Products
Sample Prep & Detection Kits
Conjugation kitsPurification kitsSample preparation kitsChromogen kitsIHC kitsChIP kitsAccessory Reagents & Controls
Accessory reagents & controlsBiochemicals
BiochemicalsProteins and Peptides
Proteins and peptidesOur latest ELISA kit: Human Tau (phospho T217) - Intracellular
Highly sensitive kit offering the most promising biomarkers for Alzheimer’s disease diagnostics. Learn about all product ranges with our product overviews.
Resources
Featured events
Make new connections at our global events.
Support
Our programs
New Lab Program
Get a head start with our exclusive new lab discount. Enjoy 20% off and free shipping for three months.
New Biotech Program
Just starting out? Get 15% off and free shipping to your lab for six months.
Product promise
Peace of mind that all products perform as stated.
Product reviews
Leave reviews, get rewarded and help your community.
Trial program
Try untested species and applications to earn money off your next order.
Recombinant human HRG protein (Active) is a Human Full Length protein, in the 19 to 525 aa range, expressed in CHO, with >90% purity and suitable for SDS-PAGE, FuncS, HPLC.
Alternative names=Histidine-rich glycoprotein, Histidine-proline-rich glycoprotein, HPRG, HRG
- Purity
>90% SDS-PAGE
- Expression system
CHO cells
- Tags
Tag free
- Applications
SDS-PAGE, FuncS, HPLC
- Biologically active
Yes
- Sequence
V S P T D C S A V E P E A E K A L D L I N K R R R D G Y L F Q L L R I A D A H L D R V E N T T V Y Y L V L D V Q E S D C S V L S R K Y W N D C E P P D S R R P S E I V I G Q C K V I A T R H S H E S Q D L R V I D F N C T T S S V S S A L A N T K D S P V L I D F F E D T E R Y R K Q A N K A L E K Y K E E N D D F A S F R V D R I E R V A R V R G G E G T G Y F V D F S V R N C P R H H F P R H P N V F G F C R A D L F Y D V E A L D L E S P K N L V I N C E V F D P Q E H E N I N G V P P H L G H P F H W G G H E R S S T T K P P F K P H G S R D H H H P H K P H E H G P P P P P D E R D H S H G P P L P Q G P P P L L P M S C S S C Q H A T F G T N G A Q R H S H N N N S S D L H P H K H H S H E Q H P H G H H P H A H H P H E H D T H R Q H P H G H H P H G H H P H G H H P H G H H P H G H H P H C H D F Q D Y G P C D P P P H N Q G H C C H G H G P P P G H L R R R G P G K G P R P F H C R Q I G S V Y R L P P L R K G E V L P L P E A N F P S F P L P H H K H P L K P D N Q P F P Q S V S E S C P G K F K S G F P Q V S M F F T H T F P K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Recommended products
Recombinant human HRG protein (Active) is a Human Full Length protein, in the 19 to 525 aa range, expressed in CHO, with >90% purity and suitable for SDS-PAGE, FuncS, HPLC.
Alternative names=Histidine-rich glycoprotein, Histidine-proline-rich glycoprotein, HPRG, HRG
Key facts
- Purity
>90% SDS-PAGE
- Expression system
CHO cells
- Tags
Tag free
- Applications
SDS-PAGE, FuncS, HPLC
- Biologically active
Yes
- Biological activity
Measured by its ability to support adhesion of Con-A activated MOLT-4 cells to the Recombinant Human HPRG-coated surface. The ED50 for this effect is 1.5-2.0 μg/ml.
- Predicted molecular weight
57.7 kDa
- Amino acids
19 to 525
- Sequence
V S P T D C S A V E P E A E K A L D L I N K R R R D G Y L F Q L L R I A D A H L D R V E N T T V Y Y L V L D V Q E S D C S V L S R K Y W N D C E P P D S R R P S E I V I G Q C K V I A T R H S H E S Q D L R V I D F N C T T S S V S S A L A N T K D S P V L I D F F E D T E R Y R K Q A N K A L E K Y K E E N D D F A S F R V D R I E R V A R V R G G E G T G Y F V D F S V R N C P R H H F P R H P N V F G F C R A D L F Y D V E A L D L E S P K N L V I N C E V F D P Q E H E N I N G V P P H L G H P F H W G G H E R S S T T K P P F K P H G S R D H H H P H K P H E H G P P P P P D E R D H S H G P P L P Q G P P P L L P M S C S S C Q H A T F G T N G A Q R H S H N N N S S D L H P H K H H S H E Q H P H G H H P H A H H P H E H D T H R Q H P H G H H P H G H H P H G H H P H G H H P H G H H P H C H D F Q D Y G P C D P P P H N Q G H C C H G H G P P P G H L R R R G P G K G P R P F H C R Q I G S V Y R L P P L R K G E V L P L P E A N F P S F P L P H H K H P L K P D N Q P F P Q S V S E S C P G K F K S G F P Q V S M F F T H T F P K
- Accession
- P04196-1
- Protein length
Full Length
- Animal free
No
- Nature
Recombinant
- Species
Human
- Concentration
Specifications
- Form
Lyophilized
- Additional notes
Purity is greater than 90% by SDS-PAGE gel and HPLC analyses.
General info
- Function
Plasma glycoprotein that binds a number of ligands such as heme, heparin, heparan sulfate, thrombospondin, plasminogen, and divalent metal ions. Binds heparin and heparin/glycosaminoglycans in a zinc-dependent manner. Binds heparan sulfate on the surface of liver, lung, kidney and heart endothelial cells. Binds to N-sulfated polysaccharide chains on the surface of liver endothelial cells. Inhibits rosette formation. Acts as an adapter protein and is implicated in regulating many processes such as immune complex and pathogen clearance, cell chemotaxis, cell adhesion, angiogenesis, coagulation and fibrinolysis. Mediates clearance of necrotic cells through enhancing the phagocytosis of necrotic cells in a heparan sulfate-dependent pathway. This process can be regulated by the presence of certain HRG ligands such as heparin and zinc ions. Binds to IgG subclasses of immunoglobins containing kappa and lambda light chains with different affinities regulating their clearance and inhibiting the formation of insoluble immune complexes. Tethers plasminogen to the cell surface. Binds T-cells and alters the cell morphology. Modulates angiogenesis by blocking the CD6-mediated antiangiongenic effect of thrombospondins, THBS1 and THBS2. Acts as a regulator of the vascular endothelial growth factor (VEGF) signaling pathway; inhibits endothelial cell motility by reducing VEGF-induced complex formation between PXN/paxillin and ILK/integrin-linked protein kinase and by promoting inhibition of VEGF-induced tyrosine phosphorylation of focal adhesion kinases and alpha-actinins in endothelial cells. Also plays a role in the regulation of tumor angiogenesis and tumor immune surveillance. Normalizes tumor vessels and promotes antitumor immunity by polarizing tumor-associated macrophages, leading to decreased tumor growth and metastasis.
- Post-translational modifications
Proteolytic cleavage produces several HRG fragments which are mostly disulfide-linked and, therefore, not released. Cleavage by plasmin is inhibited in the presence of heparin, zinc ions or in an acidic environment. Cleavage reduces binding of HRG to heparan sulfate, but enhances the ability of HRG to bind and tether plasminogen to the cell surface. On platelet activation, releases a 33 kDa antiangiogenic peptide which encompasses the HRR. Also cleaved in the C-terminal by plasmin.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com