JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB313364

Recombinant Human HPSE Protein (His tag)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human HPSE Protein (His tag) is a Human Full Length protein, in the 36 to 543 aa range, expressed in HEK 293 cells, with <95%, <0.005 EU/µg endotoxin level, suitable for Mass Spec, SDS-PAGE.

View Alternative Names

HEP, HPA, HPA1, HPR1, HPSE1, HSE1, HPSE, Heparanase, Endo-glucoronidase, Heparanase-1, Hpa1

2 Images
Mass Spectrometry - Recombinant Human HPSE Protein (His tag) (AB313364)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant Human HPSE Protein (His tag) (AB313364)

Mass determination by ESI-TOF. Predicted MW is 59078.34 (+/- 10Da by ESI-TOF). Observed MW is 59068.26.

SDS-PAGE - Recombinant Human HPSE Protein (His tag) (AB313364)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human HPSE Protein (His tag) (AB313364)

SDS-page analysis of ab313364

Key facts

Purity

<95% HPLC

Endotoxin level

<0.005 EU/µg

Expression system

HEK 293 cells

Tags

His tag C-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

Q9Y251

Animal free

Yes

Carrier free

No

Species

Human

Storage buffer

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

style
left-column

Complementary Products

Reagents, Controls & Accessories

AB325997

Prestained Protein Ladder - Extra broad molecular weight (3-250 kDa, 5-color)

SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (3-250 kDa, 5-color) (AB325997)

  • 0
  • 0
Primary Antibodies

AB288438

Anti-Heparanase 1 antibody [EPR25303-58]

20ul selling size|RabMAb|Recombinant|KO Validated|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heparanase 1 antibody [EPR25303-58] (AB288438)

  • 5
  • 1
Assay Kits

AB256401

Human Heparanase ELISA Kit (HPSE)

Recombinant|SimpleStep

Sandwich ELISA - Human Heparanase ELISA Kit (HPSE) (AB256401)

  • 0
  • 1
Cell Lines & Lysates

AB258456

Human HPSE (Heparanase 1) knockout HeLa cell lysate

Western blot - Human HPSE (Heparanase 1) knockout HeLa cell lysate (AB258456)

  • 0
  • 0
Proteins & Peptides

AB276778

Recombinant Mouse VEGFD protein (His tag)

SDS-PAGE - Recombinant Mouse VEGFD protein (His tag) (AB276778)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":"QDVVDLDFFTQEPLHLVSPSFLSVTIDANLATDPRFLILLGSPKLRTLARGLSPAYLRFGGTKTDFLIFDPKKESTFEERSYWQSQVNQDICKYGSIPPDVEEKLRLEWPYQEQLLLREHYQKKFKNSTYSRSSVDVLYTFANCSGLDLIFGLNALLRTADLQWNSSNAQLLLDYCSSKGYNISWELGNEPNSFLKKADIFINGSQLGEDFIQLHKLLRKSTFKNAKLYGPDVGQPRRKTAKMLKSFLKAGGEVIDSVTWHHYYLNGRTATKEDFLNPDVLDIFISSVQKVFQVVESTRPGKKVWLGETSSAYGGGAPLLSDTFAAGFMWLDKLGLSARMGIEVVMRQVFFGAGNYHLVDENFDPLPDYWLSLLFKKLVGTKVLMASVQGSKRRKLRVYLHCTNTDNPRYKEGDLTLYAINLHNVTKYLRLPYPFSNKQVDKYLLRPLGPHGLLSKSVQLNGLTLKMVDDQTLPPLMEKPLRPGSSLGLPAFSYSFFVIRNAKVAACI","proteinLength":"Full Length","predictedMolecularWeight":"59.1 kDa","actualMolecularWeight":"59.1 kDa","aminoAcidEnd":543,"aminoAcidStart":36,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"Q9Y251","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Heparanase 1 also known as HPA1 or HPSE is an endo-β-D-glucuronidase enzyme with a molecular mass of approximately 50 kDa. It functions mechanically by cleaving heparan sulfate (HS) chains which are long sugar chains found on the extracellular matrix and cell surfaces. This cleavage activity remodels the matrix and influences cell behavior. Heparanase 1 is mainly expressed in the placenta lymphoid tissues and various tumor cells where it plays a role in matrix degradation and cell migration.
Biological function summary

When heparan sulfate is cleaved by Heparanase 1 it causes the release of HS-bound growth factors and cytokines making them available to cells. This enzymatic activity facilitates cell proliferation and angiogenesis affecting processes important for tissue repair and cancer progression. Heparanase 1 does not require a complex for its function but operates as a monomeric enzyme. Its activity is investigated using assays like heparanase activity assays or ELISA for measuring the enzyme's activity in different biological contexts including mouse models.

