A1 as a pharmacodynamic biomarker of type I PRMT inhibition in blood and tumor tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Paul B Noto,Timothy W Sikorski,Francesca Zappacosta,Craig D Wagner,Rocio Montes de Oca,Matthew E Szapacs,Roland S Annan,Yan Liu,Charles F McHugh,Helai P Mohammad,Steven P Piccoli,Caretha L Creasy

Proceedings of the National Academy of Sciences of 115:E4604-E4612 PubMed29712837

2018

Mechanistic studies of a small-molecule modulator of SMN2 splicing.

Applications

Unspecified application

Species

Unspecified reactive species

Jingxin Wang,Peter G Schultz,Kristen A Johnson
View all publications
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