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AB132460

Recombinant Human hnRNP K protein (GST tag N-Terminus)

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(3 Publications)

Recombinant Human hnRNP K protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 463 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

HNRPK, HNRNPK, Heterogeneous nuclear ribonucleoprotein K, hnRNP K, Transformation up-regulated nuclear protein, TUNP

1 Images
SDS-PAGE - Recombinant Human hnRNP K protein (GST tag N-Terminus) (AB132460)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human hnRNP K protein (GST tag N-Terminus) (AB132460)

SDS-PAGE analysis of ab132460 on a 12.5% gel stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

ELISA, SDS-PAGE, WB

applications

Biologically active

No

Accession

P61978

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Complementary Products

Primary Antibodies

AB52600

Anti-hnRNP K antibody [EP943Y]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP K antibody [EP943Y] (AB52600)

  • 4
  • 2
Primary Antibodies

AB181171

Anti-HNRPH2/HNRNPH2 antibody [EPR12171]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNRPH2/HNRNPH2 antibody [EPR12171] (AB181171)

  • 5
  • 2
Primary Antibodies

AB133607

Anti-hnRNP C1/C2 antibody [EPNCIR152]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP C1/C2 antibody [EPNCIR152] (AB133607)

  • 5
  • 2
Proteins & Peptides

AB114680

Recombinant Human hnRNP F protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human hnRNP F protein (AB114680)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"METEQPEETFPNTETNGEFGKRPAEDMEEEQAFKRSRNTDEMVELRILLQSKNAGAVIGKGGKNIKALRTDYNASVSVPDSSGPERILSISADIETIGEILKKIIPTLEEGLQLPSPTATSQLPLESDAVECLNYQHYKGSDFDCELRLLIHQSLAGGIIGVKGAKIKELRENTQTTIKLFQECCPHSTDRVVLIGGKPDRVVECIKIILDLISESPIKGRAQPYDPNFYDETYDYGGFTMMFDDRRGRPVGFPMRGRGGFDRMPPGRGGRPMPPSRRDYDDMSPRRGPPPPPPGRGGRGGSRARNLPLPPPPPPRGGDLMAYDRRGRPGDRYDGMVGFSADETWDSAIDTWSPSEWQMAYEPQGGSGYDYSYAGGRGSYGDLGGPIITTQVTIPKDLAGSIIGKGGQRIKQIRHESGASIKIDEPLEGSEDRIITITGTQDQIQNAQYLLQNSVKQYSGKFF","proteinLength":"Full Length","predictedMolecularWeight":"77.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":463,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P61978","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Heterogeneous nuclear ribonucleoprotein K (hnRNP K) is a multifunctional protein that plays a critical role in RNA metabolism including RNA splicing translation and transcription. Alternate names for hnRNP K include synkaryon-associated protein and hnRNP KurB. It has a molecular mass of approximately 51 kDa and is ubiquitously expressed in the nucleus and cytoplasm of eukaryotic cells. This protein features three KH (K homology) domains that enable it to interact with nucleic acids and other proteins.
Biological function summary

HnRNP K serves as a transcriptional regulator and participates in the assembly of ribonucleoprotein complexes. As part of the ribonucleoprotein complex it interacts with both RNA and DNA influencing various gene expression pathways. It also modulates the activity of several transcription factors including c-Myc and has been implicated in cell proliferation and differentiation. This protein's versatile functions make it an important component in various cellular processes.

Pathways

HnRNP K plays an important role in signal transduction and the p53 pathway. In the p53 pathway hnRNP K acts as a coactivator enhancing the transcriptional activity of the tumor suppressor p53 and influencing cell cycle regulation and apoptosis. Additionally hnRNP K interacts with several proteins including the c-Myc oncogene to regulate gene expression associated with growth and cell cycle progression.