Pathways

The activity of Heparanase 1 intersects significantly with the regulation of angiogenesis and cell signaling pathways such as the VEGF and FGF pathways. These pathways are important for new blood vessel formation making them significant in both normal development and cancer. Heparanase 1 interacts with proteins such as VEGF which is important for blood vessel growth and integrins which are involved in cell-matrix interactions and signaling.

Heparanase 1 is associated with tumor metastasis and inflammation. Its ability to degrade the extracellular matrix enables cancer cell invasion and metastasis. The enzyme also plays a role in inflammatory diseases where it modulates the immune response. Through these disease pathways it relates to other proteins such as matrix metalloproteinases (MMPs) which also participate in matrix remodeling during tumor progression and inflammation.
style
left-column

Specifications

Form

Lyophilized

style
left-column

General info

Function

Endoglycosidase that cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans. Participates in extracellular matrix (ECM) degradation and remodeling. Selectively cleaves the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying either a 3-O-sulfo or a 6-O-sulfo group. Can also cleave the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying a 2-O-sulfo group, but not linkages between a glucuronic acid unit and a 2-O-sulfated iduronic acid moiety. It is essentially inactive at neutral pH but becomes active under acidic conditions such as during tumor invasion and in inflammatory processes. Facilitates cell migration associated with metastasis, wound healing and inflammation. Enhances shedding of syndecans, and increases endothelial invasion and angiogenesis in myelomas. Acts as a procoagulant by increasing the generation of activation factor X in the presence of tissue factor and activation factor VII. Increases cell adhesion to the extracellular matrix (ECM), independent of its enzymatic activity. Induces AKT1/PKB phosphorylation via lipid rafts increasing cell mobility and invasion. Heparin increases this AKT1/PKB activation. Regulates osteogenesis. Enhances angiogenesis through up-regulation of SRC-mediated activation of VEGF. Implicated in hair follicle inner root sheath differentiation and hair homeostasis.

Sequence similarities

Belongs to the glycosyl hydrolase 79 family.

Post-translational modifications

Proteolytically processed. The cleavage of the 65 kDa form leads to the generation of a linker peptide, and 8 kDa and 50 kDa products. The active form, the 8/50 kDa heterodimer, is resistant to degradation. Complete removal of the linker peptide appears to be a prerequisite to the complete activation of the enzyme.. N-glycosylated. Glycosylation of the 50 kDa subunit appears to be essential for its solubility.

Subcellular localisation

Lysosome membrane

style
left-column

Product protocols

style
left-column

Target data

Endoglycosidase that cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans. Participates in extracellular matrix (ECM) degradation and remodeling. Selectively cleaves the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying either a 3-O-sulfo or a 6-O-sulfo group. Can also cleave the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying a 2-O-sulfo group, but not linkages between a glucuronic acid unit and a 2-O-sulfated iduronic acid moiety. It is essentially inactive at neutral pH but becomes active under acidic conditions such as during tumor invasion and in inflammatory processes. Facilitates cell migration associated with metastasis, wound healing and inflammation. Enhances shedding of syndecans, and increases endothelial invasion and angiogenesis in myelomas. Acts as a procoagulant by increasing the generation of activation factor X in the presence of tissue factor and activation factor VII. Increases cell adhesion to the extracellular matrix (ECM), independent of its enzymatic activity. Induces AKT1/PKB phosphorylation via lipid rafts increasing cell mobility and invasion. Heparin increases this AKT1/PKB activation. Regulates osteogenesis. Enhances angiogenesis through up-regulation of SRC-mediated activation of VEGF. Implicated in hair follicle inner root sheath differentiation and hair homeostasis.
See full target information HPSE
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com