Recombinant Human Homeobox protein SIX6 is a Human Full Length protein, in the 1 to 246 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Images
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M F Q L P I L N F S P Q Q V A G V C E T L E E S G D V E R L G R F L W S L P V A P A A C E A L N K N E S V L R A R A I V A F H G G N Y R E L Y H I L E N H K F T K E S H A K L Q A L W L E A H Y Q E A E K L R G R P L G P V D K Y R V R K K F P L P R T I W D G E Q K T H C F K E R T R N L L R E W Y L Q D P Y P N P S K K R E L A Q A T G L T P T Q V G N W F K N R R Q R D R A A A A K N R L Q Q Q V L S Q G S G R A L R A E G D G T P E V L G V A T S P A A S L S S K A A T S A I S I T S S D S E C D I
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Alternative names
OPTX2, SIX9, SIX6, Homeobox protein SIX6, Homeodomain protein OPTX2, Optic homeobox 2, Sine oculis homeobox homolog 6
Recommended products
Recombinant Human Homeobox protein SIX6 is a Human Full Length protein, in the 1 to 246 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- O95475-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 40% Glycerol (glycerin, glycerine), 2.34% Sodium chloride, 0.32% Tris HCl, 0.08% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.06% EDTA
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M F Q L P I L N F S P Q Q V A G V C E T L E E S G D V E R L G R F L W S L P V A P A A C E A L N K N E S V L R A R A I V A F H G G N Y R E L Y H I L E N H K F T K E S H A K L Q A L W L E A H Y Q E A E K L R G R P L G P V D K Y R V R K K F P L P R T I W D G E Q K T H C F K E R T R N L L R E W Y L Q D P Y P N P S K K R E L A Q A T G L T P T Q V G N W F K N R R Q R D R A A A A K N R L Q Q Q V L S Q G S G R A L R A E G D G T P E V L G V A T S P A A S L S S K A A T S A I S I T S S D S E C D I
- Accession
- O95475
- Protein length
- Full Length
- Predicted molecular weight
- 30.1 kDa
- Amino acids
- 1 to 246
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab134617 is purified using conventional chromatography techniques.
General info
- Function
May be involved in eye development.
- Sequence similarities
Belongs to the SIX/Sine oculis homeobox family.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Homeobox protein SIX6 also known as Optx2 or Six9 plays an important role in developmental processes. It is a part of the SIX homeobox family of transcription factors and has a molecular mass of approximately 29 kDa. SIX6 influences the formation and differentiation of specific tissues especially in the eye and brain. This protein is expressed in the retina pituitary gland and forebrain of developing organisms indicating its involvement in early developmental stages and neuronal development.
- Biological function summary
SIX6 influences the regulation of gene expression essential for proper eye and retinal development. It acts as a transcription factor meaning it binds to DNA and modulates transcription of particular target genes. While it is not part of a larger complex SIX6 still interacts with cofactors to exert precise control over genetic programs during development. Its expression ensures that specific cellular pathways are activated or repressed essential for proper differentiation and tissue morphogenesis.
- Pathways
SIX6 is central to the Wnt signaling pathway and the Sonic Hedgehog signaling pathway both of which are important for cell differentiation and proliferation. In these pathways SIX6 works with other proteins such as beta-catenin facilitating the activation of genes important for tissue patterning. SIX6 interplays with transcription factors like Pax6 which together guide the developmental processes of optic and neuroectodermal tissues highlighting its strategic position in early developmental signaling cascades.
- Associated diseases and disorders
SIX6 has been linked to ocular diseases and brain development disorders. Mutations or dysregulation of SIX6 are associated with aniridia a rare eye disorder and optic nerve hypoplasia which leads to vision impairment. Additionally its aberrant expression can connect with other proteins like PAX6 in these disorders where both are important for eye development thereby compounding developmental anomalies. Understanding the function and regulation of SIX6 can help in unraveling the molecular basis of these conditions and in developing targeted therapeutic strategies.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
SDS-PAGE - Recombinant Human Homeobox protein SIX6 (ab134617)
15% SDS-PAGE analysis of 3 µg ab134617.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com