Recombinant Human hnRNP U/p120 protein (GST tag N-Terminus)

Specifications

Form

Liquid

General info

Function

DNA- and RNA-binding protein involved in several cellular processes such as nuclear chromatin organization, telomere-length regulation, transcription, mRNA alternative splicing and stability, Xist-mediated transcriptional silencing and mitotic cell progression (PubMed : 10490622, PubMed : 18082603, PubMed : 19029303, PubMed : 22325991, PubMed : 25986610, PubMed : 28622508). Plays a role in the regulation of interphase large-scale gene-rich chromatin organization through chromatin-associated RNAs (caRNAs) in a transcription-dependent manner, and thereby maintains genomic stability (PubMed : 1324173, PubMed : 28622508, PubMed : 8174554). Required for the localization of the long non-coding Xist RNA on the inactive chromosome X (Xi) and the subsequent initiation and maintenance of X-linked transcriptional gene silencing during X-inactivation (By similarity). Plays a role as a RNA polymerase II (Pol II) holoenzyme transcription regulator (PubMed : 10490622, PubMed : 15711563, PubMed : 19617346, PubMed : 23811339, PubMed : 8174554, PubMed : 9353307). Promotes transcription initiation by direct association with the core-TFIIH basal transcription factor complex for the assembly of a functional pre-initiation complex with Pol II in a actin-dependent manner (PubMed : 10490622, PubMed : 15711563). Blocks Pol II transcription elongation activity by inhibiting the C-terminal domain (CTD) phosphorylation of Pol II and dissociates from Pol II pre-initiation complex prior to productive transcription elongation (PubMed : 10490622). Positively regulates CBX5-induced transcriptional gene silencing and retention of CBX5 in the nucleus (PubMed : 19617346). Negatively regulates glucocorticoid-mediated transcriptional activation (PubMed : 9353307). Key regulator of transcription initiation and elongation in embryonic stem cells upon leukemia inhibitory factor (LIF) signaling (By similarity). Involved in the long non-coding RNA H19-mediated Pol II transcriptional repression (PubMed : 23811339). Participates in the circadian regulation of the core clock component BMAL1 transcription (By similarity). Plays a role in the regulation of telomere length (PubMed : 18082603). Plays a role as a global pre-mRNA alternative splicing modulator by regulating U2 small nuclear ribonucleoprotein (snRNP) biogenesis (PubMed : 22325991). Plays a role in mRNA stability (PubMed : 17174306, PubMed : 17289661, PubMed : 19029303). Component of the CRD-mediated complex that promotes MYC mRNA stabilization (PubMed : 19029303). Enhances the expression of specific genes, such as tumor necrosis factor TNFA, by regulating mRNA stability, possibly through binding to the 3'-untranslated region (UTR) (PubMed : 17174306). Plays a role in mitotic cell cycle regulation (PubMed : 21242313, PubMed : 25986610). Involved in the formation of stable mitotic spindle microtubules (MTs) attachment to kinetochore, spindle organization and chromosome congression (PubMed : 21242313). Phosphorylation at Ser-59 by PLK1 is required for chromosome alignement and segregation and progression through mitosis (PubMed : 25986610). Also contributes to the targeting of AURKA to mitotic spindle MTs (PubMed : 21242313). Binds to double- and single-stranded DNA and RNA, poly(A), poly(C) and poly(G) oligoribonucleotides (PubMed : 1628625, PubMed : 8068679, PubMed : 8174554, PubMed : 9204873, PubMed : 9405365). Binds to chromatin-associated RNAs (caRNAs) (PubMed : 28622508). Associates with chromatin to scaffold/matrix attachment region (S/MAR) elements in a chromatin-associated RNAs (caRNAs)-dependent manner (PubMed : 10671544, PubMed : 11003645, PubMed : 11909954, PubMed : 1324173, PubMed : 28622508, PubMed : 7509195, PubMed : 9204873, PubMed : 9405365). Binds to the Xist RNA (PubMed : 26244333). Binds the long non-coding H19 RNA (PubMed : 23811339). Binds to SMN1/2 pre-mRNAs at G/U-rich regions (PubMed : 22325991). Binds to small nuclear RNAs (snRNAs) (PubMed : 22325991). Binds to the 3'-UTR of TNFA mRNA (PubMed : 17174306). Binds (via RNA-binding RGG-box region) to the long non-coding Xist RNA; this binding is direct and bridges the Xist RNA and the inactive chromosome X (Xi) (By similarity). Also negatively regulates embryonic stem cell differentiation upon LIF signaling (By similarity). Required for embryonic development (By similarity). Binds to brown fat long non-coding RNA 1 (Blnc1); facilitates the recruitment of Blnc1 by ZBTB7B required to drive brown and beige fat development and thermogenesis (By similarity).. (Microbial infection) Negatively regulates immunodeficiency virus type 1 (HIV-1) replication by preventing the accumulation of viral mRNA transcripts in the cytoplasm.

Post-translational modifications

Cleaved at Asp-100 by CASP3 during T-cell apoptosis, resulting in a loss of DNA- and chromatin-binding activities (PubMed:10671544, PubMed:9405365).. Extensively phosphorylated (PubMed:7993898). Phosphorylated on Ser-59 by PLK1 and dephosphorylated by protein phosphatase 2A (PP2A) in mitosis (PubMed:25986610).. Arg-739 is dimethylated, probably to asymmetric dimethylarginine (Ref.8). Arg-733 is dimethylated, probably to asymmetric dimethylarginine (By similarity).. Citrullinated by PADI4.