Recombinant Human hnRNP A2B1 protein (Tagged) is a Human Full Length protein, in the 1 to 249 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M E R E K E Q F R K L F I G G L S F E T T E E S L R N Y Y E Q W G K L T D C V V M R D P A S K R S R G F G F V T F S S M A E V D A A M A A R P H S I D G R V V E P K R A V A R E E S G K P G A H V T V K K L F V G G I K E D T E E H H L R D Y F E E Y G K I D T I E I I T D R Q S G K K R G F G F V T F D D H D P V D K I V L Q K Y H T I N G H N A E V R K A L S R Q E M Q E D L E V A I L E V A P V M E E E E E D M V V E D L D M A T R V G A T E V V M T T M E E E I M E V E I T M I L E I I T S N L L T T V Q
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Heterogeneous nuclear ribonucleoprotein (hnRNP) that associates with nascent pre-mRNAs, packaging them into hnRNP particles. The hnRNP particle arrangement on nascent hnRNA is non-random and sequence-dependent and serves to condense and stabilize the transcripts and minimize tangling and knotting. Packaging plays a role in various processes such as transcription, pre-mRNA processing, RNA nuclear export, subcellular location, mRNA translation and stability of mature mRNAs (PubMed:19099192). Forms hnRNP particles with at least 20 other different hnRNP and heterogeneous nuclear RNA in the nucleus. Involved in transport of specific mRNAs to the cytoplasm in oligodendrocytes and neurons: acts by specifically recognizing and binding the A2RE (21 nucleotide hnRNP A2 response element) or the A2RE11 (derivative 11 nucleotide oligonucleotide) sequence motifs present on some mRNAs, and promotes their transport to the cytoplasm (PubMed:10567417). Specifically binds single-stranded telomeric DNA sequences, protecting telomeric DNA repeat against endonuclease digestion (By similarity). Also binds other RNA molecules, such as primary miRNA (pri-miRNAs): acts as a nuclear 'reader' of the N6-methyladenosine (m6A) mark by specifically recognizing and binding a subset of nuclear m6A-containing pri-miRNAs. Binding to m6A-containing pri-miRNAs promotes pri-miRNA processing by enhancing binding of DGCR8 to pri-miRNA transcripts (PubMed:26321680). Involved in miRNA sorting into exosomes following sumoylation, possibly by binding (m6A)-containing pre-miRNAs (PubMed:24356509). Acts as a regulator of efficiency of mRNA splicing, possibly by binding to m6A-containing pre-mRNAs (PubMed:26321680). Plays a role in the splicing of pyruvate kinase PKM by binding repressively to sequences flanking PKM exon 9, inhibiting exon 9 inclusion and resulting in exon 10 inclusion and production of the PKM M2 isoform (PubMed:20010808). Also plays a role in the activation of the innate immune response (PubMed:31320558). Mechanistically, senses the presence of viral DNA in the nucleus, homodimerizes and is demethylated by JMJD6 (PubMed:31320558). In turn, translocates to the cytoplasm where it activates the TBK1-IRF3 pathway, leading to interferon alpha/beta production (PubMed:31320558). (Microbial infection) Involved in the transport of HIV-1 genomic RNA out of the nucleus, to the microtubule organizing center (MTOC), and then from the MTOC to the cytoplasm: acts by specifically recognizing and binding the A2RE (21 nucleotide hnRNP A2 response element) sequence motifs present on HIV-1 genomic RNA, and promotes its transport.
