Recombinant Human HNRNPM protein (Tagged) is a Human Full Length protein, in the 2 to 730 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
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- Scientific reports 12:105682022SILAC kinase screen identifies potential MASTL substrates.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKamila A Marzec,Samuel Rogers,Rachael McCloy,Benjamin L Parker,David E James,D Neil Watkins,Andrew BurgessPubMed 35732702
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
A A G V E A A A E V A A T E I K M E E E S G A P G V P S G N G A P G P K G E G E R P A Q N E K R K E K N I K R G G N R F E P Y A N P T K R Y R A F I T N I P F D V K W Q S L K D L V K E K V G E V T Y V E L L M D A E G K S R G C A V V E F K M E E S M K K A A E V L N K H S L S G R P L K V K E D P D G E H A R R A M Q K V M A T T G G M G M G P G G P G M I T I P P S I L N N P N I P N E I I H A L Q A G R L G S T V F V A N L D Y K V G W K K L K E V F S M A G V V V R A D I L E D K D G K S R G I G T V T F E Q S I E A V Q A I S M F N G Q L L F D R P M H V K M D E R A L P K G D F F P P E R P Q Q L P H G L G G I G M G L G P G G Q P I D A N H L N K G I G M G N I G P A G M G M E G I G F G I N K M G G M E G P F G G G M E N M G R F G S G M N M G R I N E I L S N A L K R G E I I A K Q G G G G G G G S V P G I E R M G P G I D R L G G A G M E R M G A G L G H G M D R V G S E I E R M G L V M D R M G S V E R M G S G I E R M G P L G L D H M A S S I E R M G Q T M E R I G S G V E R M G A G M G F G L E R M A A P I D R V G Q T I E R M G S G V E R M G P A I E R M G L S M E R M V P A G M G A G L E R M G P V M D R M A T G L E R M G A N N L E R M G L E R M G A N S L E R M G L E R M G A N S L E R M G P A M G P A L G A G I E R M G L A M G G G G G A S F D R A I E M E R G N F G G S F A G S F G G A G G H A P G V A R K A C Q I F V R N L P F D F T W K M L K D K F N E C G H V L Y A D I K M E N G K S K G C G V V K F E S P E V A E R A C R M M N G M K L S G R E I D V R I D R N A
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Pre-mRNA binding protein in vivo, binds avidly to poly(G) and poly(U) RNA homopolymers in vitro. Involved in splicing. Acts as a receptor for carcinoembryonic antigen in Kupffer cells, may initiate a series of signaling events leading to tyrosine phosphorylation of proteins and induction of IL-1 alpha, IL-6, IL-10 and tumor necrosis factor alpha cytokines.
Alternative names
HNRPM, NAGR1, HNRNPM, Heterogeneous nuclear ribonucleoprotein M, hnRNP M
Recommended products
Recombinant Human HNRNPM protein (Tagged) is a Human Full Length protein, in the 2 to 730 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P52272-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- A A G V E A A A E V A A T E I K M E E E S G A P G V P S G N G A P G P K G E G E R P A Q N E K R K E K N I K R G G N R F E P Y A N P T K R Y R A F I T N I P F D V K W Q S L K D L V K E K V G E V T Y V E L L M D A E G K S R G C A V V E F K M E E S M K K A A E V L N K H S L S G R P L K V K E D P D G E H A R R A M Q K V M A T T G G M G M G P G G P G M I T I P P S I L N N P N I P N E I I H A L Q A G R L G S T V F V A N L D Y K V G W K K L K E V F S M A G V V V R A D I L E D K D G K S R G I G T V T F E Q S I E A V Q A I S M F N G Q L L F D R P M H V K M D E R A L P K G D F F P P E R P Q Q L P H G L G G I G M G L G P G G Q P I D A N H L N K G I G M G N I G P A G M G M E G I G F G I N K M G G M E G P F G G G M E N M G R F G S G M N M G R I N E I L S N A L K R G E I I A K Q G G G G G G G S V P G I E R M G P G I D R L G G A G M E R M G A G L G H G M D R V G S E I E R M G L V M D R M G S V E R M G S G I E R M G P L G L D H M A S S I E R M G Q T M E R I G S G V E R M G A G M G F G L E R M A A P I D R V G Q T I E R M G S G V E R M G P A I E R M G L S M E R M V P A G M G A G L E R M G P V M D R M A T G L E R M G A N N L E R M G L E R M G A N S L E R M G L E R M G A N S L E R M G P A M G P A L G A G I E R M G L A M G G G G G A S F D R A I E M E R G N F G G S F A G S F G G A G G H A P G V A R K A C Q I F V R N L P F D F T W K M L K D K F N E C G H V L Y A D I K M E N G K S K G C G V V K F E S P E V A E R A C R M M N G M K L S G R E I D V R I D R N A
- Accession
- P52272
- Protein length
- Full Length
- Predicted molecular weight
- 93.4 kDa
- Amino acids
- 2 to 730
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Pre-mRNA binding protein in vivo, binds avidly to poly(G) and poly(U) RNA homopolymers in vitro. Involved in splicing. Acts as a receptor for carcinoembryonic antigen in Kupffer cells, may initiate a series of signaling events leading to tyrosine phosphorylation of proteins and induction of IL-1 alpha, IL-6, IL-10 and tumor necrosis factor alpha cytokines.
- Post-translational modifications
Sumoylated.
- Subcellular localisation
- Nucleus, Nucleolus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The heterogeneous nuclear ribonucleoprotein M (HNRNPM) is a multifunctional RNA-binding protein also known as glycoprotein p37. It belongs to the hnRNP family. HNRNPM has a molecular mass of approximately 72 kDa. This protein is ubiquitously expressed in various tissues with higher levels in organs like the brain kidney and liver. HNRNPM localizes mainly in the nucleus but shuttles between the nucleus and cytoplasm depending on its phosphorylation state and the cellular context.
- Biological function summary
HnRNP M plays key roles in RNA splicing RNA stability and the transport of mRNA molecules from the nucleus to the cytoplasm. It acts as part of the spliceosome complex and interacts with pre-mRNA to regulate alternative splicing which is essential for generating protein diversity. HNRNPM influences the exon inclusion or exclusion in the pre-mRNA affecting the formation of mature mRNA. This regulation capability connects it with processes such as gene expression and cellular response to external signals.
- Pathways
HNRNPM has significant roles in RNA splicing and processing pathways. Its involvement in alternative splicing links it to the regulation of the MAPK/ERK signaling pathway. In these pathways HNRNPM coordinates with other proteins like SR proteins and other hnRNP family members ensuring efficient mRNA maturation and transport. These activities highlight its importance in maintaining proper cellular functioning and responding to signaling events.
- Associated diseases and disorders
Research connects HNRNPM to cancer and neurodegenerative diseases. Abnormal expression or mutations in HNRNPM associate with tumor progression metastasis and poor prognosis in various cancers. It often interacts with proteins like E-cadherin and beta-catenin impacting cell adhesion and migration. Additionally dysregulation of HNRNPM expression might contribute to the pathogenesis of neurodegenerative conditions like Alzheimer's disease potentially linking it to proteins such as amyloid precursor protein (APP) through altered RNA processing activities.
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SDS-PAGE - Recombinant Human HNRNPM protein (Tagged) (ab226407)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis of ab226407 with 5% enrichment gel and 15% separation gel.
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