Recombinant Human HPRT protein is a Human Full Length protein, in the 1 to 218 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS, WB.
Images
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS, WB
- Biologically active
- No
Amino acid sequence
< i > M G S S H H H H H H S S G L V P R G S H < / i > M A T R S P G V V I S D D E P G Y D L D L F C I P N H Y A E D L E R V F I P H G L I M D R T E R L A R D V M K E M G G H H I V A L C V L K G G Y K F F A D L L D Y I K A L N R N S D R S I P M T V D F I R L K S Y C N D Q S T G D I K V I G G D D L S T L T G K N V L I V E D I I D T G K T M Q T L L S L V R Q Y N P K M V K V A S L L V K R T P R S V G Y K P D F V G F E I P D K F V V G Y A L D Y N E Y F R D L N H V C V I S E T G K A K Y K A
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes ab97411 can be used as a WB positive control in conjunction with Anti-HPRT antibody ab10479. |
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Target data
Function
Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway.
Alternative names
HPRT, HPRT1, Hypoxanthine-guanine phosphoribosyltransferase, HGPRT, HGPRTase
Recommended products
Recombinant Human HPRT protein is a Human Full Length protein, in the 1 to 218 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS, WB.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS, WB
- Biologically active
- No
- Accession
- P00492-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.316% Tris HCl
Sequence info
Amino acid sequence
- < i > M G S S H H H H H H S S G L V P R G S H < / i > M A T R S P G V V I S D D E P G Y D L D L F C I P N H Y A E D L E R V F I P H G L I M D R T E R L A R D V M K E M G G H H I V A L C V L K G G Y K F F A D L L D Y I K A L N R N S D R S I P M T V D F I R L K S Y C N D Q S T G D I K V I G G D D L S T L T G K N V L I V E D I I D T G K T M Q T L L S L V R Q Y N P K M V K V A S L L V K R T P R S V G Y K P D F V G F E I P D K F V V G Y A L D Y N E Y F R D L N H V C V I S E T G K A K Y K A
- Accession
- P00492
- Protein length
- Full Length
- Predicted molecular weight
- 26.7 kDa
- Amino acids
- 1 to 218
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab97411 is purified using conventional chromatography techniques.
General info
- Function
Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway.
- Sequence similarities
Belongs to the purine/pyrimidine phosphoribosyltransferase family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
SDS-PAGE - Recombinant Human HPRT protein (ab97411)
15% SDS-PAGE showing ab97411 at approximately 26.7kDa (3μg).
Western blot - Recombinant Human HPRT protein (ab97411)
All lanes: Western blot - Anti-HPRT antibody (Anti-HPRT antibody ab10479) at 1/500 dilution
All lanes: Western blot - Recombinant Human HPRT protein (ab97411) at 0.01 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 25 kDa
Exposure time: 1min
Western blot - Recombinant Human HPRT protein (ab97411)
All lanes: Western blot - Anti-HPRT antibody (Anti-HPRT antibody ab10479) at 1/500 dilution
All lanes: Western blot - Recombinant Human HPRT protein (ab97411) at 0.01 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 25 kDa
Exposure time: 1min
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