Recombinant Human Homer1 protein is a Human Full Length protein, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Images
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M R G S H H H H H H G M A S M T G G Q Q M G R D L Y D D D D K D R W G S H M G E Q P I F S T R A H V F Q I D P N T K K N W V P T S K H A V T V S Y F Y D S T R N V Y R I I S L D G S K A I I N S T I T P N M T F T K T S Q K F G Q W A D S R A N T V Y G L G F S S E H H L S K F A E K F Q E F K E A A R L A K E K S Q E K M E L T S T P S Q E S A G G D L Q S P L T P E S I N G T D D E R T P D V T Q N S E P R A E P T Q N A L P F S H S S A I S K H W E A E L A T L K G N N A K L T A A L L E S T A N V K Q W K Q Q L A A Y Q E E A E R L H K R V T E L E C V S S Q A N A V H T H K T E L N Q T I Q E L E E T L K L K E E E I E R L K Q E I D N A R E L Q E Q R D S L T Q K L Q E V E I R N K D L E G Q L S D L E Q R L E K S Q N E Q E A F R N N L K T L L E I L D G K I F E L T E L R D N L A K L L E C S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, it aids the coupling of surface receptors to intracellular calcium release. May also couple GRM1 to PI3 kinase through its interaction with AGAP2. Isoform 1 regulates the trafficking and surface expression of GRM5. Isoform 3 acts as a natural dominant negative, in dynamic competition with constitutively expressed isoform 1 to regulate synaptic metabotropic glutamate function. Isoform 3, may be involved in the structural changes that occur at synapses during long-lasting neuronal plasticity and development. Forms a high-order complex with SHANK1, which in turn is necessary for the structural and functional integrity of dendritic spines (By similarity). Negatively regulates T cell activation by inhibiting the calcineurin-NFAT pathway. Acts by competing with calcineurin/PPP3CA for NFAT protein binding, hence preventing NFAT activation by PPP3CA (PubMed:18218901).
Alternative names
SYN47, HOMER1, Homer protein homolog 1, Homer-1
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Recombinant Human Homer1 protein is a Human Full Length protein, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q86YM7-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M R G S H H H H H H G M A S M T G G Q Q M G R D L Y D D D D K D R W G S H M G E Q P I F S T R A H V F Q I D P N T K K N W V P T S K H A V T V S Y F Y D S T R N V Y R I I S L D G S K A I I N S T I T P N M T F T K T S Q K F G Q W A D S R A N T V Y G L G F S S E H H L S K F A E K F Q E F K E A A R L A K E K S Q E K M E L T S T P S Q E S A G G D L Q S P L T P E S I N G T D D E R T P D V T Q N S E P R A E P T Q N A L P F S H S S A I S K H W E A E L A T L K G N N A K L T A A L L E S T A N V K Q W K Q Q L A A Y Q E E A E R L H K R V T E L E C V S S Q A N A V H T H K T E L N Q T I Q E L E E T L K L K E E E I E R L K Q E I D N A R E L Q E Q R D S L T Q K L Q E V E I R N K D L E G Q L S D L E Q R L E K S Q N E Q E A F R N N L K T L L E I L D G K I F E L T E L R D N L A K L L E C S
- Accession
- Q86YM7
- Protein length
- Full Length
- Nature
- Recombinant
Specifications
- Form
- Liquid
- Additional notes
ab85334 is purified by using conventional chromatography techniques.
General info
- Function
Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, it aids the coupling of surface receptors to intracellular calcium release. May also couple GRM1 to PI3 kinase through its interaction with AGAP2. Isoform 1 regulates the trafficking and surface expression of GRM5. Isoform 3 acts as a natural dominant negative, in dynamic competition with constitutively expressed isoform 1 to regulate synaptic metabotropic glutamate function. Isoform 3, may be involved in the structural changes that occur at synapses during long-lasting neuronal plasticity and development. Forms a high-order complex with SHANK1, which in turn is necessary for the structural and functional integrity of dendritic spines (By similarity). Negatively regulates T cell activation by inhibiting the calcineurin-NFAT pathway. Acts by competing with calcineurin/PPP3CA for NFAT protein binding, hence preventing NFAT activation by PPP3CA (PubMed:18218901).
- Sequence similarities
Belongs to the Homer family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Homer1 also known as HOMER is a scaffolding protein with a mass of approximately 45 kDa. It functions in organizing receptor signaling complexes notably interacting with metabotropic glutamate receptors (mGluRs) and certain transient receptor potential (TRP) channels. Homer1 exists mainly in the postsynaptic density of excitatory synapses in the brain indicating its strong expression in neuronal tissues. It has multiple isoforms with the isoform 1a often being highlighted in various research contexts.
- Biological function summary
Homer1 plays an important role in synaptic plasticity and intracellular calcium signaling. As part of a complex Homer1 aids in linking mGluRs to other proteins stabilizing receptor signaling and contributing to the regulation of calcium homeostasis within the cell. This scaffolding action has implications for the modulation of signal transduction pathways that maintain neuronal communication and synaptic efficacy.
- Pathways
Homer1 interacts significantly with the mGluR signaling pathway and the calcium signaling pathway. These interactions place Homer1 at a nexus of neuronal signal propagation. Related proteins in this context include the mGluR1 and mGluR5 which in sync with Homer1 form a network ensuring proper synaptic response to neurotransmitters. TRPC channels are also closely related as Homer1 helps modulate their activity through dynamic assembly with mGluR complexes.
- Associated diseases and disorders
Homer1 shows involvement in various neurological conditions such as schizophrenia and autism spectrum disorders. Abnormalities in Homer1 expression or function are linked to disrupted synaptic signaling which contributes to the pathophysiology of these disorders. Within these disease frameworks proteins like mGluR5 collaborate with Homer1 and serve as focal points in understanding the disrupted signaling mechanisms underlying these conditions.
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1 product image
SDS-PAGE - Recombinant Human Homer1 protein (ab85334)
15% SDS-PAGE showing ab85334 at approximately 44.5kDa (3μg).
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