Recombinant Human hnRNP K protein is a Human Full Length protein, in the 1 to 463 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
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- Neuroscience bulletin 39:947-9612023CircFhit Modulates GABAergic Synaptic Transmission via Regulating the Parental Gene Fhit Expression in the Spinal Dorsal Horn in a Rat Model of Neuropathic Pain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTing Xu,Zhen-Yu Li,Meng Liu,Su-Bo Zhang,Huan-Huan Ding,Jia-Yan Wu,Su-Yan Lin,Jun Liu,Jia-You Wei,Xue-Qin Zhang,Wen-Jun XinPubMed 36637791
- Nature communications 13:48652022HnRNPK maintains single strand RNA through controlling double-strand RNA in mammalian cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSagar Mahale,Meenakshi Setia,Bharat Prajapati,Santhilal Subhash,Mukesh Pratap Yadav,Subazini Thankaswamy Kosalai,Ananya Deshpande,Jagannath Kuchlyan,Mirco Di Marco,Fredrik Westerlund,L Marcus Wilhelmsson,Chandrasekhar Kanduri,Meena KanduriPubMed 36038571
- Nature communications 10:20712019The transcription factor CBFB suppresses breast cancer through orchestrating translation and transcription.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNavdeep Malik et. al.PubMed 31061501
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
M E T E Q P E E T F P N T E T N G E F G K R P A E D M E E E Q A F K R S R N T D E M V E L R I L L Q S K N A G A V I G K G G K N I K A L R T D Y N A S V S V P D S S G P E R I L S I S A D I E T I G E I L K K I I P T L E E G L Q L P S P T A T S Q L P L E S D A V E C L N Y Q H Y K G S D F D C E L R L L I H Q S L A G G I I G V K G A K I K E L R E N T Q T T I K L F Q E C C P H S T D R V V L I G G K P D R V V E C I K I I L D L I S E S P I K G R A Q P Y D P N F Y D E T Y D Y G G F T M M F D D R R G R P V G F P M R G R G G F D R M P P G R G G R P M P P S R R D Y D D M S P R R G P P P P P P G R G G R G G S R A R N L P L P P P P P P R G G D L M A Y D R R G R P G D R Y D G M V G F S A D E T W D S A I D T W S P S E W Q M A Y E P Q G G S G Y D Y S Y A G G R G S Y G D L G G P I I T T Q V T I P K D L A G S I I G K G G Q R I K Q I R H E S G A S I K I D E P L E G S E D R I I T I T G T Q D Q I Q N A Q Y L L Q N S V K Q Y S G K F F
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA. Plays an important role in p53/TP53 response to DNA damage, acting at the level of both transcription activation and repression. When sumoylated, acts as a transcriptional coactivator of p53/TP53, playing a role in p21/CDKN1A and 14-3-3 sigma/SFN induction (By similarity). As far as transcription repression is concerned, acts by interacting with long intergenic RNA p21 (lincRNA-p21), a non-coding RNA induced by p53/TP53. This interaction is necessary for the induction of apoptosis, but not cell cycle arrest. As part of a ribonucleoprotein complex composed at least of ZNF827, HNRNPL and the circular RNA circZNF827 that nucleates the complex on chromatin, may negatively regulate the transcription of genes involved in neuronal differentiation (PubMed:33174841).
Alternative names
HNRPK, HNRNPK, Heterogeneous nuclear ribonucleoprotein K, hnRNP K, Transformation up-regulated nuclear protein, TUNP
Recommended products
Recombinant Human hnRNP K protein is a Human Full Length protein, in the 1 to 463 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- P61978-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M E T E Q P E E T F P N T E T N G E F G K R P A E D M E E E Q A F K R S R N T D E M V E L R I L L Q S K N A G A V I G K G G K N I K A L R T D Y N A S V S V P D S S G P E R I L S I S A D I E T I G E I L K K I I P T L E E G L Q L P S P T A T S Q L P L E S D A V E C L N Y Q H Y K G S D F D C E L R L L I H Q S L A G G I I G V K G A K I K E L R E N T Q T T I K L F Q E C C P H S T D R V V L I G G K P D R V V E C I K I I L D L I S E S P I K G R A Q P Y D P N F Y D E T Y D Y G G F T M M F D D R R G R P V G F P M R G R G G F D R M P P G R G G R P M P P S R R D Y D D M S P R R G P P P P P P G R G G R G G S R A R N L P L P P P P P P R G G D L M A Y D R R G R P G D R Y D G M V G F S A D E T W D S A I D T W S P S E W Q M A Y E P Q G G S G Y D Y S Y A G G R G S Y G D L G G P I I T T Q V T I P K D L A G S I I G K G G Q R I K Q I R H E S G A S I K I D E P L E G S E D R I I T I T G T Q D Q I Q N A Q Y L L Q N S V K Q Y S G K F F
- Accession
- P61978
- Protein length
- Full Length
- Predicted molecular weight
- 77.4 kDa
- Amino acids
- 1 to 463
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA. Plays an important role in p53/TP53 response to DNA damage, acting at the level of both transcription activation and repression. When sumoylated, acts as a transcriptional coactivator of p53/TP53, playing a role in p21/CDKN1A and 14-3-3 sigma/SFN induction (By similarity). As far as transcription repression is concerned, acts by interacting with long intergenic RNA p21 (lincRNA-p21), a non-coding RNA induced by p53/TP53. This interaction is necessary for the induction of apoptosis, but not cell cycle arrest. As part of a ribonucleoprotein complex composed at least of ZNF827, HNRNPL and the circular RNA circZNF827 that nucleates the complex on chromatin, may negatively regulate the transcription of genes involved in neuronal differentiation (PubMed:33174841).
- Post-translational modifications
Arg-296 and Arg-299 are dimethylated, probably to asymmetric dimethylarginine.
- Subcellular localisation
- Nucleus, Nucleoplasm
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Heterogeneous nuclear ribonucleoprotein K (hnRNP K) is a multifunctional protein that plays a critical role in RNA metabolism including RNA splicing translation and transcription. Alternate names for hnRNP K include synkaryon-associated protein and hnRNP KurB. It has a molecular mass of approximately 51 kDa and is ubiquitously expressed in the nucleus and cytoplasm of eukaryotic cells. This protein features three KH (K homology) domains that enable it to interact with nucleic acids and other proteins.
- Biological function summary
HnRNP K serves as a transcriptional regulator and participates in the assembly of ribonucleoprotein complexes. As part of the ribonucleoprotein complex it interacts with both RNA and DNA influencing various gene expression pathways. It also modulates the activity of several transcription factors including c-Myc and has been implicated in cell proliferation and differentiation. This protein's versatile functions make it an important component in various cellular processes.
- Pathways
HnRNP K plays an important role in signal transduction and the p53 pathway. In the p53 pathway hnRNP K acts as a coactivator enhancing the transcriptional activity of the tumor suppressor p53 and influencing cell cycle regulation and apoptosis. Additionally hnRNP K interacts with several proteins including the c-Myc oncogene to regulate gene expression associated with growth and cell cycle progression.
- Associated diseases and disorders
HnRNP K has been linked to cancer and neurological disorders. Its interaction with c-Myc makes it relevant in various cancers where c-Myc is dysregulated contributing to unchecked cell proliferation. Furthermore alterations in hnRNP K expression or function have been associated with neurodegenerative disorders where it might interact with proteins like TDP-43 or FUS affecting neuronal function and survival.
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1 product image
SDS-PAGE - Recombinant Human hnRNP K protein (ab132460)
SDS-PAGE analysis of ab132460 on a 12.5% gel stained with Coomassie Blue.
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