Alternative names
HNRPA2B1, HNRNPA2B1, Heterogeneous nuclear ribonucleoproteins A2/B1, hnRNP A2/B1
Recommended products
Recombinant Human hnRNP A2B1 protein (Tagged) is a Human Full Length protein, in the 1 to 249 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P22626-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- M E R E K E Q F R K L F I G G L S F E T T E E S L R N Y Y E Q W G K L T D C V V M R D P A S K R S R G F G F V T F S S M A E V D A A M A A R P H S I D G R V V E P K R A V A R E E S G K P G A H V T V K K L F V G G I K E D T E E H H L R D Y F E E Y G K I D T I E I I T D R Q S G K K R G F G F V T F D D H D P V D K I V L Q K Y H T I N G H N A E V R K A L S R Q E M Q E D L E V A I L E V A P V M E E E E E D M V V E D L D M A T R V G A T E V V M T T M E E E I M E V E I T M I L E I I T S N L L T T V Q
- Accession
- P22626
- Protein length
- Full Length
- Predicted molecular weight
- 42.4 kDa
- Amino acids
- 1 to 249
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Heterogeneous nuclear ribonucleoprotein (hnRNP) that associates with nascent pre-mRNAs, packaging them into hnRNP particles. The hnRNP particle arrangement on nascent hnRNA is non-random and sequence-dependent and serves to condense and stabilize the transcripts and minimize tangling and knotting. Packaging plays a role in various processes such as transcription, pre-mRNA processing, RNA nuclear export, subcellular location, mRNA translation and stability of mature mRNAs (PubMed:19099192). Forms hnRNP particles with at least 20 other different hnRNP and heterogeneous nuclear RNA in the nucleus. Involved in transport of specific mRNAs to the cytoplasm in oligodendrocytes and neurons: acts by specifically recognizing and binding the A2RE (21 nucleotide hnRNP A2 response element) or the A2RE11 (derivative 11 nucleotide oligonucleotide) sequence motifs present on some mRNAs, and promotes their transport to the cytoplasm (PubMed:10567417). Specifically binds single-stranded telomeric DNA sequences, protecting telomeric DNA repeat against endonuclease digestion (By similarity). Also binds other RNA molecules, such as primary miRNA (pri-miRNAs): acts as a nuclear 'reader' of the N6-methyladenosine (m6A) mark by specifically recognizing and binding a subset of nuclear m6A-containing pri-miRNAs. Binding to m6A-containing pri-miRNAs promotes pri-miRNA processing by enhancing binding of DGCR8 to pri-miRNA transcripts (PubMed:26321680). Involved in miRNA sorting into exosomes following sumoylation, possibly by binding (m6A)-containing pre-miRNAs (PubMed:24356509). Acts as a regulator of efficiency of mRNA splicing, possibly by binding to m6A-containing pre-mRNAs (PubMed:26321680). Plays a role in the splicing of pyruvate kinase PKM by binding repressively to sequences flanking PKM exon 9, inhibiting exon 9 inclusion and resulting in exon 10 inclusion and production of the PKM M2 isoform (PubMed:20010808). Also plays a role in the activation of the innate immune response (PubMed:31320558). Mechanistically, senses the presence of viral DNA in the nucleus, homodimerizes and is demethylated by JMJD6 (PubMed:31320558). In turn, translocates to the cytoplasm where it activates the TBK1-IRF3 pathway, leading to interferon alpha/beta production (PubMed:31320558).
- Post-translational modifications
Sumoylated in exosomes, promoting miRNAs-binding.
- Subcellular localisation
- Nucleus, Nucleoplasm
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
HnRNP A2B1 sometimes referred to as heterogeneous nuclear ribonucleoprotein A2/B1 is a multifunctional protein involved in RNA binding and processing. It has a molecular mass of about 38-43 kDa. The protein is expressed in various tissues with high expression levels observed in the brain and liver. hnRNP A2B1 participates in a range of RNA-related processes playing a significant role in mRNA metabolism and transport.
- Biological function summary
HnRNP A2B1 contributes to alternative splicing RNA stability and translational regulation. This protein typically associates with complex assemblies that manage RNA processing and transport mechanisms. It interacts with other proteins within the heterogeneous nuclear ribonucleoprotein family collaborating in the regulation of mRNA cycling between the nucleus and cytoplasm affecting gene expression patterns.
- Pathways
HnRNP A2B1 exhibits critical involvement in the mRNA splicing pathway and RNA transport pathways. It frequently interacts with proteins such as hnRNP A1 and hnRNP C where they collectively influence pre-mRNA processing and splicing dictating proper RNA maturation and cell function. These pathways are important for maintaining cellular homeostasis and responding to cellular signals.
- Associated diseases and disorders
HnRNP A2B1 is linked to neurodegenerative diseases and certain cancers. Abnormal expression or mutations of hnRNP A2B1 are associated with Alzheimer's disease implicating the protein in amyloid precursor protein processing. Additionally dysregulation of this protein shows correlation with breast cancer progression. Proteins like tau and other hnRNPs connect with hnRNP A2B1 in these conditions suggesting a significant role in pathological states.
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SDS-PAGE - Recombinant Human hnRNP A2B1 protein (Tagged) (ab235707)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis with 5% enrichment gel and 15% separation gel of ab235707.
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