Recombinant Human HSD17B8 protein is a Human Full Length protein, in the 1 to 261 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M A S Q L Q N R L R S A L A L V T G A G S G I G R A V S V R L A G E G A T V A A C D L D R A A A Q E T V R L L G G P G S K E G P P R G N H A A F Q A D V S E A R A A R C L L E Q V Q A C F S R P P S V V V S C A G I T Q D E F L L H M S E D D W D K V I A V N L K G T F L V T Q A A A Q A L V S N G C R G S I I N I S S I V G K V G N V G Q T N Y A A S K A G V I G L T Q T A A R E L G R H G I R C N S V L P G F I A T P M T Q K V P Q K V V D K I T E M I P M G H L G D P E D V A D V V A F L A S E D S G Y I T G T S V E V T G G L F M
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Required for the solubility and assembly of the heterotetramer 3-ketoacyl-[acyl carrier protein] (ACP) reductase functional complex (KAR or KAR1) that forms part of the mitochondrial fatty acid synthase (mtFAS). Alpha-subunit of the KAR complex that acts as a scaffold protein required for the stability of carbonyl reductase type-4 (CBR4, beta-subunit of the KAR complex) and for its 3-ketoacyl-ACP reductase activity, thereby participating in mitochondrial fatty acid biosynthesis. Catalyzes the NAD-dependent conversion of (3R)-3-hydroxyacyl-CoA into 3-ketoacyl-CoA (3-oxoacyl-CoA) with no chain length preference; this enzymatic activity is not needed for the KAR function (PubMed:19571038, PubMed:25203508, PubMed:30508570). Prefers (3R)-3-hydroxyacyl-CoA over (3S)-3-hydroxyacyl-CoA and displays enzymatic activity only in the presence of NAD(+) (PubMed:19571038). Cooperates with enoyl-CoA hydratase 1 in mitochondria, together they constitute an alternative route to the auxiliary enzyme pathways for the breakdown of Z-PUFA (cis polyunsaturated fatty acid) enoyl-esters (Probable) (PubMed:30508570). NAD-dependent 17-beta-hydroxysteroid dehydrogenase with highest activity towards estradiol (17beta-estradiol or E2). Has very low activity towards testosterone and dihydrotestosterone (17beta-hydroxy-5alpha-androstan-3-one). Primarily an oxidative enzyme, it can switch to a reductive mode determined in the appropriate physiologic milieu and catalyze the reduction of estrone (E1) to form biologically active 17beta-estradiol (PubMed:17978863).
Alternative names
FABGL, HKE6, RING2, SDR30C1, HSD17B8, (3R)-3-hydroxyacyl-CoA dehydrogenase, 17-beta-hydroxysteroid dehydrogenase 8, 3-ketoacyl-[acyl-carrier-protein] reductase alpha subunit, 3-oxoacyl-[acyl-carrier-protein] reductase, Estradiol 17-beta-dehydrogenase 8, Protein Ke6, Short chain dehydrogenase/reductase family 30C member 1, Testosterone 17-beta-dehydrogenase 8, 17-beta-HSD 8, KAR alpha subunit, Ke6
Recommended products
Recombinant Human HSD17B8 protein is a Human Full Length protein, in the 1 to 261 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q92506-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 40% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.316% Tris HCl
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M A S Q L Q N R L R S A L A L V T G A G S G I G R A V S V R L A G E G A T V A A C D L D R A A A Q E T V R L L G G P G S K E G P P R G N H A A F Q A D V S E A R A A R C L L E Q V Q A C F S R P P S V V V S C A G I T Q D E F L L H M S E D D W D K V I A V N L K G T F L V T Q A A A Q A L V S N G C R G S I I N I S S I V G K V G N V G Q T N Y A A S K A G V I G L T Q T A A R E L G R H G I R C N S V L P G F I A T P M T Q K V P Q K V V D K I T E M I P M G H L G D P E D V A D V V A F L A S E D S G Y I T G T S V E V T G G L F M
- Accession
- Q92506
- Protein length
- Full Length
- Predicted molecular weight
- 29.1 kDa
- Amino acids
- 1 to 261
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Purified using conventional chromatography.
General info
- Function
Required for the solubility and assembly of the heterotetramer 3-ketoacyl-[acyl carrier protein] (ACP) reductase functional complex (KAR or KAR1) that forms part of the mitochondrial fatty acid synthase (mtFAS). Alpha-subunit of the KAR complex that acts as a scaffold protein required for the stability of carbonyl reductase type-4 (CBR4, beta-subunit of the KAR complex) and for its 3-ketoacyl-ACP reductase activity, thereby participating in mitochondrial fatty acid biosynthesis. Catalyzes the NAD-dependent conversion of (3R)-3-hydroxyacyl-CoA into 3-ketoacyl-CoA (3-oxoacyl-CoA) with no chain length preference; this enzymatic activity is not needed for the KAR function (PubMed:19571038, PubMed:25203508, PubMed:30508570). Prefers (3R)-3-hydroxyacyl-CoA over (3S)-3-hydroxyacyl-CoA and displays enzymatic activity only in the presence of NAD(+) (PubMed:19571038). Cooperates with enoyl-CoA hydratase 1 in mitochondria, together they constitute an alternative route to the auxiliary enzyme pathways for the breakdown of Z-PUFA (cis polyunsaturated fatty acid) enoyl-esters (Probable) (PubMed:30508570). NAD-dependent 17-beta-hydroxysteroid dehydrogenase with highest activity towards estradiol (17beta-estradiol or E2). Has very low activity towards testosterone and dihydrotestosterone (17beta-hydroxy-5alpha-androstan-3-one). Primarily an oxidative enzyme, it can switch to a reductive mode determined in the appropriate physiologic milieu and catalyze the reduction of estrone (E1) to form biologically active 17beta-estradiol (PubMed:17978863).
- Sequence similarities
Belongs to the short-chain dehydrogenases/reductases (SDR) family.
- Subcellular localisation
- Mitochondrion matrix
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
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1 product image
SDS-PAGE - Recombinant Human HSD17B8 protein (ab105611)
15% SDS PAGE analysis of ab105611 (3 µg).
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