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AB128453

Recombinant Human HSCB protein

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Recombinant Human HSCB protein is a Human Full Length protein, in the 30 to 235 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

DNAJC20, HSC20, HSCB, Iron-sulfur cluster co-chaperone protein HscB, DnaJ homolog subfamily C member 20

1 Images
SDS-PAGE - Recombinant Human HSCB protein (AB128453)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human HSCB protein (AB128453)

15% SDS-PAGE analysis of 3 μg ab128453.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

Q8IWL3

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSHMAASQAGSNYPRCWNCGGPWGPGREDRFFCPQCRALQAPDPTRDYFSLMDCNRSFRVDTAKLQHRYQQLQRLVHPDFFSQRSQTEKDFSEKHSTLVNDAYKTLLAPLSRGLYLLKLHGIEIPERTDYEMDRQFLIEIMEINEKLAEAESEAAMKEIESIVKAKQKEFTDNVSSAFEQDDFEEAKEILTKMRYFSNIEEKIKLKKIPL","proteinLength":"Full Length","predictedMolecularWeight":"26.7 kDa","actualMolecularWeight":null,"aminoAcidEnd":235,"aminoAcidStart":30,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q8IWL3","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Specifications

Form

Liquid

Additional notes

ab128453 was purified using conventional chromatography techniques.

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General info

Function

Iron-sulfur cluster co-chaperone protein HscB, mitochondrial. Acts as a co-chaperone in iron-sulfur cluster assembly in mitochondria (PubMed : 20668094). Required for incorporation of iron-sulfur clusters into SDHB, the iron-sulfur protein subunit of succinate dehydrogenase that is involved in complex II of the mitochondrial electron transport chain (PubMed : 26749241). Recruited to SDHB by interaction with SDHAF1 which first binds SDHB and then recruits the iron-sulfur transfer complex formed by HSC20, HSPA9 and ISCU through direct binding to HSC20 (PubMed : 26749241). Plays an essential role in hematopoiesis (By similarity).. Iron-sulfur cluster co-chaperone protein HscB, cytoplasmic. Acts as a co-chaperone in iron-sulfur cluster assembly in the cytoplasm (PubMed : 29309586). Also mediates complex formation between components of the cytosolic iron-sulfur biogenesis pathway and the CIA targeting complex composed of CIAO1, DIPK1B/FAM69B and MMS19 by binding directly to the scaffold protein ISCU and to CIAO1 (PubMed : 29309586). This facilitates iron-sulfur cluster insertion into a number of cytoplasmic and nuclear proteins including POLD1, ELP3, DPYD and PPAT (PubMed : 29309586).

Sequence similarities

Belongs to the HscB family.

Subcellular localisation

Mitochondrion

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Product protocols

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Target data

Iron-sulfur cluster co-chaperone protein HscB, mitochondrial. Acts as a co-chaperone in iron-sulfur cluster assembly in mitochondria (PubMed : 20668094). Required for incorporation of iron-sulfur clusters into SDHB, the iron-sulfur protein subunit of succinate dehydrogenase that is involved in complex II of the mitochondrial electron transport chain (PubMed : 26749241). Recruited to SDHB by interaction with SDHAF1 which first binds SDHB and then recruits the iron-sulfur transfer complex formed by HSC20, HSPA9 and ISCU through direct binding to HSC20 (PubMed : 26749241). Plays an essential role in hematopoiesis (By similarity).. Iron-sulfur cluster co-chaperone protein HscB, cytoplasmic. Acts as a co-chaperone in iron-sulfur cluster assembly in the cytoplasm (PubMed : 29309586). Also mediates complex formation between components of the cytosolic iron-sulfur biogenesis pathway and the CIA targeting complex composed of CIAO1, DIPK1B/FAM69B and MMS19 by binding directly to the scaffold protein ISCU and to CIAO1 (PubMed : 29309586). This facilitates iron-sulfur cluster insertion into a number of cytoplasmic and nuclear proteins including POLD1, ELP3, DPYD and PPAT (PubMed : 29309586).
See full target information HSCB
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