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AB114680

Recombinant Human hnRNP F protein

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Recombinant Human hnRNP F protein is a Human Full Length protein, in the 1 to 415 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

HNRPF, HNRNPF, Heterogeneous nuclear ribonucleoprotein F, hnRNP F, Nucleolin-like protein mcs94-1

1 Images
SDS-PAGE - Recombinant Human hnRNP F protein (AB114680)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human hnRNP F protein (AB114680)

ab114680 analysed on a 12.5% SDS-PAGE gel stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

Tag free

Applications

WB, SDS-PAGE, ELISA

applications

Biologically active

No

Accession

P52597

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.3% Glutathione

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MMLGPEGGEGFVVKLRGLPWSCSVEDVQNFLSDCTIHDGAAGVHFIYTREGRQSGEAFVELGSEDDVKMALKKDRESMGHRYIEVFKSHRTEMDWVLKHSGPNSADSANDGFVRLRGLPFGCTKEEIVQFFSGLEIVPNGITLPVDPEGKITGEAFVQFASQELAEKALGKHKERIGHRYIEVFKSSQEEVRSYSDPPLKFMSVQRPGPYDRPGTARRYIGIVKQAGLERMRPGAYSTGYGGYEEYSGLSDGYGFTTDLFGRDLSYCLSGMYDHRYGDSEFTVQSTTGHCVHMRGLPYKATENDIYNFFSPLNPVRVHIEIGPDGRVTGEADVEFATHEEAVAAMSKDRANMQHRYIELFLNSTTGASNGAYSSQVMQGMGVSAAQATYSGLESQSVSGCYGAGYSGQNSMGGYD","proteinLength":"Full Length","predictedMolecularWeight":"71.72 kDa","actualMolecularWeight":null,"aminoAcidEnd":415,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P52597","tags":[]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Heterogeneous nuclear ribonucleoprotein F (hnRNP F) also known as hnRNP-F or HNRNPF plays an important role in RNA processing within the nucleus. It belongs to the family of hnRNPs which bind to nascent pre-mRNA molecules influencing their stability and transport. HnRNP F has a molecular weight of approximately 46 kDa. It is ubiquitously expressed found in various tissues but it exhibits higher levels of expression in the testis and brain. Its structured domains allow it to bind to RNA with high specificity impacting several RNA metabolic processes directly.
Biological function summary

HnRNP F interacts with RNA molecules to modulate alternative splicing mRNA stability and transport. It often forms part of larger ribonucleoprotein complexes that assemble on pre-mRNA. These complexes facilitate the accurate processing of pre-mRNA by guiding the necessary splicing machinery. HnRNP F commonly associates with other proteins such as hnRNP H enhancing its ability to regulate splice site selection and influencing mRNA diversity and function within the cell.

Pathways

HnRNP F is importantly involved in the regulation of gene expression through the mRNA splicing pathway. It particularly affects the spliceosome assembly a multi-protein complex essential for the removal of introns. hnRNP F's involvement in this pathway intersects with proteins such as SF2/ASF and U2AF65 which are key regulators in splicing. Furthermore hnRNP F influences the response to cellular stress pathways by stabilizing specific mRNAs under stress conditions often cooperating with proteins involved in stress granules.

HnRNP F has significant associations with neurodegenerative diseases and cancer. In neurodegenerative diseases aberrant splicing events influenced by hnRNP F are linked to conditions such as Alzheimer's disease where it interacts with splicing factors like SRSF1. In cancer altered expression or malfunction of hnRNP F contributes to splicing variants that promote oncogenesis notably in association with proteins like MYC which drives cancer cell proliferation. These connections make hnRNP F a potential target for therapeutic interventions in disease treatment.
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Specifications

Form

Liquid

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General info

Function

Component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Plays a role in the regulation of alternative splicing events. Binds G-rich sequences in pre-mRNAs and keeps target RNA in an unfolded state.

Post-translational modifications

Sumoylated.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Plays a role in the regulation of alternative splicing events. Binds G-rich sequences in pre-mRNAs and keeps target RNA in an unfolded state.
See full target information HNRNPF
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Complementary Products

Primary Antibodies

AB289974

Anti-hnRNP H antibody [EPR25302-12]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP H antibody [EPR25302-12] (AB289974)

  • 0
  • 0
Primary Antibodies

AB177152

Anti-hnRNP A1 antibody [EPR12768]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-hnRNP A1 antibody [EPR12768] (AB177152)

  • 0
  • 0
Primary Antibodies

AB205531

Anti-GRSF1 antibody [EPR16678]

RabMAb|Recombinant

Western blot - Anti-GRSF1 antibody [EPR16678] (AB205531)

  • 0
  • 0
Primary Antibodies

AB184946

Anti-hnRNP Q antibody [EPR15096] - C-terminal

RabMAb|Recombinant

Western blot - Anti-hnRNP Q antibody [EPR15096] - C-terminal (AB184946)

  • 5
  • 1

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