HnRNP K has been linked to cancer and neurological disorders. Its interaction with c-Myc makes it relevant in various cancers where c-Myc is dysregulated contributing to unchecked cell proliferation. Furthermore alterations in hnRNP K expression or function have been associated with neurodegenerative disorders where it might interact with proteins like TDP-43 or FUS affecting neuronal function and survival.
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Specifications

Form

Liquid

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General info

Function

One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA. Plays an important role in p53/TP53 response to DNA damage, acting at the level of both transcription activation and repression. When sumoylated, acts as a transcriptional coactivator of p53/TP53, playing a role in p21/CDKN1A and 14-3-3 sigma/SFN induction (By similarity). As far as transcription repression is concerned, acts by interacting with long intergenic RNA p21 (lincRNA-p21), a non-coding RNA induced by p53/TP53. This interaction is necessary for the induction of apoptosis, but not cell cycle arrest. As part of a ribonucleoprotein complex composed at least of ZNF827, HNRNPL and the circular RNA circZNF827 that nucleates the complex on chromatin, may negatively regulate the transcription of genes involved in neuronal differentiation (PubMed : 33174841).

Post-translational modifications

Arg-296 and Arg-299 are dimethylated, probably to asymmetric dimethylarginine.. Sumoylated by CBX4. Sumoylation is increased upon DNA damage, such as that produced by doxorubicin, etoposide, UV light and camptothecin, due to enhanced CBX4 phosphorylation by HIPK2 under these conditions.. Ubiquitinated by MDM2. Doxorubicin treatment does not affect monoubiquitination, but slightly decreases HNRNPK poly-ubiquitination.. O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.

Subcellular localisation

Nucleus

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Product protocols

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Target data

One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA. Plays an important role in p53/TP53 response to DNA damage, acting at the level of both transcription activation and repression. When sumoylated, acts as a transcriptional coactivator of p53/TP53, playing a role in p21/CDKN1A and 14-3-3 sigma/SFN induction (By similarity). As far as transcription repression is concerned, acts by interacting with long intergenic RNA p21 (lincRNA-p21), a non-coding RNA induced by p53/TP53. This interaction is necessary for the induction of apoptosis, but not cell cycle arrest. As part of a ribonucleoprotein complex composed at least of ZNF827, HNRNPL and the circular RNA circZNF827 that nucleates the complex on chromatin, may negatively regulate the transcription of genes involved in neuronal differentiation (PubMed : 33174841).
See full target information HNRNPK
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Neuroscience bulletin 39:947-961 PubMed36637791

2023

CircFhit Modulates GABAergic Synaptic Transmission via Regulating the Parental Gene Fhit Expression in the Spinal Dorsal Horn in a Rat Model of Neuropathic Pain.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Xu,Zhen-Yu Li,Meng Liu,Su-Bo Zhang,Huan-Huan Ding,Jia-Yan Wu,Su-Yan Lin,Jun Liu,Jia-You Wei,Xue-Qin Zhang,Wen-Jun Xin

Nature communications 13:4865 PubMed36038571

2022

HnRNPK maintains single strand RNA through controlling double-strand RNA in mammalian cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sagar Mahale,Meenakshi Setia,Bharat Prajapati,Santhilal Subhash,Mukesh Pratap Yadav,Subazini Thankaswamy Kosalai,Ananya Deshpande,Jagannath Kuchlyan,Mirco Di Marco,Fredrik Westerlund,L Marcus Wilhelmsson,Chandrasekhar Kanduri,Meena Kanduri

Nature communications 10:2071 PubMed31061501

2019

The transcription factor CBFB suppresses breast cancer through orchestrating translation and transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Navdeep Malik,Hualong Yan,Nellie Moshkovich,Murali Palangat,Howard Yang,Vanesa Sanchez,Zhuo Cai,Tyler J Peat,Shunlin Jiang,Chengyu Liu,Maxwell Lee,Beverly A Mock,Stuart H Yuspa,Daniel Larson,Lalage M Wakefield,Jing Huang
View all publications
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