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AB97411

Recombinant Human HPRT protein (His tag N-Terminus)

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Recombinant Human HPRT protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 218 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec, WB.

View Alternative Names

HPRT, HPRT1, Hypoxanthine-guanine phosphoribosyltransferase, HGPRT, HGPRTase

3 Images
Western blot - Recombinant Human HPRT protein (His tag N-Terminus) (AB97411)
  • WB

Unknown

Western blot - Recombinant Human HPRT protein (His tag N-Terminus) (AB97411)

All lanes:

Western blot - Anti-HPRT antibody (<a href='/en-us/products/primary-antibodies/hprt-antibody-ab10479'>ab10479</a>) at 1/500 dilution

All lanes:

Western blot - Recombinant Human HPRT protein (His tag N-Terminus) (ab97411) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 25 kDa

true

Exposure time: 1min

Western blot - Recombinant Human HPRT protein (His tag N-Terminus) (AB97411)
  • WB

Unknown

Western blot - Recombinant Human HPRT protein (His tag N-Terminus) (AB97411)

All lanes:

Western blot - Anti-HPRT antibody (<a href='/en-us/products/primary-antibodies/hprt-antibody-ab10479'>ab10479</a>) at 1/500 dilution

All lanes:

Western blot - Recombinant Human HPRT protein (His tag N-Terminus) (ab97411) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 25 kDa

true

Exposure time: 1min

SDS-PAGE - Recombinant Human HPRT protein (His tag N-Terminus) (AB97411)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human HPRT protein (His tag N-Terminus) (AB97411)

15% SDS-PAGE showing ab97411 at approximately 26.7kDa (3μg).

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec, WB

applications

Biologically active

No

Accession

P00492

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.316% Tris HCl

storage-buffer

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Complementary Products

Cell Lines & Lysates

AB150035

HeLa whole cell lysate

Western blot - HeLa whole cell lysate (AB150035)

  • 0
  • 0
Primary Antibodies

AB10479

Anti-HPRT antibody

KO Validated

Western blot - Anti-HPRT antibody (AB10479)

  • 5
  • 4
Sample Prep & Detection Kits

AB170197

HeLa Whole Cell RIPA Extract

ELISA - HeLa Whole Cell RIPA Extract (AB170197)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>ab97411 can be used as a WB positive control in conjunction with <a href='/en-us/products/primary-antibodies/hprt-antibody-ab10479'>ab10479</a>.</p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMATRSPGVVISDDEPGYDLDLFCIPNHYAEDLERVFIPHGLIMDRTERLARDVMKEMGGHHIVALCVLKGGYKFFADLLDYIKALNRNSDRSIPMTVDFIRLKSYCNDQSTGDIKVIGGDDLSTLTGKNVLIVEDIIDTGKTMQTLLSLVRQYNPKMVKVASLLVKRTPRSVGYKPDFVGFEIPDKFVVGYALDYNEYFRDLNHVCVISETGKAKYKA","proteinLength":"Full Length","predictedMolecularWeight":"26.7 kDa","actualMolecularWeight":null,"aminoAcidEnd":218,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P00492","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Specifications

Form

Liquid

Additional notes

ab97411 is purified using conventional chromatography techniques.

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General info

Function

Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway.

Sequence similarities

Belongs to the purine/pyrimidine phosphoribosyltransferase family.

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Product protocols

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Target data

Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway.
See full target information HPRT1
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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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